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Biophysical-driven piezoelectric and aligned nanofibrous scaffold promotes bone regeneration by re-establishing physiological electrical microenvironment

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Abstract

The initial healing stages of bone fracture is a complex physiological process involving a series of spatially and temporally overlap** events, including pathogen clearance, immunological modulation, and osteogenesis. In this study, we have developed a piezoelectric and aligned nanofibrous scaffold composed of ZnO@PCL/PVDF with multiple antibacterial, immunomodulatory, and osteogenic effects using electrospinning technology. This scaffold’s piezoelectric signal output under ultrasound (US) control can be similar to the physiological electrical signals of healthy bone tissue, creating a truly biomimetic electrical microenvironment in the bone defect. In vitro studies have shown that ZnO@PCL/PVDF scaffold significantly enhances the proliferation, migration, and osteogenic differentiation of MC3T3-E1 cells under piezoelectric drive provided by ultrasound. Furthermore, the scaffold exhibits inhibitory effects on the growth of E. coli and S. aureus, as well as the ability to induce M2 macrophage polarization, indicating potent antibacterial and immunomodulatory properties. In vivo experiments demonstrated that the ZnO@PCL/PVDF scaffold can accelerate the repair of mandibular defects in rats, effectively inhibit bacterial colonization, and reduce inflammatory responses. Altogether, this study confirms that the newly developed ZnO@PCL/PVDF scaffold effectively promotes bone repair by truly mimicking the endogenous electrical microenvironment and precisely regulating the temporospatial disorders of initial bone healing, thus providing a simple and effective solution for bone defects.

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Acknowledgements

This work was funded by National Natural Science Foundation of China (Nos. 82151312, 82272493, and 82072406). The Bei**g Science Nova Program (No. 20220484155). The Natural Science Foundation of Shaanxi Province (No. 2023-YBSF-426). Bei**g Jishuitan Hospital Elite Young Scholar Programme (No. XKGG2021).

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Author notes

  1. Aoao Wang, **nbo Ma, and Yafeng Yang contributed equally to this work.

Authors and Affiliations

  1. Senior Department of Orthopedics, the Fourth Medical Center of PLA General Hospital, No. 51, Fucheng Road, Haidian District, Bei**g, 100048, China

    Aoao Wang, Yafeng Yang, Guoliang Shi, Liwei Han, **antong Hu & Yantao Zhao

  2. Department of Chemistry, Capital Normal University, No. 105 West 3S1 Ring North Rd, Bei**g, 100048, China

    **nbo Ma

  3. National Center for Orthopaedics; Bei**g Research Institute of Traumatology and Orthopaedics; Bei**g Jishuitan Hospital, Capital Medical University, **cheng District **njiekou No. 31 East Street, Bei**g, 100035, China

    Rui Shi

  4. ****g 986 Hospital Department, The Fourth Military Medical University, **’an, 710032, China

    Jun Yan

  5. Institute of Orthopedics, Chinese PLA General Hospital; Bei**g Key Laboratory of Regenerative Medicine in Orthopedics; Key Laboratory of Musculoskeletal Trauma & War Injuries PLA, No. 28 Fuxing Road, Haidian District, Bei**g, 100853, China

    Quanyi Guo

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  1. Aoao Wang
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Correspondence to Rui Shi, Jun Yan, Quanyi Guo or Yantao Zhao.

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Biophysical-driven piezoelectric and aligned nanofibrous scaffold promotes bone regeneration by re-establishing physiological electrical microenvironment

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Wang, A., Ma, X., Yang, Y. et al. Biophysical-driven piezoelectric and aligned nanofibrous scaffold promotes bone regeneration by re-establishing physiological electrical microenvironment. Nano Res. (2024). https://doi.org/10.1007/s12274-024-6673-7

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