Abstract
Plant UDP-glucose 6-dehydrogenase (UGDH) is an important enzyme for the formation of hemicellulose and pectin. Previous studies on UGDH have primarily focused on the biosynthesis of cell wall polysaccharides, while few studies have focused on their regulation by exogenous nitrogen. In the present study, four genes encoding PtUGDH proteins were analyzed by bioinformatics methods. And, the expression profiles of PtUGDH genes under different nitrogen treatments were evaluated with qRT-PCR. The results showed that PtUGDHs have conserved NAD coenzyme binding motif GAGYVGG and the catalytic motif GFGGSCFQKDIL. According to the phylogenetic analysis, PtUGDHs were divided into two subgroups. PtUGDH3 and PtUGDH4 were closely related to AtUGDH1 (important for normal development of Arabidopsis cell wall structure). Chromosomal distribution and genome synteny analysis revealed four segmental-duplicated gene pairs on chr4, 8, 10 and 17. Tissue-specific data from PlantGenIE showed that PtUGDH3 and PtUGDH4 were highly expressed in stems. The qRT-PCR detection showed that the expression of PtUGDH3 in the lower stem and PtUGDH2 of upper leaves were significantly increased induced by low ammonium or nitrate condition. This comprehensive analysis of the UGDH family in poplar provides new insights into their regulation by nitrogen, and would increase our understanding of the roles of UGDHs in hemicellulose and pectin biosynthesis in the cell wall and during poplar development.
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Acknowledgements
This work was supported by the Special Fund for Basic Scientific research operation Fee of Central University (2572017EA05), Science Fund Project of Heilongjiang Province of China (LH2020C043), and the Innovation Project of State Key Laboratory of Tree Genetics and Breeding (Northeast Forestry University) (2019A03).
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Z.X. and C.Q. conceived and designed the study, S.Z. performed most of the experiments, J.Y., X.S. and L.C. conducted the sampling, R.C., and X.X. performed bioinformatics calculations, J.S. and G.L. processed and analyzed the data, and S.Z., C.Q., and Z.X. wrote the manuscript.
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Supplementary Information
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13205_2021_2697_MOESM4_ESM.docx
Supplementary file4 (DOCX 15 KB) Table S4 Tissue-specific expression data of roots, internodes, nodes, mature leaves and young leaves derived from PlantGenIE online tool.
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Supplementary file5 (DOCX 19 KB) Table S5 Effect of nitrogen on transcript levels of PtUGDHs in different tissues by qRT-PCRs.
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Supplementary file7 (TIF 53 KB) Fig S2 Structure of the corresponding UGDH genes in Arabidopsis. CDSs and the upstream/downstream sequences were represented by yellow and blue lines, respectively.
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Supplementary file8 (TIF 27 KB) Fig S3 Chromosomal distribution of PtUGDHs. The chromosome number and the gene name were written on the upper and left side of the red line, respectively. The red line represents the chromosome, and the leftmost scale indicates the chromosome length (Mb).
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Zhang, S., Cao, L., Sun, X. et al. Genome-wide analysis of UGDH genes in Populus trichocarpa and responsiveness to nitrogen treatment. 3 Biotech 11, 149 (2021). https://doi.org/10.1007/s13205-021-02697-9
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DOI: https://doi.org/10.1007/s13205-021-02697-9