Abstract
The combination of therapeutic materials and biocompatible scaffolds to create multifunctional scaffolds with therapeutic and restorative functions has been investigated as a new therapeutic modality for bone cancers because the integration of multi-modal therapies into one platform can exhibit significant potential in overcoming the drawbacks of conventional therapy in bone cancers. In this study, we develop an IR780- and epigallocatechin gallate (EGCG)-loaded multifunctional polylactic acid (PLA) (E-PLMH780) composite scaffold for chemo-photothermal combination therapy without cytotoxicity. This bilayered E-PLMH780 scaffold is prepared using a layer of EGCG-loaded PLA monolith and a three-dimensionally printed PLA composite scaffold, followed by coating with IR780-loaded Mg-HA nanoparticles. The monolith layer in the composite scaffold exhibits a three-dimensional porous structure and a uniform morphology featuring small leaf-like units, whereas the three-dimensionally printed layer exhibits a uniform porous structure with a hierarchical architecture. The results of the chemo-photothermal combination therapy and cytotoxicity assays showed that the E-PLMH780 scaffold effectively inhibited bone cancer cell proliferation but did not cause cytotoxicity in normal osteoblastic cells. Therefore, chemo-photothermal combination therapy using IR780- and EGCG-loaded E-PLMH780 scaffolds can potentially provide a new generation of therapeutic modality for the improved treatment of bone cancers and repair of bone defects.
Graphical abstract
Schematic diagram of the fabrication of IR780- and EGCG-loaded E-PLMH780 scaffolds and their therapeutic efficacy on cancer cells
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Acknowledgements
This research was partially supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2020R1I1A3061425) and by the Ministry of Trade, Industry & Energy (MOTIE) for a study on “Development of beta Ti alloys wrought materials and applied parts for consumer goods (No. 20016574), Republic of Korea.
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Kim, J.W., Kwon, J.H., Kim, K.H. et al. Bilayered porous composite scaffolds for enhanced treatment of bone cancer through chemo-photothermal combination therapy. Macromol. Res. (2024). https://doi.org/10.1007/s13233-024-00256-8
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DOI: https://doi.org/10.1007/s13233-024-00256-8