SpringerLink
Log in

A Cross Sectional Study on T Cells and Subsets in Chronic Lymphocytic Leukemia and their Association with Clinical Phenotype

  • Original Article
  • Published:
Indian Journal of Hematology and Blood Transfusion Aims and scope Submit manuscript

Abstract

T cells in B chronic lymphocytic leukaemia (CLL) have been reported to show qualitative and quantitative alterations, thereby regulating the antitumor immune response. We evaluated the absolute count, percentages of T cells and subsets and their association with disease parameters in Indian patients. 45 treatment naïve CLL cases and 10 healthy controls were evaluated for CD3 + Tcells, CD4 + T cells, CD8 + T cells percentage by flowcytometry. The absolute counts were obtained by multiplication with absolute lymphocyte counts obtained on cell counter. The clinical characteristics (age, sex, Rai stage, B symptoms, CD38 expression) were analysed for any association with alteration of these cells (percentages, absolute counts, ratio with monoclonal B cell count) and CD4: CD8 ratio. The mean absolute count of CD3 + T cell, CD4 + T cell and CD8 + T cells was significantly higher (p < 0.05) in CLL patients as compared to healthy controls. CD4:CD8 ratio was variable (normal, increased and decreased). The mean CD8 + T cells count was higher in advanced disease stage, and CD38 positive cases (p > 0.05). Younger CLL patients (< 55 years) had greater increase in CD8 + T cells (p > 0.05). Significant alterations in T cells and their subsets were observed in CLL. A trend towards advanced stage, CD38 expression, presentation an early age was seen with increase in CD8 + T cell counts.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price includes VAT (France)

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Jain M, Kushwaha R, Verma SP, Singh US, Tripathi AK, Goel MM, Kumar A (2017) CD8 expression in b chronic lymphocytic leukemia: a case of immuno‐phenotypic aberrancy. Cytom B Clin Cytom 94(4):679–682

  2. Krackhardt AM, Harig S, Witzens M, Broderick R, Barrett P, Gribben JG (2002) T-cell responses against chronic lymphocytic leukemia cells: implications for immunotherapy. Blood 100(1):167–173

    Article  CAS  Google Scholar 

  3. Correia RP, Bacal NS, Campregher PV, Hamerschlak N, Amarante-Mendes GP (2014) Involvement of memory T-cells in the pathophysiology of chronic lymphocytic leukemia. Revista Brasileira de Hematologia e Hemoterapia 36(1):60–64

    Article  Google Scholar 

  4. Porakishvili N, Roschupkina T, Kalber T, Jewell AP, Patterson K, Yong K, Lydyard PM (2001) Expansion of CD4+ T cells with a cytotoxic phenotype in patients with B-chronic lymphocytic leukaemia (B-CLL). Clin Exp Immunol 126(1):29–36

    Article  CAS  Google Scholar 

  5. Lad DP, Varma S, Varma N, Sachdeva MU, Bose P, Malhotra P (2013) Regulatory T-cells in B-cell chronic lymphocytic leukemia: their role in disease progression and autoimmune cytopenias. Leukemia Loymphoma 54(5):1012–1019

    Article  CAS  Google Scholar 

  6. Wu J, Xu X, Lee EJ, Shull AY, Pei L, Awan F, Wang X, Choi JH, Deng L, **n HB, Zhong W (2016) Phenotypic alteration of CD8+ T cells in chronic lymphocytic leukemia is associated with epigenetic reprogramming. Oncotarget 7(26):40558

    Article  Google Scholar 

  7. Alaaldeen SA, AM RO, AA DA, AA AM, Ali O (2016) Prognostic significance of immune function parameters (CD4, CD8 and CD4/CD8 ratio) in Sudanese patients with chronic lymphocytic leukemia. Int J Multidiscip Curr Res 4:650–653

  8. Gonzalez-Rodriguez AP, Contesti J, Huergo-Zapico L, Lopez-Soto A, Fernandez-Guizan A, Acebes-Huerta A, Gonzalez-Huerta AJ, Gonzalez E, Fernandez-Alvarez C, Gonzalez S (2010) Prognostic significance of CD8 and CD4 T cells in chronic lymphocytic leukemia. Leuk Lymph 51(10):1829–1836

    Article  CAS  Google Scholar 

  9. Yang SM, Li JY, Gale RP, Huang XJ (2015) The mystery of chronic lymphocytic leukemia (CLL): Why is it absent in Asians and what does this tell us about etiology, pathogenesis and biology? Blood Rev 29(3):205–213

    Article  Google Scholar 

  10. Matrai Z (2005) CD38 as a prognostic marker in CLL. Hematology 10(1):39–46

    Article  CAS  Google Scholar 

  11. Calvelli T, Denny TN, Paxton H, Gelman R, Kagan J (1993) Guideline for flow cytometric immunophenoty**: a report from the national institute of allergy and infectious diseases, division of AIDS. Cytometry 14(7):702–715

    Article  CAS  Google Scholar 

  12. Saxena RK, Choudhry V, Nath I (2004) Normal ranges of some select lymphocyte subpopulations in peripheral blood of normal healthy Indians. Curr Sci 86:969–975

    Google Scholar 

  13. Parikh SA, Rabe KG, Kay NE, Call TG, Ding W, Schwager S, Jelinek DF, Slager SL, Shanafelt TD (2014) Chronic lymphocytic leukemia in young (<55 years) patients: a comprehensive analysis of prognostic factors and outcomes. Haematologica 99(1):140–147

Download references

Author information

Authors and Affiliations

  1. Department of Pathology, King George’s Medical University, Lucknow, 226003, India

    Mili Jain, Anushree Rai, Rashmi Kushwaha, Geeta Yadav, Sanjay Mishra, Uma Shankar Singh & Ashutosh Kumar

  2. Department of Clinical Hematology, King George’s Medical University, Lucknow, India

    Shailendra Prasad Verma & Anil Kumar Tripathi

Authors
  1. Mili Jain
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Anushree Rai
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Rashmi Kushwaha
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Geeta Yadav
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Sanjay Mishra
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Shailendra Prasad Verma
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Uma Shankar Singh
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Anil Kumar Tripathi
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Ashutosh Kumar
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Mili Jain.

Ethics declarations

Conflict of interest

The author declared that there is no conflict of interest.

Ethical Approval

Approved by the institutional ethics committee.

Consent to Participate

Informed consent taken.

Consent for Publication

Informed consent taken.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Jain, M., Rai, A., Kushwaha, R. et al. A Cross Sectional Study on T Cells and Subsets in Chronic Lymphocytic Leukemia and their Association with Clinical Phenotype. Indian J Hematol Blood Transfus 37, 563–568 (2021). https://doi.org/10.1007/s12288-021-01397-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12288-021-01397-x

Keywords

Search

Navigation