SpringerLink
Log in

Free and immobilized Aspergillus oryzae SBS50 producing protease-resistant and thermostable phytase

  • Original Article
  • Published:
3 Biotech Aims and scope Submit manuscript

Abstract

Optimization for enhanced phytase production by Aspergillus oryzae SBS50 in submerged fermentation was investigated using Taguchi design. In first step design, starch, beef extract, magnesium sulphate, ferrous sulphate and Tween 80 were identified as significant factors affecting phytase production. These significant factors were further optimized at four different levels using a second Taguchi design and were observed that 1% starch, 2% beef extact, 3% Tween 80, 0.1% magnesium sulphate and 0.225% ferrous sulphate supported maximum phytase production (47,432 U/L). The use of Taguchi designed experiments resulted in 14.9-fold enhancement in phytase production compared to the medium optimized by ‘one variable at a time’ approach. Furthermore, 4% agar immobilized conidiospores of A. oryzae supported high phytase production compared with free cells and other matrices. Agar-immobilized conidiospores resulted in sustained phytase production up to eight repeated batch cycles followed by a decrease in enzyme titres.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price includes VAT (Germany)

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Assemi H, Rezapanah M, Vafaei-Shoushtari R, Mehrvar A (2012) Modified artificial diet for rearing of tobacco budworm, Helicoverpa armigera, using the Taguchi method and Derringer’s desirability function. J Insect Sci 12:100

    Article  CAS  Google Scholar 

  • Awad GEA, Esawy AM, El-Gammal EW, Ahmed HM, Elnashar MM, Atwa NA (2015) Comparative studies of free and immobilized phytase, produced by Penicillium purpurogenu GE1, using grafted alginate/carrageenan beads. Egypt Pharm J 14:87–93

    Article  Google Scholar 

  • Bajaj K, Wani MA (2011) Enhanced phytase production from Nocardia sp. MB 36 using agro-residues as substrates: potential application for animal feed production. Eng Life Sci 11(6):620–628

    Article  CAS  Google Scholar 

  • Bajaj K, Wani MA (2015) Purification and characterization of a novel phytase from Nocardia sp. MB 36. Biocatal Biotransform 33:141–149

    Article  CAS  Google Scholar 

  • Bhavsar K, Kumar VR, Khire JM (2012) Downstream processing of extracellular phytase from Aspergillus niger: chromatography process vs. aqueous two phase extraction for its simultaneous partitioning and purification. Process Biochem 47(7):1066–1072

    Article  CAS  Google Scholar 

  • Bucke C (1987) Cell immobilization in calcium alginate. In: Mosbach K (ed) Methods in ezymology, vol 135B. Academic press, New York, pp 175–189

    Google Scholar 

  • Chadha BS, Gulati H, Minhas M, Saini HS, Singh N (2004) Phytase production by the thermophilic fungus Rhizomucor pusillus. World J Microbiol Biotechnol 20:105–109

    Article  CAS  Google Scholar 

  • Coban HB, Demisa A (2014) Screening of phytase producers and optimization of culture conditions for submerged fermentations. Bioprocess Biosyst Eng 37(4):609–616

    Article  CAS  Google Scholar 

  • Das S, Ghosh U (2014) Effect of nutritional supplementation of solid state fermentation medium on biosynthesis of phytase fom Aspergillus niger NCIM 612. J Sci Indust Res 73:593–597

    CAS  Google Scholar 

  • Fiske CH, Subbarow YP (1925) The colorimetric determination of phosphorus. J Biol Chem 66:375–400

    CAS  Google Scholar 

  • Hemachander C, Boss N, Puvanakrishnan R (2001) Whole cell immobilization of Ralstonia pickettii for lipase production. Process Biochem 36(7):629–633

    Article  CAS  Google Scholar 

  • Heravi KM, Eftekhar F, Yakhchali B, Tabandeh F (2008) Isolation and identification of a lipase producing Bacillus sp. from soil. Pak J Biol Sci 11(5):740–745

    Article  CAS  Google Scholar 

  • Jain J, Sapna, Singh B (2016) Characteristics and biotechnological applications of bacterial phytases. Process Biochem 51:159–169

    Article  CAS  Google Scholar 

  • Jalasutram V, Jetty A (2011) Optimization of 1, 3-Propanediol production by Klebsiella pneumoniae 141B using Taguchi methodology: improvement in production by cofermentation studies. Res Biotechnol 2(2):90–104

    Google Scholar 

  • Kaur P, Satyanarayana T (2010) Improvement in cell-bound phytase activity of Pichia anomala by permeabilization and applicability of permeabilized cells in soymilk dephytinization. J Appl Microbiol 108(6):2041–2049

    CAS  Google Scholar 

  • Kuek C (1991) Production of glucoamylase using Aspergillus phoenicus immobilized in calcium alginate beads. Appl Microbiol Biotechnol 35:466–470

    Article  CAS  Google Scholar 

  • Kumar P, Satyanarayana T (2007) Optimization of culture variables for improving glucoamylase production by alginate-entrapped Thermomucor indicae-seudaticae using statistical methods. Bioresour Technol 98:1252–1259

    Article  CAS  Google Scholar 

  • Kumar V, Yadav AN, Verma P, Sangwan P, Saxena A, Kumar K, Singh B (2017) β-Propeller phytases: diversity, catalytic attributes, biotechnological improvements and applications. Int J Biol Macromol 98:595–609

    Article  CAS  Google Scholar 

  • Kumari A, Singh B, Satyanarayana T (2016) Mixed substrate fermentation for enhanced phytase production by thermophilic mould Sporotrichum thermophile and its application in beneficiation of poultry feed. Appl Biochem Biotechnol 178:197–210

    Article  CAS  Google Scholar 

  • Maller A, Vici CA, Facchini FDA, da Silva TM, Kamimura ES, Rodrigues MI, Jorge JA, Terenzi HF, Polizeli MLTM (2014) Increase of the phytase production by Aspergillus japonicus and its biocatalyst on chicken feed treatment. J Basic Microbiol 54:S152–S160

    Article  CAS  Google Scholar 

  • Park JK, Chang HN (2000) Microencapsulation of microbial cells. Biotechnol Adv 18(4):303–319

    Article  CAS  Google Scholar 

  • Quan CS, Fan SD, Ohta Y (2003) Immobilization of Candida krusei cells producing phytase in alginate gel beads: an application of the preparation of myo-inositol phosphates. Appl Microbiol Biotechnol 62:41–47

    Article  CAS  Google Scholar 

  • Sedghi M, Golian A, Esmaeilipour O, Van Krimpen MM (2014) Application of the Taguchi method in poultry science: estimation of the in vitro optimum intrinsic phytase activity of rye, wheat and barley. British Poul Sci 55(2):246–252

    Article  CAS  Google Scholar 

  • Sapna, Singh B (2013) Improved production of protease-resisitant phytase by Aspergillus oryzae and its applicability in the hydrolysis of insoluble phytates. J Indust Microbiol Biotechnol 40(8):891–899

    Article  CAS  Google Scholar 

  • Sapna, Singh B (2014) Phytase production by Aspergillus oryzae in solid state fermentation and its applicability in dephytinization of wheat bran. Appl Biochem Biotechnol 173(7):1885–1895

    Article  CAS  Google Scholar 

  • Sapna, Singh B (2015) Biocatalytic potential of protease-resistant phytase of Aspergillus oryzae SBS50 in ameliorating food nutrition. Biocatal Biotransfom 33(3):167–174

    Article  CAS  Google Scholar 

  • Singh B, Satyanarayana T (2006) A marked enhancement in phytase production by a thermophilic mould Sporotrichum thermophile using statistical designs in a cost-effective cane molasses medium. J Appl Microbiol 101:344–352

    Article  CAS  Google Scholar 

  • Singh B, Satyanarayana T (2008) Phytase production by Sporotrichum thermophile in a cost-effective cane molasses medium in submerged fermentation and its application in bread. J Appl Microbiol 105:1858–1865

    Article  CAS  Google Scholar 

  • Singh B, Satyanarayana T (2015) Fungal phytases: characteristics and amelioration of nutritional quality and growth of non ruminants. J Anim Physiol Nutr 99:646–660

    Article  CAS  Google Scholar 

  • Singh B, Kunze G, Satyanarayana T (2011) Developments in biochemical aspects and biotechnological applications of microbial phytases. Biotechnol Mol Biol Rev 6(3):69–87

    CAS  Google Scholar 

  • Vats P, Banerjee UC (2004) Production studies and catalytic properties of phytases (myo-inositolhexakisphosphate phosphohydrolases): an overview. Enzyme Microbial Technol 35:3–14

    Article  CAS  Google Scholar 

  • Vohra A, Satyanarayana T (2002) Statistical optimization of the medium components by response surface methodology to enhance phytase production by Pichia anomala. Process Biochem 37:999–1004

    Article  CAS  Google Scholar 

Download references

Acknowledgements

The authors acknowledge the financial assistance from University Grants Commission [F. No. 39-203/2010 (SR)], New Delhi during the course of this investigation.

Author information

Authors and Affiliations

  1. Laboratory of Bioprocess Technology, Department of Microbiology, Maharshi Dayanand University, Rohtak, Haryana, 124001, India

    Sapna & Bijender Singh

Authors
  1. Sapna
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Bijender Singh
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Bijender Singh.

Ethics declarations

Conflict of interest

All the authors declare that they have no conflict of interest.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Sapna, Singh, B. Free and immobilized Aspergillus oryzae SBS50 producing protease-resistant and thermostable phytase. 3 Biotech 7, 213 (2017). https://doi.org/10.1007/s13205-017-0804-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s13205-017-0804-8

Keywords

Search

Navigation