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Identification and pathogenicity of Lasiodiplodia and Neoscytalidium species associated with mango (Mangifera indica) dieback disease in Israel

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Abstract

Mango is affected by several fungal diseases, including dieback caused by species of the Botryosphaeriaceae family. Recently, mango dieback was reported from multiple locations in Israel. In this study, we isolated and characterized 11 representative fungal isolates belonging to Botryosphaeriaceae from symptomatic mango stems. Lasiodiplodia theobromae and Neoscytalidium dimidiatum are reported in this study as pathogens causing mango dieback in Israel, based on morphology, phylogenetic inference (using ITS and tef1-α regions) and pathogenicity assays. Representative isolates of L. theobromae and N. dimidiatum caused dieback symptoms on detached mango stems under controlled conditions and on mango trees outdoors, growing under natural conditions under shade nets. The pathogens were re-isolated from inoculated stems, fulfilling Koch’s postulates. Lasiodiplodia theobromae has not been previously reported in Israel, while N. dimidiatum has been recorded on pitahaya fruit causing internal black rot disease. In this study, we report in detail on mango dieback caused by L. theobromae and N. dimidiatum in Israel. Considering the economic impact of dieback disease on mango yield, further studies on epidemiology will assist in develo** disease management strategies.

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Data availability

The ITS and tef1-α gene sequences generated in this study and used in the phylogenetic analyses were submitted to GenBank with accession numbers presented in Table 1.

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Acknowledgements

The work was partially supported by the US Agency for International Development, under the terms of Middle East Regional Cooperation Program Award No. SIS70022GR0025. GS and VM thank ARO for postdoctoral fellowships. The authors thank Ran Shulhani for his help in the pathogenicity assays.

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  1. Gunjan Sharma

    Present address: Department of Plant Biotechnology, Gujarat Biotechnology University, Gandhinagar, Gujarat, India

  2. Vineet Meshram

    Present address: Department of Biotechnology and Microbiology, Anjaneya University, Raipur, Chhattisgarh, 492101, India

  3. Gunjan Sharma and Meirav Elazar contributed equally to this work.

Authors and Affiliations

  1. Department of Plant Pathology and Weed Research, Institute of Plant Protection, Agricultural Research Organization, Volcani Institute, Rishon LeZion, 7505101, Israel

    Gunjan Sharma, Meirav Elazar, Marcel Maymon, Vineet Meshram & Stanley Freeman

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  1. Gunjan Sharma
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SF conceived the study and arranged the fund. GS, ME, and MM performed the experiments and analyzed the data. GS and SF wrote the manuscript. VM performed the statistical analysis. All authors have read and approved the final version of the manuscript.

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Correspondence to Stanley Freeman.

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Sharma, G., Elazar, M., Maymon, M. et al. Identification and pathogenicity of Lasiodiplodia and Neoscytalidium species associated with mango (Mangifera indica) dieback disease in Israel. Phytoparasitica 52, 8 (2024). https://doi.org/10.1007/s12600-024-01123-z

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