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The Evaluation of Different Bacteriocinogenic Bacillus spp. with Activity Against Staphylococcus spp. and Their Beneficial and/or Hazardous Properties

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Abstract

The aim of this project was to screen for bacteriocinogenic Bacillus strains with activity versus Staphylococcus spp. with future application in formulation of pharmaceutical antimicrobial preparations. Putative bacteriocinogenic strains, isolated and pre-identified as Bacillus spp. were selected for future study and differentiated based on repPCR and identified as Bacillus subtilis for strains ST826CD and ST829CD, Bacillus subtilis subsp. stercoris for strain ST794CD, Bacillus subtilis subsp. spizizenii for strain ST824CD, Bacillus velezensis for strain ST796CD, and Bacillus tequilensis for strain ST790CD. Selected strains were evaluated regarding their safety/virulence, beneficial properties, and potential production of antimicrobials based on biomolecular and physiological approves. Expressed bacteriocins were characterized regarding their proteinaceous nature, stability at different levels of pH, temperatures, and the presence of common chemicals applied in bacterial cultivation and bacteriocin purification. Dynamic of bacterial growth, acidification, and cumulation of produced bacteriocins and some aspects of the bacteriocins mode of action were evaluated. Based on obtained results, isolation and application of expressed antimicrobials can be realistic scenario for treatment of some staphylococcal associated infections. Appropriate biotechnological approaches need to be developed for cost effective production, isolation, and purification of expressed antimicrobials by studied Bacillus strains.

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All data generated by this project is available upon request.

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Funding

This study was performed and supported by Handong Global University, Pohang, Republic of Korea. The authors acknowledge Mia Miau for her unconditional support.

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Authors and Affiliations

  1. ProBacLab, Department of Advanced Convergence, Handong Global University, Pohang, Gyeongbuk, 37554, Republic of Korea

    Joanna Ivy Irorita Fugaban, Clarizza May Dioso, Gee Hyeun Choi, Jorge Enrique Vazquez Bucheli & Svetoslav Dimitrov Todorov

  2. National Food Institute, Technical University of Denmark, Building 202, Rm. 3.234, Kemitorvet, Kongens, Lyngby, 2800, Denmark

    Joanna Ivy Irorita Fugaban

  3. School of Industrial Technology, Universiti Sains Malaysia, Penang, 11800, Malaysia

    Min-Tze Liong

  4. Human Effective Microbes, Department of Advanced Convergence, Handong Global University, Pohang, Gyeongbuk, 37554, Republic of Korea

    Wilhelm Heinrich Holzapfel

  5. ProBacLab, Laboratório de Microbiologia de Alimentos, Departamento de Alimentos e Nutrição Experimental, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, São Paulo (SP), Brazil

    Svetoslav Dimitrov Todorov

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  1. Joanna Ivy Irorita Fugaban
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  2. Clarizza May Dioso
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  6. Wilhelm Heinrich Holzapfel
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  7. Svetoslav Dimitrov Todorov
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Contributions

Concept: SDT; experimental work: SDT, JIIF, CMD, GHC, and JEVB; funds: WHH, SDT; writing of the manuscript: SDT, CMD, and JIIF; corrections and editing: WHH, MTL, and SDT All authors have read and agreed to the published version of the manuscript.

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Correspondence to Svetoslav Dimitrov Todorov.

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Fugaban, J.I.I., Dioso, C.M., Choi, G.H. et al. The Evaluation of Different Bacteriocinogenic Bacillus spp. with Activity Against Staphylococcus spp. and Their Beneficial and/or Hazardous Properties. Probiotics & Antimicro. Prot. 16, 35–52 (2024). https://doi.org/10.1007/s12602-022-10017-7

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