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Identification and characterization of SNPs in released, landrace and wild accessions of mungbean (Vigna radiata (L.) Wilczek) using whole genome re-sequencing

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Abstract

Mungbean [Vigna radiata (L.) R. Wilczek var. radiata] is vital grain legume having nutritional and socio-economic importance, especially in the develo** countries. We performed whole-genome re-sequencing of three accessions representing the wild progenitor species, released and landrace of mungbean to identify SNPs with relevance to genetic relationships analysis. Approximately 9.3 million raw reads were obtained using Ion Torrent PGM™ platform and more than 92% of the reads were mapped to the reference mungbean genome. We identified a total of 233,799 single-nucleotide polymorphisms in relation to the reference genome (SNPs: 103,341 in wild, 93,078 in released and 37,380 in landrace accessions) and 9544 insertions and deletions (InDels: 4742 in wild, 3608 in released and 1194 in landrace accessions) in the coding and non-coding regions. In all accessions, genomic variants were unevenly distributed within and across the mungbean chromosomes. Among these 5339, 4739 and 1795 SNPs were non-synonymous in 815, 790 and 317 genes of wild, released and landrace accessions, respectively. These polymorphisms might contribute to the variation in important pathways of genes for abiotic and biotic stress tolerance and important agronomic traits, such as seed dormancy, flowering time and seed size in mungbean. Among the randomly selected SNPs, a selected subset was validated using Sanger sequencing technique. The genomic variations among mungbean wild, released and landrace accessions constitute a powerful tool to support genetic research and molecular breeding of mungbean.

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Acknowledgements

The authors thank the Indian Council of Agricultural Research, New Delhi and, Director, National Bureau of Plant Genetic Resources for the facilities provided for the study. The first author, PB acknowledges the DBT Fellowship from The Department of Biotechnology, Ministry of Science & Technology, Government of India, New Delhi for the Ph. D program. The funding for the wet laboratory analysis from CRP on Genomics project of the Indian Council of Agricultural Research, New Delhi is gratefully acknowledged.

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Authors and Affiliations

  1. Genomic Resources Division, ICAR-National Bureau of Plant Genetic Resources, Pusa Campus, New Delhi, 110012, India

    Pooja Bangar, Neetu Tyagi, Bhavana Tiwari, Sanjay Kumar, Paramananda Barman, Ratna Kumari, Ambika Gaikwad & K. V. Bhat

  2. Department of Bio and Nano Technology, Bio and Nano Technology Centre, Guru Jambheshwar University of Science and Technology, Hisar, Haryana, 125001, India

    Pooja Bangar & Ashok Chaudhury

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  1. Pooja Bangar
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Bangar, P., Tyagi, N., Tiwari, B. et al. Identification and characterization of SNPs in released, landrace and wild accessions of mungbean (Vigna radiata (L.) Wilczek) using whole genome re-sequencing. J. Crop Sci. Biotechnol. 24, 153–165 (2021). https://doi.org/10.1007/s12892-020-00067-0

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