Abstract
Intervertebral disc (IVD) degeneration (IDD) has become a global health issue; however, effective treatment remains undeveloped. Although the potential curative effect of resveratrol (Res) on IDD has been reported, the explosive release and rapid disappearance of Res in lesions seriously limit its use. In this study, Res was loaded into solid lipid nanoparticles (SLNs) by emulsification and cryogenic coagulation, and Res–SLNs/gelatin methacryloyl (GelMA) composite hydrogel scaffolds were designed by GelMA hydrogel encapsulation to improve the stability of therapeutic disc degeneration. In vitro studies demonstrated that Res–SLNs can inhibit nucleus pulposus (NP; major IVD cell) apoptosis by upregulating the expression of anabolic proteins. In vivo studies showed that the Res–SLNs/GelMA hydrogel scaffold improved the pinning-induced IDD model in rats and restored the stability of the IVD extracellular matrix (ECM). Our experiments consistently show that implantation of this scaffold can improve the inflammatory microenvironment, reduce the degeneration of NP cells, and reinforce the disc function repair effect. Therefore, the Res–SLNs/GelMA hydrogel scaffold has great application prospects for treating IDD.
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Funding
This study was supported by grants from the 512 Talents Development Project of Bengbu Medical College (by51202302), the Opening Project of Anhui Province Key Laboratory of Tissue Trans-plantation in Bengbu Medical College (AHTT2022A001), the Domestic Visiting and Training Program for Outstanding Young Backbone Teachers in High Schools (gxgnfx2022036), and the Scientific Research Foundation of Bengbu Medical College (2021bypd006).
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Ying Wang and Yidi Xu have equally contributed to the article. Conceptualization: Yingji Mao. Writing—original draft preparation: Ying Wang and Yidi Xu. Writing—review and editing: Yingji Mao. Data curation and methodology: Ying Wang, Yidi Xu, Shihui Zhang, and Yingji Mao. Funding acquisition: Yingji Mao. All authors read and approved the final manuscript.
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All rat feeding and experimental surgical operations were approved by the Animal Ethics Committee of the Bengbu Medical College following the NIH guidelines.
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Article Highlights
Intervertebral disc degeneration (IDD) has become a global health problem; however, effective treatment modalities are currently scarce. We designed a GelMA hydrogel as a carrier-encapsulated hydrogel scaffold for Res–SLNs and evaluated the role of this hydrogel (Res–SLNs/GelMA) in modulating the IL-1β-induced inflammatory microenvironment in vitro and restoring IVD function in vivo. Focusing on the treatment of IDD in the in vivo inflammatory environment brings a new therapeutic idea for IDD repair and a new application prospect for Res.
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Wang, Y., Xu, Y., Zhang, S. et al. A Stable Hydrogel Scaffold with Anti-Inflammatory Effects Treats Intervertebral Disc Degeneration. BioNanoSci. 13, 1150–1162 (2023). https://doi.org/10.1007/s12668-023-01150-w
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DOI: https://doi.org/10.1007/s12668-023-01150-w