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Matrine inhibits the growth and induces apoptosis of osteosarcoma cells in vitro by inactivating the Akt pathway

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Tumor Biology

Abstract

Matrine, a natural product, has been demonstrated to be a promising chemotherapeutic drug for some cancers. Using flow cytometric analysis of the cell cycle and apoptosis, we found that matrine inhibited the proliferation and induced apoptosis in the human osteosarcoma (OS) cell lines MG63, HOS, U2OS, and SAOS2 in vitro in a dose-dependent manner. We therefore assessed the role of the serine/threonine kinase Akt in the regulation of matrine-mediated cell growth inhibition and apoptosis induction in human OS cell lines. After treatment for 48 h, matrine induced G0/G1-stage cell cycle arrest in MG63, U2OS, and SAOS2 cells associated with an increase in the expression of p27Kip1 and a decrease in the expression of Akt, glycogen synthase kinase 3 (GSK3)-β (Ser9), and cyclin D1. Furthermore, the pro-apoptotic factor Bax was upregulated. Overall, our findings suggest that matrine may be an effective anti-osteosarcoma drug due to its ability to inhibit proliferation and induce apoptosis in OS cells, possibly through the involvement of Akt signaling.

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Acknowledgments

We thank Dr. Hua Chen and Prof. Shang-Ha Pan (The First Affiliated Hospital of Harbin Medical University) for support with experiments. This study was supported by the National Natural Scinece Foundation of China (Grant No.81441081) and the Science and Technology Research Foundation of Heilongjiang Provincial Education Department (Grant No. 12521256).

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Authors and Affiliations

  1. Department of Orthopedics, The Second Affiliated Hospital of Harbin Medical University, 148 Baojian Road, Harbin, 150081, China

    Gong-** Xu, Wei Zhao, **-Peng Zhuang, Jia-Ning Zu, Duan-Yang Wang, Fei Han, Zhi-Peng Zhang & **g-Long Yan

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  1. Gong-** Xu
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  2. Wei Zhao
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  7. Zhi-Peng Zhang
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Correspondence to Zhi-Peng Zhang or **g-Long Yan.

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Gong-** Xu and Wei Zhao contributed equally to this work.

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Xu, GP., Zhao, W., Zhuang, JP. et al. Matrine inhibits the growth and induces apoptosis of osteosarcoma cells in vitro by inactivating the Akt pathway. Tumor Biol. 36, 1653–1659 (2015). https://doi.org/10.1007/s13277-014-2764-5

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  • DOI: https://doi.org/10.1007/s13277-014-2764-5

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