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One-pot green synthesis and characterization of copper oxide nanoparticles with antibacterial and antioxidant properties using Coleus amboinicus

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Abstract

The current research aims to employ an eco-friendly, less toxic, inexpensive method for the synthesis of copper oxide (CuO) nanoparticles with antibacterial and antioxidant potential using leaf extract of Coleus amboinicus which behaves as a reducing and cap** agent. The size, functional group, shape, stability and purity of the as-synthesized CuO nanoparticles were analysed by FT-IR, XRD, TEM, EDX, TGA and zeta potential techniques. All characterization results displayed that the CuO nanoparticles are crystalline, spherical with an average size of 40–60 nm and stable. TGA curve denotes the weight loss of 16.6% that occurred in CuO nanoparticles due to the evaporation of moisture and volatile compounds. Moreover, the antioxidant and antibacterial properties of Coleus amboinicus-mediated CuO nanoparticles were assessed by DPPH assay and agar well diffusion method. Both assays confirmed and revealed that the synthesized CuO nanoparticles have good antibacterial activity against Escherichia coli and Salmonella sp. and antioxidant properties. It can be effectively used as a good antibacterial and antioxidant agent in various medical applications.

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References

  1. Khan I, Saeed K, Khan I (2019) Review nanoparticles: properties, applications and toxicities. Arab J Chem 12(2):908–931

    Article  Google Scholar 

  2. Vanaja M, Annadurai G (2013) Coleus aromaticus leaf extract mediated synthesis of silver nanoparticles and its bactericidal activity. Appl Nanosci 3:217–223

    Article  Google Scholar 

  3. Patel HV, Mangrola AV, Bariya HS, Patel JD (2020) Antibacterial and larvicidal activity of biologically synthesized silver nanoparticles from Bambusa arundinacea leaves extract. Asian J Biol Life sci 9(1):43

    Google Scholar 

  4. Parthasarathy S, Jayacumar S, Chakraborty S, Soundararajan P, Joshi D, Gangwar K, Bhattacharjee A, Venkatesh MPD (2020) Fabrication and characterization of copper nanoparticles by green synthesis approach using Plectranthus amboinicus leaves extract. Research Square

    Book  Google Scholar 

  5. Saranyaadevi K, Subha V, Ravindran RE, Renganathan S (2014) Synthesis and characterization of copper nanoparticle using Capparis zeylanica leaf extract. Int J Chem Tech Res 6(10):4533–4541

    Google Scholar 

  6. Sivaraj R, Rahman PK, Rajiv P, Narendhran S, Venckatesh R (2014) Biosynthesis and characterization of Acalypha indica mediated copper oxide nanoparticles and evaluation of its antimicrobial and anticancer activity. Spectrochim Acta A Mol Biomol Spectrosc 129:255–258

    Article  Google Scholar 

  7. Davarpanah SJ, Karimian R, Goodarzi V, Piri F (2015) Synthesis of copper (II) oxide (CuO) nanoparticles and its application as gas sensor. J Appl Biotechnol Rep 2(4):329–332

    Google Scholar 

  8. Elazab Dr, H.A., 2018. The catalytic activity of copper oxide nanoparticles towards carbon monoxide oxidation catalysis: microwave–assisted synthesis approach.

    Google Scholar 

  9. Trukawka M, Wenelska K, Singer L, Klingeler R, Chen X, Mijowska E (2021) Hollow carbon spheres loaded with uniform dispersion of copper oxide nanoparticles for anode in lithium-ion batteries. J Alloys Compd 853:156700

    Article  Google Scholar 

  10. Yang Z, Yi C, Lv S, Sheng Y, Wen W, Zhang X, Wang S (2019) Development of a lateral flow strip biosensor based on copper oxide nanoparticles for rapid and sensitive detection of HPV16 DNA. Sensors Actuators B Chem 285:326–332

    Article  Google Scholar 

  11. Kayani ZN, Umer M, Riaz S, Naseem S (2015) Characterization of copper oxide nanoparticles fabricated by the sol–gel method. J Electron Mater 44:3704–3709

    Article  Google Scholar 

  12. Luna IZ, Bangladesh Atomic Energy Commission (2015) Preparation and characterization of copper oxide nanoparticles synthesized via chemical precipitation method. Open Access Lib J 2(03):1

    Google Scholar 

  13. Shahsavani, E., Feizi, N. and DEHNO, K.A., 2016. Copper oxide nanoparticles prepared by solid state thermal decomposition: synthesis and characterization.

    Google Scholar 

  14. Sathiyavimal S, Vasantharaj S, Bharathi D, Saravanan M, Manikandan E, Kumar SS, Pugazhendhi A (2018) Biogenesis of copper oxide nanoparticles (CuONPs) using Sida acuta and their incorporation over cotton fabrics to prevent the pathogenicity of Gram negative and Gram positive bacteria. J Photochem Photobiol B Biol 188:126–134

    Article  Google Scholar 

  15. Matsukevich I, Kulak A, Palkhouskaya V, Romanovski V, Jo JH, Aniskevich Y, Mohamed SG (2022) Comparison of different methods for Li2 M Ti3O8 (M–Co, Cu, Zn) synthesis. J Chem Technol Biotechnol 97(4):1021–1026

    Article  Google Scholar 

  16. Romanovski V, Kolosov AY, Khort A, Myasnichenko VS, Podbolotov KB, Savina KG, Sokolov DN, Romanovskaia E, Sdobnyakov NY (2020) Features of Cu-Ni nanoparticle synthesis: experiment and computer simulation. In: Physical and chemical aspects of the study of clusters nanostructures and nanomaterials, vol 12. Tver State University, pp 293–309

    Google Scholar 

  17. Matsukevich IV, Kulak AI, Popkov VI, Romanovski VI, Fayed MG, Mohamed SG (2022) Lithium cobalt titanate with the spinel structure as an anode material for lithium ion batteries. Inorg Mater 58(2):160–164

    Article  Google Scholar 

  18. Cao A, Zhang M, Su X, Romanovski V, Chu S (2022) In situ fabrication of NiS2-decorated graphitic carbon nitride/metal–organic framework nanostructures for photocatalytic H2 evolution. ACS Appl Nano Mater 5(4):5416–5424

    Article  Google Scholar 

  19. Wang H, Wang Y, Liu Z, Luo S, Romanovski V, Huang X, Czech B, Sun H, Li T (2022) Rational construction of micron-sized zero-valent iron/graphene composite for enhanced Cr (VI) removal from aqueous solution. J Environ Chem Eng 10(6):109004

    Article  Google Scholar 

  20. Vanathi P, Rajiv P, Narendhran S, Rajeshwari S, Rahman PK, Venckatesh R (2014) Biosynthesis and characterization of phyto mediated zinc oxide nanoparticles: a green chemistry approach. Mater Lett 134:13–15

    Article  Google Scholar 

  21. Balasubramanian S, Kala SMJ, Pushparaj TL (2020) Biogenic synthesis of gold nanoparticles using Jasminum auriculatum leaf extract and their catalytic, antimicrobial and anticancer activities. J Drug Deliv Sci Technol 57:101620

    Article  Google Scholar 

  22. Castillo-Henríquez L, Alfaro-Aguilar K, Ugalde-Álvarez J, Vega-Fernández L, Montes de Oca-Vásquez G, Vega-Baudrit JR (2020) Green synthesis of gold and silver nanoparticles from plant extracts and their possible applications as antimicrobial agents in the agricultural area. Nanomaterials 10(9):1763

    Article  Google Scholar 

  23. Rajiv P, Vanathi P, Thangamani A (2018) An investigation of phytotoxicity using Eichhornia mediated zinc oxide nanoparticles on Helianthus annuus. Biocatal Agric Biotechnol 16:419–424

    Article  Google Scholar 

  24. Khan MA, Wallace WT, Sambi J, Rogers DT, Littleton JM, Rankin SE, Knutson BL (2020) Nanoharvesting of bioactive materials from living plant cultures using engineered silica nanoparticles. Mater Sci Eng C 106:110190

    Article  Google Scholar 

  25. Srinivasan S, Rani P (2014) Biological synthesis of copper nanoparticles using Pseudomonas fluorescens. Int J Curr Microbiol App Sci 3:374–383

    Google Scholar 

  26. Narayanan KB, Sakthivel N (2010) Phytosynthesis of gold nanoparticles using leaf extract of Coleus amboinicus Lour. Mater Charact 61(11):1232–1238

    Article  Google Scholar 

  27. Jimmy JL (2021) Coleus aromaticus Benth.: an update on its bioactive constituents and medicinal properties. All Life 14(1):756–773

    Article  Google Scholar 

  28. Zhang G, Keita B, Dolbecq A, Mialane P, Sécheresse F, Miserque F, Nadjo L (2007) Green chemistry-type one-step synthesis of silver nanostructures based on MoV–MoVI mixed-valence polyoxometalates. Chem Mater 19(24):5821–5823

    Article  Google Scholar 

  29. Wu S, Rajeshkumar S, Madasamy M, Mahendran V (2020) Green synthesis of copper nanoparticles using Cissus vitiginea and its antioxidant and antibacterial activity against urinary tract infection pathogens. Artif Cells Nanomed Biotechnol 48(1):1153–1158

    Article  Google Scholar 

  30. Vanathi P, Rajiv P, Sivaraj R (2016) Synthesis and characterization of Eichhornia-mediated copper oxide nanoparticles and assessing their antifungal activity against plant pathogens. Bull Mater Sci 39:1165–1170

    Article  Google Scholar 

  31. Akintelu SA, Folorunso AS, Folorunso FA, Oyebamiji AK (2020) Green synthesis of copper oxide nanoparticles for biomedical application and environmental remediation. Heliyon 6(7):e04508

    Article  Google Scholar 

  32. Periakaruppan R, Abed SA, Vanathi P, Kumar JS (2023) Production of biogenic silica nanoparticles by green chemistry approach and assessment of their physicochemical properties and effects on the germination of Sorghum bicolor. Silicon 15:4309–4316

    Article  Google Scholar 

  33. Dong Y, Wang K, Tan Y et al (2018) Synthesis and characterization of pure copper nanostructures using wood inherent architecture as a natural template. Nanoscale Res Lett 13:119

    Article  Google Scholar 

  34. Rajiv P, Bavadharani B, Kumar MN, Vanathi P (2017) Synthesis and characterization of biogenic iron oxide nanoparticles using green chemistry approach and evaluating their biological activities. Biocatal Agric Biotechnol 12:45–49

    Article  Google Scholar 

  35. Periakaruppan R, Chen X, Thangaraj K, Jeyaraj A, Nguyen HH, Yu Y, Hu S, Lu L, Li X (2021) Utilization of tea resources with the production of superparamagnetic biogenic iron oxide nanoparticles and an assessment of their antioxidant activities. J Clean Prod 278:123962

    Article  Google Scholar 

  36. Narendhran S, Rajiv P, Sivaraj R (2016) Toxicity of ZnO nanoparticles on germinating Sesamum indicum (Co-1) and their antibacterial activity. Bull Mater Sci 39:415–421

    Article  Google Scholar 

  37. Gunalan S, Sivaraj R, Venckatesh R (2012) Aloe barbadensis Miller mediated green synthesis of mono-disperse copper oxide nanoparticles: optical properties. Spectrochim Acta A Mol Biomol Spectrosc 97:1140–1144

    Article  Google Scholar 

  38. Ghidan AY, Al-Antary TM, Awwad AM (2016) Green synthesis of copper oxide nanoparticles using Punica granatum peels extract: effect on green peach Aphid. Environ Nanotechnol Monit Manag 6:95–98

    Google Scholar 

  39. Jagathesan G, Rajiv P (2018) Biosynthesis and characterization of iron oxide nanoparticles using Eichhornia crassipes leaf extract and assessing their antibacterial activity. Biocatal Agric Biotechnol 13:90–94

    Article  Google Scholar 

  40. Jadhav S, Gaikwad S, Nimse M, Rajbhoj A (2011) Copper oxide nanoparticles: synthesis, characterization and their antibacterial activity. J Clust Sci 22:121–129

    Article  Google Scholar 

  41. Kumar PV, Shameem U, Kollu P, Kalyani RL, Pammi SVN (2015) Green synthesis of copper oxide nanoparticles using Aloe vera leaf extract and its antibacterial activity against fish bacterial pathogens. BioNanoScience 5:135–139

    Article  Google Scholar 

  42. Prakash S, Elavarasan N, Venkatesan A, Subashini K, Sowndharya M, Sujatha V (2018) Green synthesis of copper oxide nanoparticles and its effective applications in Biginelli reaction, BTB photodegradation and antibacterial activity. Adv Powder Technol 29(12):3315–3326

    Article  Google Scholar 

  43. Polini A, Yang F (2017) Physicochemical characterization of nanofiber composites. In: Nanofiber composites for biomedical applications. Woodhead Publishing, pp 97–115

    Chapter  Google Scholar 

  44. Nasrollahzadeh M, Sajadi SM, Rostami-Vartooni A, Hussin SM (2016) Green synthesis of CuO nanoparticles using aqueous extract of Thymus vulgaris L. leaves and their catalytic performance for N-arylation of indoles and amines. J Colloid Interface Sci 466:113–119

    Article  Google Scholar 

  45. Varughese, A., Kaur, R. and Singh, P., 2020 Green synthesis and characterization of copper oxide nanoparticles using Psidium guajava leaf extract. In IOP Conference Series: Materials Science and Engineering (961, 1, 012011 IOP Publishing.

    Google Scholar 

  46. Honary S, Zahir F (2013) Effect of zeta potential on the properties of nano-drug delivery systems-a review (Part 1). Trop J Pharm Res 12(2):255–264

    Google Scholar 

  47. Baldi F, Marchetto D, Paganelli S, Piccolo O (2011) Bio-generated metal binding polysaccharides as catalysts for synthetic applications and organic pollutant transformations. New Biotechnol 29(1):74–78

    Article  Google Scholar 

  48. Amin F, Khattak B, Alotaibi A, Qasim M, Ahmad I, Ullah R, Bourhia M, Gul A, Zahoor S, Ahmad R (2021) Green synthesis of copper oxide nanoparticles using Aerva javanica leaf extract and their characterization and investigation of in vitro antimicrobial potential and cytotoxic activities. Evid-based Complement Altern. Med 2021:5589703

    Article  Google Scholar 

  49. Ramzan M, Obodo RM, Mukhtar S, Ilyas SZ, Aziz F, Thovhogi N (2021) Green synthesis of copper oxide nanoparticles using Cedrus deodara aqueous extract for antibacterial activity. Materials Today: Proceed 36:576–581

    Google Scholar 

  50. Dobrucka R (2018) Antioxidant and catalytic activity of biosynthesized CuO nanoparticles using extract of Galeopsidis herba. J Inorg Organomet Polym Mater 28:812–819

    Article  Google Scholar 

  51. Peddi P, Ptsrk PR, Rani NU, Tulasi SL (2021) Green synthesis, characterization, antioxidant, antibacterial, and photocatalytic activity of Suaeda maritima (L.) Dumort aqueous extract-mediated copper oxide nanoparticles. J Genet Eng Biotechnol 19(1):1–11

    Article  Google Scholar 

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Authors and Affiliations

  1. Department of Biotechnology, Karpagam Academy of Higher Education, Coimbatore, Tamil Nadu, 641021, India

    Dinesh Kumar Sivaraj & Sreenidhi Sathish Kumar

  2. Department of Biochemistry and Biotechnology, Avinashilingam Institute for Home Science and Higher Education for Women, Coimbatore, Tamil Nadu, 641108, India

    Jeba Sweetly Dharmadhas

  3. Department of Medical Lab Sciences, Prince Sattam Bin Abdulaziz University, Alkharj, 16278, Saudi Arabia

    Noura Al-Dayan

  4. Department of Bioinformatics, Saveetha School of Engineering-Saveetha Institute of Medical and Technical Sciences, Chennai, Tamil Nadu, 602105, India

    Sugapriya Dhanasekaran

  5. Armed Forces Medical Services, Riyadh, 12426, Saudi Arabia

    Saad Hamad Abdullah Aldhayan

  6. Department of Biotechnology, PSG College of Arts & Science, Coimbatore, Tamil Nadu, 641014, India

    Rajiv Periakaruppan

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  1. Dinesh Kumar Sivaraj
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Contributions

Mr. Dinesh Kumar Sivaraj: investigation, data curation and writing, original draft preparation

Ms. Sreenidhi Sathish Kumar: investigation, data curation and writing, original draft preparation

Dr. Jeba Sweetly Dharmadhas: data curation and writing

Dr. Noura Al-Dayan: data curation and writing

Dr. Sugapriya Dhanasekaran: data curation and writing

Dr. Saad Hamad Abdullah Aldhayan: data curation and writing

Dr. Rajiv Periakaruppan: supervision, funding acquisition and project administration

Corresponding author

Correspondence to Rajiv Periakaruppan.

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Sivaraj, D.K., Kumar, S.S., Dharmadhas, J.S. et al. One-pot green synthesis and characterization of copper oxide nanoparticles with antibacterial and antioxidant properties using Coleus amboinicus. Biomass Conv. Bioref. (2023). https://doi.org/10.1007/s13399-023-04799-1

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