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miR-211 promotes non-small cell lung cancer proliferation by targeting SRCIN1

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Tumor Biology

Abstract

MicroRNAs (miRNAs) are a class of small non-coding RNAs that, when dysregulated, are involved in the initiation and progression of various cancers, including lung cancer, in humans. In the current study, qRT-PCR was performed to measure miR-211 expression in human non-small cell lung cancer (NSCLC) cell lines and tissues. Cell proliferation, cell cycle, colony formation, and invasion were performed to detect the functional role of miR-211 in human NSCLC cell line. We used luciferase reporter assay to find the potential target of miR-211. We found that miR-211 expression was upregulated in human non-small cell lung cancer (NSCLC) cell lines and tissues. The overexpression of miR-211 enhanced NSCLC cell proliferation, colony formation, and invasion. SRC kinase signaling inhibitor 1 (SRCIN1) was identified as a direct target of miR-211. SRCIN1 silencing promoted cell proliferation, and SRCIN1 expression was downregulated in human NSCLC cell lines. Thus, miR-211 may function as an oncogenic miRNA in NSCLC, partly by regulating SRCIN1, and the modulation of miR-211 expression represents a potential strategy for the treatment of NSCLC patients.

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Authors and Affiliations

  1. Department of Pulmonary Medicine, Harbin Medical University Cancer Hospital, Harbin, Heilongjiang, China, 150040

    Leiguang Ye, Hui Wang & Baogang Liu

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  1. Leiguang Ye
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  2. Hui Wang
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  3. Baogang Liu
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Correspondence to Baogang Liu.

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Leiguang Ye and Hui Wang are co-first authors

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Ye, L., Wang, H. & Liu, B. miR-211 promotes non-small cell lung cancer proliferation by targeting SRCIN1. Tumor Biol. 37, 1151–1157 (2016). https://doi.org/10.1007/s13277-015-3835-y

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