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Preparation of Keratin-Metal Complexes Derived from Different Treatments of Chicken Feather Waste

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Abstract

The work presented in this paper focuses on the extraction of keratin from chicken feather waste by the following two methods: (1) alkaline hydrolysis or (2) the reduction by L-cysteine reducing agent; resulting in the extracted yields of 35% and 55%, respectively. The keratin samples produced from the two extraction methods possessed different physical and chemical properties including molecular weight, crystallinity index, thermal behavior, metal binding ability (iron, copper, silver and zinc), antimicrobial activity, and cytotoxicity. The alkaline hydrolysis method yielded keratin of a lower molecular weight, crystallinity index and thermal degradation than the reduction method by L-cysteine. However, their chemical identity was determined to be qualitatively similar as characterized by infrared spectroscopy. The keratin produced by alkaline hydrolysis give rise to a higher metal content adsorbability and thus subsequently led to greater antimicrobial activity with an acceptable value of cytotoxicity. Overall, the work presented a biomaterial from chicken waste with outstanding properties particularly using the alkaline hydrolysis extraction method, which can be applied in a variety of biomedical industries.

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Data Availability

The datasets generated and analyzed during the current study are not publicly available due to time limitations but are available from the corresponding author on reasonable request.

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Acknowledgements

This work was supported by the National Research Council of Thailand (NRCT). The authors would like to thank Ms. Pennapa Takam and Mr. Thanawat Inkham for their effort in laboratory works.

Funding

This work was supported by the National Research Council of Thailand (NRCT). Arunee Kongdee Aldred and Panwad Sillapawattana have received the research support from NRCT.

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Authors and Affiliations

  1. Program in Industrial Chemistry Innovation, Faculty of Science, Maejo University, Chiang Mai, Thailand

    Arunee Kongdee Aldred

  2. Thailand Institute of Scientific and Technological Research (TISTR), Techno Polis, Pathum Thani, Thailand

    Prapaipat Klungsupya

  3. Program in Biotechnology, Faculty of Science, Maejo University, Chiang Mai, Thailand

    Wasin Charerntantanakul

  4. Institute for Building Materials Research, RWTH Aachen University, Aachen, Germany

    Oliver Weichold

  5. Program in Environmental Technology, Faculty of Science, Maejo University, Chiang Mai, Thailand

    Panwad Sillapawattana

Authors
  1. Arunee Kongdee Aldred
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  2. Prapaipat Klungsupya
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  3. Wasin Charerntantanakul
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  4. Oliver Weichold
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  5. Panwad Sillapawattana
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Contributions

All authors contributed to the study conception and design. Arunee Kongdee Aldred: conceptualization, methodology, writing original draft, supervision. Prapaipat Klungsupya: methodology, validation. Wasin Charerntantanakul: methodology, writing-reviewing and editing. Oliver Weichlold: conceptualization, validation, writing-reviewing and editing. Panwad Sillapawattana: conceptualization, methodology, data collection and analysis, writing original draft.

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Correspondence to Panwad Sillapawattana.

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Kongdee Aldred, A., Klungsupya, P., Charerntantanakul, W. et al. Preparation of Keratin-Metal Complexes Derived from Different Treatments of Chicken Feather Waste. Waste Biomass Valor 15, 115–125 (2024). https://doi.org/10.1007/s12649-023-02154-z

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