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Voltage-gated ion channels in central neurons of Helicoverpa armigera as potential targets for cycloxaprid: a cis-configuration neonicotinoid insecticide

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Abstract

Cycloxaprid is a novel cis-neonicotinoid, mainly acting on the nicotinic acetylcholine receptor; however, it is not clear whether cycloxaprid can act on voltage-gated ion channels. In this study, the effects of cycloxaprid on the sodium, calcium and potassium channels in central neurons acutely dissociated from Helicoverpa armigera Hübner (Lepidoptera: Noctuidae) were investigated by the whole-cell patch clamp technique. With the application of cycloxaprid, the half voltage (V0.5) of activation and inactivation of sodium channels exhibited an obvious hyperpolarizing shift around 4–16 mV and 4–14 mV, respectively. The window currents of sodium channels increased by 35.04–88.89%. The time course of recovery from inactivation was also significantly prolonged by 0.25–0.43 ms. The V0.5 of activation and inactivation of calcium channels exhibited a marked hyperpolarizing shift around 6–9 mV and 13–19 mV, respectively. The window currents of calcium channels increased by 13.82–28.97%. The time course of recovery from inactivation for calcium channels was prolonged by 0.76–16.85 ms, although not significantly. Comparatively, the peak currents and the V0.5 of activation of potassium channels showed no significant change. These results indicate that sodium and calcium channels of H. armigera are potential target sites of cycloxaprid.

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The data supporting the findings of this study are available within the article and its supplementary materials.

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Acknowledgements

This work was supported by grants from National Natural Science Foundation of China (Grant number 31871992 and 31371974).

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  1. **ao Liu and Man Chen contributed equally to this work.

Authors and Affiliations

  1. Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, Nankai University, Tian**, 300071, China

    **ao Liu, Man Chen, Zheng Ma, Yongchuan Yang, Yangxingzi Li & Bingjun He

  2. Agricultural Research and Development Program, Central State University, 1400 Brush Row Road, Wilberforce, OH, USA

    Hongmei Li-Byarlay

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  1. **ao Liu
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Liu, X., Chen, M., Ma, Z. et al. Voltage-gated ion channels in central neurons of Helicoverpa armigera as potential targets for cycloxaprid: a cis-configuration neonicotinoid insecticide. Appl Entomol Zool 58, 149–160 (2023). https://doi.org/10.1007/s13355-023-00816-x

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