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MiRNA-19a and miRNA-19b regulate proliferation of antler cells by targeting TGFBR2

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Abstract

Transforming growth factor (TGF-β) plays a pivotal role in angiogenesis. The purpose of this study was to explore the microRNA-mediated regulation of TGF-β receptor-II (TGFBR2) expression during rapid antler growth and proliferation of antler cells in sika deer. Deep sequencing–based expression analysis of miRNAs on the antler tip tissue was performed. Then, two bioinformatics software were used to analyze TGFBR2 3′-UTR sequence for predicting the matched and differentially expressed miRNAs in different tissues of the antler. The results indicated that miRNA-19a and miRNA-19b exhibited the highest upregulation among differentially expressed miRNAs. We also found that the TGFBR2 3′-UTR contains a binding site for miRNA-19a and miRNA-19b by transfection of wild-type and mutant dual-luciferase reporter vectors into antler cartilage cells. Meanwhile, overexpression of miRNA-19a and miRNA-19b significantly inhibited the proliferation of cartilage cells in vitro, and decreased the expression level of TGFBR2 protein. Furthermore, the expression levels of insulin-like growth factor 1 (IGF-1) and TGF-β2, which were associated with TGFBR2, reduced after transfection of cartilage cells with miRNA-19a and miRNA-19b. Our results indicate the significant roles of miRNA-19a and miRNA-19b in proliferation of antler cells and its potential application.

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Funding

This study was supported by a grant from the National Natural Science Foundation of China (grant nos. 31572372 and 30972083).

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Authors and Affiliations

  1. College of Life Science, Jilin Agriculture University, Changchun, 130118, Jilin Province, China

    Yuduo Yan, Danyang Chen, **angyu Han, Mingxiao Liu & Wei Hu

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  1. Yuduo Yan
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  2. Danyang Chen
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  3. **angyu Han
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  4. Mingxiao Liu
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  5. Wei Hu
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Correspondence to Wei Hu.

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Communicated by: Shuiqiao Yuan

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Yan, Y., Chen, D., Han, X. et al. MiRNA-19a and miRNA-19b regulate proliferation of antler cells by targeting TGFBR2. Mamm Res 65, 339–348 (2020). https://doi.org/10.1007/s13364-019-00469-8

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  • DOI: https://doi.org/10.1007/s13364-019-00469-8

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