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Heart-Type Fatty Acid-Binding Protein, in Early Detection of Acute Myocardial Infarction: Comparison with CK-MB, Troponin I and Myoglobin

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Abstract

The study aimed to investigate whether heart-type fatty acid binding protein (H-FABP) measurement provides additional diagnostic value to that of conventional cardiac markers in acute myocardial infarction (AMI) within first 6 h after the onset of symptoms. The study included 120 subjects: 60 AMI cases and 60 age and sex matched controls. The cases and controls were further divided into 2 subgroups depending on the time since onset of chest pain as (1) subjects within 3 h and (2) between 3 and 6 h of onset of chest pain. In all the cases and controls, serum H-FABP concentration was measured by Immunoturbidimetric method, serum Troponin I and myoglobin concentrations by Chemiluminescence immunoassay and serum CK-MB concentration by Immuno-inhibition method. The sensitivity, specificity, positive and negative predictive values of H-FABP were significantly greater than CK-MB and myoglobin but were lesser than Troponin I in patients with suspected AMI in both within 3 h and 3–6 h groups. Receiver operating characteristic curves demonstrated greatest diagnostic ability for Troponin I (AUC = 0.99, p < 0.001) followed by H-FABP (AUC = 0.906, p < 0.001) within 3 h and 3–6 h after the onset of chest pain. In conclusion, the diagnostic value of H-FABP is greater than CK-MB and myoglobin but slightly lesser than troponin I for the early diagnosis of AMI within first 6 h of chest pain. H-FABP can be used as an additional diagnostic tool for the early diagnosis of AMI along with troponin I.

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Acknowledgments

We would like to thank BLDE University, Vijayapur, Karnataka for the research grant towards this study. We thank the staff of the Department of Medicine and Department of Biochemistry, BLDEU’s Shri B M Patil Medical College, Hospital and Research Centre, Vijayapur for Their constant support and motivation for the collection of cases and carrying out investigations. Special thanks to Dr Kusal K Das, Professor, Department of Physiology, BLDEU’s Shri B M Patil Medical College, for valuable suggestions and inspiration. We also thank all the study subjects who took part in this study.

Funding

This study was funded by BLDE University, Vijayapur, 586103, Karnataka, India.

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Authors and Affiliations

  1. Department of Biochemistry, BLDE University’s Shri B M Patil Medical College, Hospital and Research Centre, Vijayapur, Karnataka, India

    Anand K. Pyati & Basavaraj B. Devaranavadagi

  2. Department of Cardiology, BLDE University’s Shri B M Patil Medical College, Hospital and Research Centre, Vijayapur, Karnataka, India

    Sanjeev L. Sajjannar

  3. Department of Biochemistry, Belagavi Institute of Medical Sciences (BIMS), Dr B R Ambedkar Road, Belagavi, Karnataka, 590001, India

    Anand K. Pyati & Shashikant V. Nikam

  4. Department of Community Medicine, BLDE University’s Shri B M Patil Medical College, Hospital and Research Centre, Vijayapur, Karnataka, India

    Mohd. Shannawaz

  5. Department of Physiology, BLDE University’s Shri B M Patil Medical College, Hospital and Research Centre, Vijayapur, Karnataka, India

    Satish Patil

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  1. Anand K. Pyati
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  2. Basavaraj B. Devaranavadagi
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Corresponding author

Correspondence to Anand K. Pyati.

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Conflict of interest

Dr Anand K. Pyati, Dr Basavaraj B. Devaranavadagi, Dr Sanjeev L. Sajjannar, Dr Shashikant V. Nikam, Dr Mohd Shannawaz and Dr Satish Patil declare that they have no conflict of interest.

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All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

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Pyati, A.K., Devaranavadagi, B.B., Sajjannar, S.L. et al. Heart-Type Fatty Acid-Binding Protein, in Early Detection of Acute Myocardial Infarction: Comparison with CK-MB, Troponin I and Myoglobin. Ind J Clin Biochem 31, 439–445 (2016). https://doi.org/10.1007/s12291-015-0544-7

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  • DOI: https://doi.org/10.1007/s12291-015-0544-7

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