Abstract
Three new species belonging to the butterflies satyrine subtribe Euptychiina are described: (1) Yphthimoides gabriela n. sp. occurring in low to medium altitudes in the coastal forests between the states of Rio de Janeiro and Bahia, (2) Yphthimoides bella n. sp., from Brazilian Cerrado savannas, known from only two localities in the State of Goiás, and (3) Yphthimoides iserhardi n. sp., occurring in high altitudes in the rocky outcrops of Chapada Diamantina, in the interior of the Bahia state. Descriptions are based on wing shape, wing pattern, and morphology of male genitalia. Furthermore, molecular data from the “DNA barcode” (Cytochrome C Oxidase I, ca. 658 bp) was obtained and used to validate the three new species. Additionally, information about geographic distribution and habitat for the three new species is provided, and the systematic position of each of the three species is discussed based on a molecular analysis using another 11 additional species of Yphthimoides.
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1007%2Fs13127-015-0221-y/MediaObjects/13127_2015_221_Fig1_HTML.gif)
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1007%2Fs13127-015-0221-y/MediaObjects/13127_2015_221_Fig2_HTML.gif)
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1007%2Fs13127-015-0221-y/MediaObjects/13127_2015_221_Fig3_HTML.gif)
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1007%2Fs13127-015-0221-y/MediaObjects/13127_2015_221_Fig4_HTML.gif)
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1007%2Fs13127-015-0221-y/MediaObjects/13127_2015_221_Fig5_HTML.gif)
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1007%2Fs13127-015-0221-y/MediaObjects/13127_2015_221_Fig6_HTML.gif)
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1007%2Fs13127-015-0221-y/MediaObjects/13127_2015_221_Fig7_HTML.gif)
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1007%2Fs13127-015-0221-y/MediaObjects/13127_2015_221_Fig8_HTML.gif)
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1007%2Fs13127-015-0221-y/MediaObjects/13127_2015_221_Fig9_HTML.gif)
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1007%2Fs13127-015-0221-y/MediaObjects/13127_2015_221_Fig10_HTML.gif)
Similar content being viewed by others
References
Dayrat, B. (2005). Towards integrative taxonomy. Biological Journal of the Linnean Society, 85, 407–415.
Folmer, O., Black, M., Hoeh, W., Lutz, R., & Vrijenhoek, R. (1994). DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 3, 294–299.
Forster, J. R. (1964). Beiträge zur Kenntnis der Insektenfauna Boliviens XIX. Lepidoptera III. Satyridae. Veröffentlichungen der zoologischen Staatssammlung München, 8, 51–188. pls. 27–35.
Freitas, A. V. L. (2004). A new species of Yphthimoides (Nymphalidae, Satyrinae) from southeastern Brazil. Journal of the Lepidopterists’ Society, 58(1), 7–12.
Freitas, A. V. L. (2007). A new species of Moneuptychia Forster (Lepidoptera: Satyrinae, Euptychiina) from the highlands of southeastern Brazil. Neotropical Entomology, 36(6), 919–925. doi:10.1590/s1519-566x2007000600014.
Freitas, A. V. L., Kaminski, L. A., Mielke, O. H. H., Barbosa, E. P., & Silva-Brandão, K. L. (2012). A new species of Yphthimoides (Lepidoptera: Nymphalidae: Satyrinae) from the southern Atlantic forest region. Zootaxa, 3526, 31–44.
Freitas, A. V. L., Barbosa, E. P., Santos, J. P., & Mielke, O. H. H. (2013). A new genus, Atlanteuptychia gen. nov., for Euptychia ernestina (Lepidoptera: Nymphalidae: Satyrinae). Zoologia (Curitiba), 30(6), 661–668.
Hall, T. (2013). Biological sequence alignment editor software. Carlsbad: Ibis Biosciences. 92008.
Huertas, B., Ríos, C., & Le Crom, J. F. (2009). A new species of Splendeuptychia from the Magdalena Valley in Colombia (Lepidoptera: Nymphalidae: Satyrinae). Zootaxa, 2014, 51–58.
Kimura, M. (1980). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution, 16, 111–120.
Lamas, G. (2004). Nymphalidae. Satyrinae. Subtribe Euptychiina. In G. Lamas (Ed.), Checklist: Part 4A. Hesperioidea - Papilionoidea. In J. B. Heppner (Ed.) Atlas of Neotropical Lepidoptera. Gainesville: Association for Tropical Lepidoptera/Scientific Publishers, 428 pp
Marín, M., López, A., Freitas, A. V. L., & Uribe, S. I. (2009). Caracterización molecular de Euptychiina (Lepidoptera: Satyrinae) del norte de la cordillera central de los Andes. Revista Colombiana de Entomologia, 35, 235–244.
Marín, M. A., Peña, C., Freitas, A. V. L., Wahlberg, N., & Uribe, S. I. (2011). From the phylogeny of the Satyrinae butterflies to the systematics of Euptychiina (Lepidoptera: Nymphalidae): history, progress and prospects. Neotropical Entomology, 40(1), 1–13. doi:10.1590/s1519-566x2011000100001.
Matos-Maraví, P. F., Peña, C., Willmott, K. R., Freitas, A. V. L., & Wahlberg, N. (2013). Systematics and evolutionary history of butterflies in the “Taygetis clade” (Nymphalidae: Satyrinae: Euptychiina): towards a better understanding of Neotropical biogeography. Molecular Phylogenetics and Evolution, 66, 54–68. doi:10.1016/j.ympev.2012.09.005.
Miller, M. A., Pfeiffer, W., & Schwartz, T. (2010). "Creating the CIPRES Science Gateway for inference of large phylogenetic trees" in Proceedings of the Gateway Computing Environments Workshop (GCE), 14 Nov. 2010, New Orleans, LA pp 1–8.
Murray, D., & Prowell, D. P. (2005). Molecular phylogenetics and evolutionary history of the neotropical satyrine subtribe Euptychiina (Nymphalidae: Satyrinae). Molecular Phylogenetics and Evolution, 34, 67–80.
Pante, E., Schoelinck, C., & Puillandre, N. (2015). From integrative taxonomy to species description: one step beyond. Systematic Biology, 64(1), 152–160.
Peña, C., Wahlberg, N., Weingartner, E., Kodandaramaiah, U., Nylin, S., Freitas, A. V. L., & Brower, A. V. Z. (2006). Higher level phylogeny of Satyrinae butterflies (Lepidoptera: Nymphalidae) based on DNA sequence data. Molecular Phylogenetics and Evolution, 40, 29–49.
Peña, C., Nylin, S., Freitas, A. V. L., & Wahlberg, N. (2010). Biogeographic history of the butterfly subtribe Euptychiina (Lepidoptera, Nymphalidae, Satyrinae). Zoologica Scripta, 39, 243–258.
Peña, C., Nylin, S., & Wahlberg, N. (2011). The radiation of Satyrini butterflies (Nymphalidae: Satyrinae): a challenge for phylogenetic methods. Zoological Journal of the Linnean Society, 161, 64–87.
Pulido, H. W., & Andrade, M. G. (2008). A new species of Forsterinaria Gray, 1973 (Lepidoptera: Nymphalidae: Satyrinae) from the Serranía del Perijá, Cesar, Colombia. Caldasia, 30, 189–195.
Seraphim, N., Marín, M. A., Freitas, A. V. L., & Silva-Brandão, K. L. (2014). Morphological and molecular marker contributions to disentangling the cryptic Hermeuptychia hermes species complex (Nymphalidae: Satyrinae: Euptychiina). Molecular Ecology Resources, 14, 39–49.
Siewert, R. R., Zacca, T., Dias, F. M. S., Freitas, A. V. L., Mielke, O. H. H., & Casagrande, M. M. (2013). The “Taygetis ypthima species group” (Lepidoptera, Nymphalidae, Satyrinae): taxonomy, variation and description of a new species. ZooKeys, 356, 11–29. doi:10.3897/zookeys.356.6481.
Silva-Brandão, K. L., Freitas, A. V. L., Brower, A. V. Z., & Solferini, V. N. (2005). Phylogenetic relationships of the New World Troidini swallowtails (Lepidoptera: Papilionidae) based on COI, COII, and EF-1 alpha genes. Molecular Phylogenetics and Evolution, 36, 468–483.
Stamatakis, A., Hoover, P., & Rougemont, J. (2008). A rapid bootstrap algorithm for the RAxML web-servers. Systematic Biology, 75, 758–771.
Tamura, K., Stecher, G., Peterson, D., Filipski, A., & Kumar, S. (2013). MEGA6: molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution, 30, 2725–2729.
Wahlberg, N., Leneveu, J., Kodandaramaiah, U., Peña, C., Nylin, S., Freitas, A. V. L., & Brower, A. V. Z. (2009). Nymphalid butterflies diversify following near demise at the Cretaceous/Tertiary boundary. Proceedings of the Royal Society Series B, 276, 4295–4302.
Warren, A., D., Davis, K., J., Stangeland, E., M., Pelham, J., P., & Grishin, N., V., (2013). Illustrated lists of American butterflies. Available from: http://www.butterfliesofamerica.com/ 23 April 2015.
Yeates, D. K., Seago, A., Nelson, L., Cameron, S. L., Joseph, L., & Trueman, J. W. H. (2011). Integrative taxonomy, or iterative taxonomy? Systematic Entomology, 36, 209–217.
Zacca, T., Mielke, O. H. H., Pyrcz, T. W., Casagrande, M. M., Freitas, A. V. L., & Boyer, P. (2013). Stegosatyrus, a new genus of Euptychiina from the grasslands of neotropical realm (Lepidoptera: Nymphalidae: Satyrinae). Zootaxa, 3682(2), 331–350.
Zacca, T., Siewert, R. R., Mielke, O. H. H., & Casagrande, M. M. (2014). A new species of Magneuptychia Forster, 1964 (Lepidoptera: Nymphalidae: Satyrinae) from Brazilian Savanna. Zootaxa, 3795(1), 71–78.
Acknowledgments
The authors would like to thank Keith Willmott and two anonymous reviewers for critically reading the manuscript. Thanks also to Blanca Huertas, Thamara Zacca, Ricardo Siewert, Gláucia Marconato, and Andreas Segerer for hel** with the data from the material deposited at the BMNH, at the DZUP, and at the ZSM. Lucas Kaminski revised and made comments on the first drafts of the manuscript. The following people helped sending us material: Elaine C. Barbosa and Rodrigo N. Vasconcelos (Y. gabriela), Marcio Z. Cardoso and Paulo Enrique C. Peixoto (Y. iserhardi), and Lucas A. Kaminski and Junia Yasmin Oliveira Carrera (Y. bella). EPB acknowledges FAPESP (2012/03750-8) for a graduate fellowship. AKS thanks CAPES for a fellowship. AVLF thanks the Brazilian Research Council—CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico) (fellowship 302585/2011-7), and the National Science Foundation (DEB-1256742). This publication is part of the following projects: (1) RedeLep “Rede Nacional de Pesquisa e Conservação de Lepidópteros” SISBIOTA-Brasil/CNPq (563332/2010-7); (2) “Identificação Molecular de Biodiversidade de Invertebrados Terrestres” (Grant 564954/2010-1) included in the “Rede Nacional de Identificação Molecular da Biodiversidade—BR-BoL” (MCT/CNPq/FNDCT 50/2010); (3) BIOTA-FAPESP Program (2011/50225-3); and (4) the collaborative grant “Dimensions US-BIOTA São Paulo: A multidisciplinary framework for biodiversity prediction in the Brazilian Atlantic forest hotspot”, US NSF, NASA, and FAPESP (grant 2013/50297-0).
Ethical standards
The research contained within this manuscript is in accordance with Brazilian laws.
Conflicts of interest
The authors declare that they have no conflict of interest.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Barbosa, E.P., Silva, A.K., Paluch, M. et al. Uncovering the hidden diversity of the Neotropical butterfly genus Yphthimoides Forster (Nymphalidae: Satyrinae): description of three new species based on morphological and molecular data. Org Divers Evol 15, 577–589 (2015). https://doi.org/10.1007/s13127-015-0221-y
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s13127-015-0221-y