Abstract
Sources of resistance to powdery mildew incited by Golovinomyces orontii have been identified in wild sunflowers and few exotic lines. The present investigation has been undertaken to study the inheritance of powdery mildew resistance and to map the quantitative trait loci (QTLs) governing resistance to powdery mildew in a multiple disease resistance line, TX16R (PI 642072). The inheritance was observed as a continuous distribution in a set of 264 F2 population and 93 recombinant inbred lines (RILs) of a cross between a highly susceptible accession PS 2023 and TX16R. Screening of the two population sets was done with 484 sunflower-specific SSR primers of which 175 primers showed polymorphism between the parents. Based on the phenoty** and genoty** data, the linkage map was constructed with 93 RILs. The map spanned 1200 cM and included 64 markers distributed along the 17 sunflower chromosomes in the haploid set. Quantitative trait loci (QTL) analysis identified three genomic regions for resistance to powdery mildew, two of which mapped on chromosome 10 and one on chromosome 5. This is the first report on map** of powdery mildew resistance in sunflower and paves the way in fine map** and introgression of resistance for powdery mildew in sunflower through marker-assisted breeding.
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The Junior Research Fellowship to PRK for carrying out this research was funded by University Grants Commission, New Delhi, India.
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13205_2020_2224_MOESM1_ESM.tif
Supplementary Figure 1 Descriptive pictures representing the 0-9 scale of powdery mildew based on area of leaf infected as well as spread of powdery mildew on plants. With regard to disease infection level, the plant material was scored as 0= no infection on leaves; low (L) - if leaves had < 10% infection; moderate (M) - if leaves had 11-30% infection, and high (H) - if leaves had 31-100% infection (TIF 13150 kb)
13205_2020_2224_MOESM2_ESM.tif
Supplementary Figure 2 The pictures of susceptible (PS 2023) and resistant (TX16R) parents showing powdery mildew symptoms (Suppl. Figures 2a and 2b, respectively). Also, pictures of infected plants of F2 (Suppl. Figures 2c, 2d, and 2e) and RIL populations (Suppl. Figures 2f, 2g) showing different disease severity grades from 0 to 9 (TIF 40510 kb)
13205_2020_2224_MOESM3_ESM.tif
Supplementary Figure 3 Gel image of the markers showing polymorphism between the parents PS 2023 (susceptible) and TX16R (resistant) (a), Gel images of the SSR marker ORS 510 and ORS 1288 (b) markers assayed in the 93 RIL population. Black arrows indicate polymorphism (TIF 19531 kb)
13205_2020_2224_MOESM4_ESM.docx
Supplementary table 1 List of polymorphic primers used in genoty** the RIL population derived from the cross between PS 2023 and TX16R (DOCX 21 kb)
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Kallamadi, P.R., Mulpuri, S. Inheritance and molecular map** of powdery mildew (Golovinomyces orontii) resistance gene(s) in sunflower (Helianthus annuus L.). 3 Biotech 10, 234 (2020). https://doi.org/10.1007/s13205-020-02224-2
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DOI: https://doi.org/10.1007/s13205-020-02224-2