Abstract
Inflammatory bowel disease (IBD) is a group of disorders characterized by chronic inflammation in the intestine. Several studies confirmed that oxidative stress induced by an enormous amount of reactive free radicals triggers the onset of IBD. Currently, there is an increasing trend in the global incidence of IBD and it is coupled with a lack of adequate long-term therapeutic options. At the same time, progress in research to understand the pathogenesis of IBD has been hampered due to the absence of adequate animal models. Currently, the toxic chemical Dextran Sulfate Sodium (DSS) induced gut inflammation in rodents is widely perceived as a good model of experimental colitis or IBD. Drosophila melanogaster, a genetic animal model, shares ~ 75% sequence similarity to genes causing different diseases in humans and also has conserved digestion and absorption features. Therefore, in the current study, we used Drosophila as a model system to induce and investigate DSS-induced colitis. Anatomical, biochemical, and molecular analyses were performed to measure the levels of inflammation and cellular disturbances in the gastrointestinal (GI) tract of Drosophila. Our study shows that DSS-induced inflammation lowers the levels of antioxidant molecules, affects the life span, reduces physiological activity and induces cellular damage in the GI tract mimicking pathophysiological features of IBD in Drosophila. Such a DSS-induced Drosophila colitis model can be further used for understanding the molecular pathology of IBD and screening novel drugs.
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Acknowledgements
The authors thank the DST-PURSE facility, Mangalore University, for the confocal microscopy. Dr. Shamprasad Varija Raghu is grateful to DBT Ramalingaswami Fellowship. The authors thank UGC-SAP Funded Facility at the Department of Applied Zoology, Mangalore University for qPCR analysis and Dr. Anurag Sharma, NUCSER, Nitte (Deemed to be University), Mangalore for providing the upd2 and upd3 primers.
Funding
SVR is grateful to the Department of Biotechnology (DBT), Government of India for financial support as DBT-Ramalingaswami Re-entry fellowship (102/IFD/SAN/1998/2015-16).
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The current study does not involve any human participants. The use of wild type Drosophila melanogaster in the current studies is approved by the Institutional Ethical committee (MU/AZ/1091/IAEC/2016-17).
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: qPCR analysis for the gut microbiota (bacterial phyla specific analysis) using the digestive tract of control and DSS-treated flies. All the assays were performed in triplicates (TIF 3843 KB)
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Keshav, N., Ammankallu, R., Shashidhar et al. Dextran sodium sulfate alters antioxidant status in the gut affecting the survival of Drosophila melanogaster. 3 Biotech 12, 280 (2022). https://doi.org/10.1007/s13205-022-03349-2
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DOI: https://doi.org/10.1007/s13205-022-03349-2