Abstract
Three species of catfish, Silurus biwaensis, Silurus lithophilus, and Silurus asotus, are identified in Lake Biwa. Notably, S. biwaensis and S. lithophilus are endemic species in Lake Biwa; however, their detailed distribution and ecology almost remain unknown. In this study, we developed catfish species-specific primer probe sets based on their whole genome. Using these, we analyzed the water samples collected from three tanks where S. biwaensis, S. lithophilus, and S. asotus were experimentally reared, respectively, and established a new detection system after confirming each primer probe set to be effective. Finally, we conducted environmental DNA analysis for catfishes in Lake Biwa using field samples. Overall, our results showed that environmental DNA analysis is a useful monitoring method for confirming the presence of endemic catfishes in Lake Biwa.
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Data Availability
The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.
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Acknowledgements
We would like to thank Dr. Toshifumi Minamoto for the helpful guidance to the sampling methods and assistance in eDNA analysis.
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All authors contributed to the study conception and design. Material preparation and data collection were performed by Testuro Inamoto, Yuu Kishimoto, Hisashi Okuyama and Ryoichi Tabata. Data analysis and Tables 1, 2 and 3 were performed by Testuro Inamoto, Koichiro Nakano and Jun-ichi Takahashi. The first draft of the manuscript was written by Tetsuro Inamoto and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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Inamoto, T., Kishimoto, Y., Okuyama, H. et al. Development of a multiplex real time-PCR assay to estimate the distribution of the genus Silurus in Lake Biwa using environmental DNA. Conservation Genet Resour 16, 111–115 (2024). https://doi.org/10.1007/s12686-023-01340-4
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DOI: https://doi.org/10.1007/s12686-023-01340-4