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Spirosoma luteolum sp. nov. isolated from water

  • Microbial Systematics and Evolutionary Microbiology
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Abstract

A novel Gram-negative and rod-shaped bacterial strain, designated as 16F6ET, was isolated from a water sample. Cells were yellowish in color and catalase- and oxidase-positive. The strain grew at 10–37°C (optimum at 25°C) but not at 4 and 42°C, and pH 5–7 (optimum at pH 7). It showed moderate resistance to gamma-ray irradiation. Comparative phylogenetic analysis showed that strain 16F6ET belonged to the family Cytophagaceae of the class Cytophagia. Furthermore, this isolate showed relatively low 16S rRNA gene sequence similarities (90.7–93.1%) to the members of the genus Spirosoma. The major fatty acids were summed feature 3 (C16:1 ω7c/C16:1 ω6c), C16:1 ω5c, C16:0 N alcohol, and C16:0. The polar lipid profile indicated presence of phosphatidylethanolamine, unknown aminophospholipids, an unknown amino lipid, unknown phospholipids, and unknown polar lipids. The predominant isoprenoid quinone was MK-7. The genomic DNA G+C content of strain 16F6ET was 56.5 mol%. Phenotypic, phylogenetic, and chemotaxonomic properties indicated that isolate 16F6ET represents a novel species within the genus Spirosoma, for which the name Spirosoma luteolum sp. nov. is proposed. The type strain is 16F6ET (=KCTC 52199T =JCM 31411T).

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Authors and Affiliations

  1. School of Applied Biosciences, Kyungpook National University, Daegu, 41566, Republic of Korea

    Jae-** Lee, Su-** Park, Yeon-Hee Lee, Seung-Yeol Lee, Sangkyu Park, Leonid N. Ten & Hee-Young Jung

  2. School of Food Science and Biotechnology/Food and Bio-Industry Research Institute, Kyungpook National University, Daegu, 41566, Republic of Korea

    Young-Je Cho

  3. Department of Bio and Environmental Technology, Seoul Women’s University, Seoul, 01797, Republic of Korea

    Myung Kyum Kim

  4. Institute of Plant Medicine, Kyungpook National University, Daegu, 41566, Republic of Korea

    Hee-Young Jung

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  1. Jae-** Lee
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Correspondence to Hee-Young Jung.

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Supplemental material for this article may be found at http://www.springerlink.com/content/120956.

The NCBI GenBank/EMBL/DDBJ accession number for the 16S rRNA g ene sequence of strain 16F6ET is KU758884.

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Lee, JJ., Park, SJ., Lee, YH. et al. Spirosoma luteolum sp. nov. isolated from water. J Microbiol. 55, 247–252 (2017). https://doi.org/10.1007/s12275-017-6455-y

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  • DOI: https://doi.org/10.1007/s12275-017-6455-y

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