Abstract
Renewable energy security for the future and better use of natural resources are key challenges that can be concurrently managed by a practical anaerobic co-digestion approach where substrates with a high carbon/nitrogen (C/N) ratio are combined with lower ones to create a balance of the nutrients during the production of methane. This study determined the optimal conditions for methane production from anaerobic co-digestion of cassava biomass (CB) and winery solid waste (WSW) using response surface methodology (RSM). A three-factor central composite design was used to set-up the anaerobic co-digestion experiments. The individual and interactive effects of temperature (25–45 °C), pH 6–8 and a range of substrate (CB/WSW) ratios (0–100) on the methane yield were explored. Optimisation using RSM showed a close fit between the predicted and experimental data as indicated by the coefficient of determination (R2) value of 0.9521. The RSM model predicted a maximum methane yield of 346.28 mL CH4/g VSadded for the optimal conditions of pH 7, temperature of 35 °C ± 0.5 and 70/30 ratio of CB/WSW. The verification experiment produced 396 mL CH4/g VSadded, 12.6% higher than the predicted value at the same conditions. Although, there was a gap between the predicted and actual yield, the significance of the variables in the analysis of variance (ANOVA) shows the model could be relevant to similar research. The substrate ratio at 70 CB:30 WSW was the most significant factor during methane production. The RSM model proved successful in the optimisation process of methane yield.
Graphic Abstract
![](http://media.springernature.com/lw685/springer-static/image/art%3A10.1007%2Fs12649-019-00801-y/MediaObjects/12649_2019_801_Figa_HTML.png)
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1007%2Fs12649-019-00801-y/MediaObjects/12649_2019_801_Fig1_HTML.png)
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1007%2Fs12649-019-00801-y/MediaObjects/12649_2019_801_Fig2_HTML.png)
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1007%2Fs12649-019-00801-y/MediaObjects/12649_2019_801_Fig3_HTML.png)
Similar content being viewed by others
Abbreviations
- CB:
-
Cassava biomass
- WSW:
-
Winery solid waste
- CCD:
-
Central composite design
- RSM:
-
Response surface methodology
- AD:
-
Anaerobic digestion
- CH4 :
-
Methane
- GHG:
-
Greenhouse gas
- UN:
-
United Nations
- C/N:
-
Carbon to nitrogen ratio
- ZD:
-
Zebra dung
- ARC:
-
Agricultural Research Council
- RSA:
-
Republic of South Africa
- ANOVA:
-
Analysis of variance
- TSs:
-
Total solids
- VSs:
-
Volatile solids
- TOC:
-
Total organic carbon
- MC:
-
Moisture content
- ICP:
-
Inductively coupled plasma
- NaOH:
-
Sodium hydroxide
- HCl:
-
Hydrochloric acid
- VFA:
-
Volatile fatty acid
- H2SO4 :
-
Sulphuric acid
References
Bessou, C., Ferchaud, F., Gabrielle, B., Mary, B.: Biofuels, greenhouse gases and climate change. A review. Agron. Sustain. Dev. 31, 1 (2011). https://doi.org/10.1051/Agro/2009039
Vasavan, A., de Goey, P., van Oijen, J.: Numerical study on the autoignition of biogas in moderate or intense low oxygen dilution nonpremixed combustion systems. Energy Fuels 32, 8768–8780 (2018). https://doi.org/10.1021/acs.energyfuels.8b01388
Balat, M., Balat, H.: Biogas as a renewable energy source—a review. Energy Sources A 41(14), 1280–1293 (2009)
Demuynck, M., Nyns, E.J., Palz, W.: Biogas Plants in Europe. A Practical Handbook. Reidel Publishing Company, Dordrecht (1984)
Abdeshahian, P., Lim, J.S., Ho, W.S., Hashim, H., Lee, C.T.: Potential of biogas production from farm animal waste in Malaysia. Renew. Sustain. Energy Rev. 60, 714–723 (2016)
Bundhoo, Z.M.A., Mauthoor, S., Mohee, R.: Potential of biogas production from biomass and waste materials in the Small Island Develo** State of Mauritius. Renew. Sustain. Energy Rev. 56, 1087–1100 (2016). https://doi.org/10.1016/j.rser.2015.12.026
Dobre, P., Nicolae, F., Matei, F.: Main factors affecting biogas production—an overview. Rom. Biotechnol. Lett. 19, 9283–9296 (2014)
Okudoh, V., Trois, C., Workneh, T.: The potential of cassava biomass as a feedstock for sustainable biogas production in South Africa. J. Energy Power Eng. 8, 836–843 (2014)
Hansupalak, N., Piromkraipak, P., Tamthirat, P., Manitsorasak, A., Sriroth, K., Tran, T.: Biogas reduces the carbon footprint of cassava starch: a comparative assessment with fuel oil. J. Clean. Prod. (2016). https://doi.org/10.1016/j.jclepro.2015.06.138
Department of Agriculture, Forestry and Fisheries (DAFF) Republic of South Africa: Cassava production guideline (2010). https://www.nda.agric.za/docs/Brochures/ProdGuideCassava.pdf. Accessed 6 Sept 2018
WOSA: World Statistics. https://www.wosa.co.za/The-Industry/Statistics/World-Statistics/. Accessed 26 Feb 2018
Zacharof, M.P.: Grape winery waste as feedstock for bioconversions: applying the biorefinery concept. Waste Biomass Valoriz. 8(4), 1011–1025 (2017). https://doi.org/10.1007/s12649-016-9674-2
Zhang, Q., Hu, J., Lee, D.J.: Biogas from anaerobic digestion processes: research updates. Renew. Energy 98, 108–119 (2016). https://doi.org/10.1016/j.renene.2016.02.029
Achina, S., Achinas, V., Gerrit, J.W.E.: A technological overview of biogas production from biowaste. Engineering 3, 299–307 (2017)
Weiland, P.: Biogas production: current state and perspectives. Appl. Microbiol. Biotechnol. 85, 849–860 (2010)
Okudoh, V., Trois, C., Workneh, T., Schmidt, S.: The potential of cassava biomass and applicable technologies for sustainable biogas production in South Africa: a review. Renew. Sustain. Energy Rev. 39, 1035–1052 (2014). https://doi.org/10.1016/j.rser.2014.07.142
Debabrata, B.: Introduction to energy from toxic organic waste for heat and power generation. In: Debabrata, B. (ed) Energy from Toxic Organic Waste for Heat and Power Generation, pp. 1–6. Woodhead Publishing (2019). https://doi.org/10.1016/B978-0-08-102528-4.00001-8
Ning, J., Zhou, M., Pan, X., Li, C., et al.: Simultaneous biogas and biogas slurry production from co-digestion of pig manure and corn straw: performance optimization and microbial community shift. Bioresour. Technol. 282, 37–47 (2019). https://doi.org/10.1016/j.biortech.2019.02.122
Sawatdeenarunat, C., Surendra, K.C., Takara, D., Oechsner, H., Khanal, S.K.: Bi anaerobic digestion of lignocellulosic biomass: challenges and opportunities. Bioresour. Technol. 178, 178–186 (2015)
Mkruqulwa, U.L., Okudoh, V.I., Oyekola, O.O.: Biomethane potential from co-digestion of cassava and winery waste in South Africa. In: Proceedings of the 9th International Conference on Advances in Science, Engineering, Technology and Waste Management (ASETWM-17), 27–28 November, Parys, South Africa, pp. 107–122 (2017)
Riaño, B., Molinuevo, B., García-González, M.C.: Potential for methane production from anaerobic co-digestion of swine manure with winery wastewater. Bioresour. Technol. 102, 4131–4136 (2011). https://doi.org/10.1016/j.biortech.2010.12.077
Ziganshin, A.M., Liebetrau, J., Pröter, J., Kleinsteuber, S.: Microbial community structure and dynamics during anaerobic digestion of various agricultural waste materials. Appl. Microbiol. Biotechnol. 97, 5161–5174 (2013). https://doi.org/10.1007/s00253-013-4867-0
Al Seadi, T., Drosg, B., Fuchs, W., Rutz, D., Janssen, R.: The Biogas Handbook: Science, Production and Applications Biogas Digestate Quality and Utilization. Woodhead Publishing Limited, Cambridge (2013)
Fitamo, T., Boldrin, A., Boe, K., Angelidaki, I., Scheutz, C.: Co-digestion of food and garden waste with mixed sludge from wastewater treatment in continuously stirred tank reactors. Bioresour. Technol. 206, 245–254 (2016). https://doi.org/10.1016/j.biortech.2016.01.085
Buckley, M., Wall, J.D.: Microbial energy conversion (2006). https://www.osti.gov/biblio/895093. Accessed 2 Feb 2018
Wang, X., Lu, X., Li, F., Yang, G.: Effects of temperature and carbon–nitrogen (C/N) ratio on the performance of anaerobic co-digestion of dairy manure, chicken manure and rice straw: focusing on ammonia inhibition. PLoS ONE 9, 1–7 (2014). https://doi.org/10.1371/journal.pone.0097265
Reungsang, A., Pattra, S., Sittijunda, S.: Optimization of key factors affecting methane production from acidic effluent coming from the sugarcane juice hydrogen fermentation process. Energies 5, 4746–4757 (2012). https://doi.org/10.3390/en5114746
Sathish, S., Vivekanandan, S.: Parametric optimization for floating drum anaerobic bio-digester using response surface methodology and artificial neural network. Alex. Eng. J. 55(4), 3297–3307 (2016)
Kainthola, J., Kalamdhad, A.S., Goud, V.V.: Optimization of methane production during anaerobic co-digestion of rice straw and Hydrilla verticillata using response surface methodology. Fuel 235, 92–99 (2019). https://doi.org/10.1016/j.fuel.2018.07.094
Reddy, P.R.M., Ramesh, B., Mrudula, S., Reddy, G., Seenayya, G.: Production of thermostable β-amylase by Clostridium thermosulfurogenes SV2 in solid state fermentation: optimization of nutrient levels using response surface methodology. Process Biochem. 39, 267–277 (2003)
Elibol, M.: Optimization of medium composition for actinorhodin production by Streptomyces coelicolor A3(2) with response surface methodology. Process Biochem. 39, 1057–1062 (2004)
Chen, X., Wang, J.H., Li, D.S.: Optimization of solid-state medium for the production of inulinase by Kluyveromyces S120 using response surface methodology. Biochem. Eng. J. 34, 179–184 (2007)
Yusof, T.R.T., Che-Man, H., Abdul Rahman, N.A., Hafid, H.S.: Optimization of methane gas production from co-digestion of food waste and poultry manure using artificial neural network and response surface methodology. J. Agric. Sci. 6, 27 (2014). https://doi.org/10.5539/jas.v6n7p27
Zhang, Q., Tang, L., Zhang, J., Mao, Z., Jiang, L.: Optimization of thermal-dilute sulfuric acid pretreatment for enhancement of methane production from cassava residues. Bioresour. Technol. 102(4), 3958–3965 (2011)
Sarabia, L.A., Ortiz, M.C.: Response surface methodology. In: Comprehensive Chemometrics, vol. 1, pp. 345–390. Elsevier, Amsterdam (2009)
Ellis, G.V., Schmidt, S.: Anaerobic digestion of zebra, elephant, wildebeest and impala drop**s―assessment of the presence of methanogens and biogas yields. In: International IWA Symposium on Anaerobic Digestion of Solid Waste and Energy Crops (ADSW&EC), 28 August––1 September, Vienna, Austria, p. 5 (2011)
Chong, M.L., Abdul Rahman, N., Yee, P.L., Aziz, S.A., Rahim, R.A., Shirai, Y., Hassan, M.A.: Effects of pH, glucose and iron sulfate concentration on the yield of biohydrogen by Clostridium butyricum EB6. Int. J. Hydrog. Energy 34, 8859–8865 (2009)
American Public Health Association, APHA: Standard Methods for the Examination of Water and Wastewater. American Water Works Association and Water Environment Federation, Washington, DC (2005)
Da Ros, C., Cavinato, C., Cecchi, F., Bolzonella, D.: Anaerobic co-digestion of winery waste and waste activated sludge: assessment of process feasibility. Water Sci. Technol. 69, 269–277 (2014)
Carotenuto, C., Guarino, G., Morrone, B., Minale, M.: Temperature and pH effect on methane production from buffalo manure anaerobic digestion. Int. J. Heat Technol. 34, 623–629 (2016). https://doi.org/10.18280/ijht.34S233
Luo, G., **e, L., Zou, Z., Zhou, Q., Wang, J.Y.: Fermentative hydrogen production from cassava stillage by mixed anaerobic microflora: effects of temperature and pH. Appl. Energy (2010). https://doi.org/10.1016/j.apenergy.2010.07.004
Henze, M.: Basic biological processes. In: Henze, M., Harremoes, P., Cour, Jansen J., Arvin, E. (eds.) Wastewater Treatment: Biological and Chemical Processes, pp. 65–129. Springer, Berlin (2002)
Chanathaworn, J.: Operating condition optimization of water hyacinth and earthworm bedding wastewater for biogas production. Energy Procedia 138, 253–259 (2017)
Van Kessel, J.A.S., Russell, J.B.: The effect of pH on ruminal methanogenesis. FEMS Microbiol. Ecol. 20, 205–210 (1996)
Adelekan, B., Bamgboye, A.: Comparison of biogas productivity of cassava peels mixed in selected ratios with major livestock waste types. Afr. J. Agric. Res. 4, 571–577 (2009)
Ghaly, A.E., Ramkumar, D.R., Sadaka, S.S., Rochon, J.D.: Effect of reseeding and pH control on the performance of a two-stage mesophilic anaerobic digester operating on acid cheese whey. Can. Agric. Eng. 42, 173–183 (2000)
Boontian, N.: Conditions of the anaerobic digestion of biomass. Int. J. Biol. Biomol. Agric. Food Biotechnol. Eng. (2014). https://doi.org/10.5281/zenodo.1096285
Lin, Q., He, G., Rui, J., Fang, X., Tao, Y., Li, J., Li, X.: Microorganism-regulated mechanisms of temperature effects on the performance of anaerobic digestion. Microb. Cell Factories 15(1), 96 (2016)
Tait, S., Astals, S., Batstone, D., Jensen, P.: Enhanced methane bioenergy recovery at Australian piggeries through anaerobic co-digestion 4C-113. Final report prepared for the Cooperative Research Centre for High Integrity Australian Pork, Queensland (2018). https://porkcrc.com.au/wp-content/uploads/2018/02/4C-113-Project-Final-Research-Report.pdf. Accessed 6 Sept 2018
Schnürer, A., Jarvis, A.: Microbiological Handbook for Biogas Plants. Swedish Waste Management, Swedish Gas Centre, Malmö (2010)
Yan, Z., Song, Z., Li, D., Yuan, Y., Liu, X., Zheng, T.: The effects of initial substrate concentration, C/N ratio, and temperature on solid-state anaerobic digestion from composting rice straw. Bioresour. Technol. 177, 266–273 (2015). https://doi.org/10.1016/j.biortech.2014.11.089
Acknowledgements
This work is based on the research supported in part by the National Research Foundation (NRF) of South Africa for the Grant, Thuthuka Unique Grant No. 99393. Our sincere thanks goes the staff of the Agricultural Research Council (ARC) Stellenbosch, the Language Editor Dr. Solani Ngobeni from the Centre for Scholarly Publishing, Mr. Taiwo Abiola for assistance in modelling and the Staff of the Research Directorate at Cape Peninsula University of Technology for their support.
Author information
Authors and Affiliations
Corresponding author
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Mkruqulwa, U., Okudoh, V. & Oyekola, O. Optimizing Methane Production from Co-digestion of Cassava Biomass and Winery Solid Waste Using Response Surface Methodology. Waste Biomass Valor 11, 4799–4808 (2020). https://doi.org/10.1007/s12649-019-00801-y
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12649-019-00801-y