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Decreased long noncoding RNA MIR31HG is correlated with poor prognosis and contributes to cell proliferation in gastric cancer

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Tumor Biology

Abstract

Long noncoding RNAs (lncRNAs) are emerging as key regulators governing fundamental biological processes, and their disorder expression involves in the development of several human cancers. MIR31HG, an lncRNA located in 9p21.3 and 2166 bp in length, has been found to be upregulated in breast cancer and contributes to cell proliferation and invasion. However, the expression pattern and biological function of MIR31HG in gastric cancer are still not well documented. In this study, we found that MIR31HG expression is decreased in gastric cancer tissues and associated with larger tumor size and advanced pathological stage. Patients with lower MIR31HG expression had a relatively poor prognosis. Furthermore, ectopic over-expression of MIR31HG could inhibit gastric cancer (GC) cell proliferation both in vitro and in vivo, while knockdown of MIR31HG by small interfering RNA (siRNA) promoted cell proliferation in GC cells partly via regulating E2F1 and p21 expression. Our findings present that decreased MIR31HG is involved in GC development and could be identified as a poor prognostic biomarker in GC patients.

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Acknowledgments

This work was supported by the National Scientific Foundation of China (No. 81301824) and National Scientific Foundation of Jiangsu Provincial of China (BK20141437).

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Authors and Affiliations

  1. Department of Oncology, Second Affiliated Hospital, Nan**g Medical University, Nan**g, China

    Feng-qi Nie

  2. Department of Gastroenterology, Huai’an First People’s Hospital, Nan**g Medical University, Huai’an, China

    Shijie Ma

  3. Department of Biochemistry and Molecular Biology, Nan**g Medical University, 210029, Nan**g, China

    Min **e, Yan-wen Liu, Wei De & **ang-hua Liu

Authors
  1. Feng-qi Nie
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  2. Shijie Ma
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  3. Min **e
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  4. Yan-wen Liu
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  6. **ang-hua Liu
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Corresponding author

Correspondence to **ang-hua Liu.

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This study was approved by the Research Ethics Committee of Nan**g Medical University, China. Informed consents were obtained from all patients.

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Additional information

Feng-qi Nie, Shijie Ma, and Min **e contributed equally to this work and should be regarded as joint first authors.

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Primers and siRNA sequence. (XLS 24 kb)

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Nie, Fq., Ma, S., **e, M. et al. Decreased long noncoding RNA MIR31HG is correlated with poor prognosis and contributes to cell proliferation in gastric cancer. Tumor Biol. 37, 7693–7701 (2016). https://doi.org/10.1007/s13277-015-4644-z

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  • DOI: https://doi.org/10.1007/s13277-015-4644-z

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