SpringerLink
Log in

Development of Molecular Markers Closely Linked to the Potato Leafroll Virus Resistance Gene, Rlr etb , for use in Marker-Assisted Selection

  • Published:
American Journal of Potato Research Aims and scope Submit manuscript

Abstract

Potato leafroll virus (PLRV) is a major pathogen of potato with worldwide impact on seed and commercial production of potato. In North America, the primary potato cultivars grown by industry are not resistant to PLRV and require the application of insecticides to control the aphid vector of PLRV, so as to prevent PLRV infection of the crop. The Solanum etuberosum- derived dominant gene Rlr etb confers resistance to PLRV and has been mapped to chromosome 4. The previous closest marker to Rlr etb , C2_At1g42990, was mapped 13.6 cM from the gene in a BC3 population. Cleaved amplified polymorphic sequence markers were developed in the region surrounding C2_At1g42990 and mapped along with Rlr etb in 102 BC4 progeny. Sixteen markers were identified surrounding Rlr etb , with flanking markers at 2.1 and 9.3 cM. Two markers, 1367-8a and DMB32-11, both 2.1 cM from Rlr etb , are shown to be well suited for marker assisted selection. The development of additional molecular markers more closely linked to Rlr etb will greatly facilitate its use in potato breeding and its continued introgression into cultivated potato for the development of PLRV-resistant cultivars.

Resumen

El virus del enrollamiento de la hoja de la papa (PLRV) es un patógeno importante de la papa con impacto mundial en la producción de semilla y en la producción comercial de la papa. En Norteamérica, las principales variedades de papa que se cultivan por la industria no son resistentes al PLRV y requieren de la aplicación de insecticidas para control del áfido vector del PLRV, así como para prevenir la infección del cultivo por PLRV. El gen dominante derivado de Solanum etuberosum, Rlretb, confiere resistencia al PLRV y ha sido ubicado en el cromosoma 4. El marcador previo más cercano al Rlretb, C2_At1g42990, fue ubicado a 13.6 cM del gen en una población BC3. Se desarrollaron marcadores fragmentados amplificados de secuencias polimórficas en la región que rodea a C2_At1g42990 y se les hizo un mapa junto con Rlretb en una progenie 102 BC4. Se identificaron 16 marcadores rodeando al Rlretb, con marcadores en los lados a 2.1 y 9.3 cM. Dos marcadores, 1367-8a y DMB32-11, ambos a 2.1 cM de Rlretb, se ha demostrado que son los apropiados para la selección asistida por marcadores. El desarrollo de marcadores moleculares adicionales más cercanamente ligados a Rlretb facilitará grandemente su uso en el mejoramiento de la papa así como su incorporación a papa cultivada para el desarrollo de variedades resistentes al PLRV.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  • Ahmadvand R., P. Poczai, R. Hajianfar, B. Kolics, A.M. Gorji, Z. Polgár, and J. Taller. 2014. Next generation sequencing based development of intron-targeting markers in tetraploid potato and their transferability to other Solanum species. Gene 540: 117–121.

    Article  CAS  PubMed  Google Scholar 

  • Ahn Y.K., J.W. Bang, G.S. Choi, and H.G. Park. 2001. Introgression of resistance to PLRV and PVY from solanum brevidens into S. tuberosum (‘superior’, ‘Dejima’, and dihaploid of ‘superior’) by interspecific somatic hybrids. Journal of the Korean Society for Horticultural Science 42: 707–711.

    Google Scholar 

  • Alseekh S., I. Ofner, T. Pleban, P. Tripodi, F.D. Dato, M. Cammareri, A. Mohammad, S. Grandillo, A.R. Fernie, and D. Zamir. 2013. Resolution by recombination: breaking up Solanum pennellii introgressions. Trends in Plant Science 18: 536–538.

    Article  CAS  PubMed  Google Scholar 

  • Brown C.R., and P.E. Thomas. 1994. Resistance to potato leafroll virus derived from Solanum chacoense: characterization and inheritance. Euphytica 74: 51–57.

    Article  Google Scholar 

  • Carrasco A., J.I. Ruiz de Galarreta, A. Rico, and E. Ritter. 2000. Transfer of PLRV resistance from Solanum verrucosum schlechdt to potato (S. tuberosum L.) by protoplast electrofusion. Potato Research 43: 31–42.

    Article  CAS  Google Scholar 

  • Chanter D.O. 1975. Modifications of the angular transformation. J. R statistic Soc. Series C (Applied Statistics) 24: 354–359.

    Google Scholar 

  • Corsini D.L., J.J. Pavek, M.W. Martin, and C.R. Brown. 1994. Potato germplasm with combined resistance to leafroll virus and viruses X and Y. American Potato Journal 71: 377–385.

    Article  Google Scholar 

  • Felcher K.J., J.J. Coombs, A.N. Massa, C.N. Hansey, J.P. Hamilton, R.E. Veilleux, C.R. Buell, and D.S. Douches. 2012. Integration of two diploid potato linkage maps with the potato genome sequence. PloS One 7: e36347.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Flis B., and I. Wasilewicz-Flis. 1998. Progeny tests to identify diploid potato clones homozygous at loci controlling resistance to PLRV. Potato Research 41: 219–228.

    Article  Google Scholar 

  • Gavrilenko T., R. Thieme, U. Heimback, and T. Thieme. 2003. Fertile somatic hybrids of Solanum etuberosum (+) dihaploid Solanum tuberosum and their backcrossing progenies: relationships of genome dosage with tuber development and resistance to potato virus Y. Euphytica 131: 323–332.

    Article  CAS  Google Scholar 

  • Gillen A.M., and R.G. Novy. 2007. Molecular characterization of the progeny of Solanum etuberosum identifies a genomic region associated with resistance to potato leafroll virus. Euphytica 155: 403–415.

    Article  CAS  Google Scholar 

  • Hirsch C.D., J.P. Hamilton, K.L. Childs, J. Cepela, E. Crisovan, B. Vaillancourt, C.N. Hirsch, M. Habermann, B. Neal, and C.R. Buell. 2014. Spud DB: a resource for mining sequences, genotypes, and phenotypes to accelerate potato breeding. Plant Genome 7: 1–12.

    Article  Google Scholar 

  • Jansky, S.H. 2000. Breeding for disease resistance in potato. In: Plant breeding reviews, ed. J. Janick, 69–155. New York: Wiley.

  • Kelley K.B., J.L. Whitworth, and R.G. Novy. 2009. Map** of the potato leafroll and virus resistance gene, Rlr etb , from Solanum etuberosum identifies interchromosomeal translocations among its E-genome chromsomes 4 and 9 relative to the a-genome of solanum L. sect. Petota. Molecular Breeding 23: 489–500.

    Article  CAS  Google Scholar 

  • Manly M.F. 1993. A Macintosh program for storage and analysis of experimental genetic map** data. Mammalian Genome 4: 303–313.

    Article  CAS  PubMed  Google Scholar 

  • Manly K.R., and R.H. Cudmore. 1998. Map manager XP. San Diego: In Abstracts of Plant and Animal Genome VI.

    Google Scholar 

  • Marczewski W., B. Flis, J. Syller, R. Schäfer-Pregl, and C. Gebhardt. 2001. A major quantitative trait locus for resistance to potato leafroll virus is located in a resistance hotspot on potato chromosome XI and is tightly linked to N-gene-like markers. Molecular Plant-Microbe Interactions 14: 1420–1425.

    Article  CAS  PubMed  Google Scholar 

  • Marczewski W., B. Flis, J. Syller, D. Strzelczyk-Żyta, J. Hennig, and C. Gebhardt. 2004. Two allelic or tightly linked genetic factors at the PLRV.4 locus on potato chromosome XI control resistance to potato leafroll virus accumulation. Theoretical Applied Genetics 109: 1604–1609.

    Article  CAS  PubMed  Google Scholar 

  • Matsubayashi, M. 1991. Phylogenetic relationships in the potato and its related species. In: Chromosome engineering in plants: genetics, breeding, evolution part B, ed. T. Tsuchiya and P.K. Gupta. 93–118. Netherlands: Elsevier.

  • McGrath J.M., S.M. Wielgus, T.F. Uchytil, H. Kim-Lee, G.T. Haberlach, C.E. Williams, and J.P. Helgeson. 1994. Recombination of Solanum brevidens chromosomes in the second backcross generation from a somatic hybrid with S. tuberosum. Theoretical and Applied Genetics 88: 917–924.

    Article  CAS  PubMed  Google Scholar 

  • Mihovilovich E., L. Alarcón, A.L. Pérez, J. Alvarado, C. Arellano, and M. Bonierbale. 2007. High levels of heritable resistance to potato leafroll virus (PLRV) in Solanum tuberosum subsp. andigena. Crop Science 47: 1091–1103.

    Article  Google Scholar 

  • Mihovilovich E., M. Aponte, H. Lindqvist-Kreuze, and M. Bonierbale. 2014. An RGA-derived SCAR marker linked to PLRV resistance from Solanum tuberosum ssp. andigena. Plant Molecular Biology Reporter 32: 117–128.

    Article  CAS  Google Scholar 

  • Mowry T.M. 2005. Insecticidal reduction of Potato leafroll virus transmission by Myzus persicae. Annals of Applied Biology 146: 81–88.

    Article  CAS  Google Scholar 

  • Novy R.G., and J.P. Helgeson. 1994. Somatic hybrids between Solanum etuberosum and diploid, tuber bearing Solanum clones. Theoretical and Applied Genetics 89: 775–782.

    CAS  PubMed  Google Scholar 

  • Novy R.G., A. Nasruddin, D.W. Ragsdale, and E.B. Radcliffe. 2002. Genetic resistances to potato leafroll virus, potato virus Y, and green peach aphid in progeny of Solanum etuberosum. American Journal of Potato Research 79: 9–18.

    Article  Google Scholar 

  • Novy R.G., A.M. Gillen, and J.L. Whitworth. 2007. Characterization of the expression and inheritance of potato leafroll virus (PLRV) and potato virus Y (PVY) resistance in three generations of germplasm derived from Solanum etuberosum. Theoretical and Applied Genetics 114: 1161–1172.

    Article  CAS  PubMed  Google Scholar 

  • Parokonny A.S., J.A. Marshall, M.D. Bennett, E.C. Cocking, M.R. Davey, and J.B. Power. 1997. Homoeologous pairing and recombination in backcross derivatives of tomato somatic hybrids [Lycopersicon esculentum (+) L. peruvianum]. Theoretical and Applied Genetics 94: 713–723.

    Article  CAS  Google Scholar 

  • Perez F., A. Menendez, P. Dehal, and C.F. Quiros. 1999. Genomic structural differentiation in solanum: comparative map** of the a- and E-genomes. Theoretical and Applied Genetics 98: 1183–1193.

    Article  CAS  Google Scholar 

  • PGSC. 2011. Genome sequence and analysis of the tuber crop potato. Nature 475: 189–197.

    Article  Google Scholar 

  • Poczai P., I. Cernák, A.M. Gorji, S. Nagy, J. Taller, and Z. Polgár. 2010. Development of intron targeting (IT) markers for potato and cross-species amplification in Solanum nigrum (Solanaceae). American Journal of Botany 97: e142–e145.

    Article  CAS  PubMed  Google Scholar 

  • Ramanna M.S., and J.G.T.H. Hermsen. 1981. Structural hybridity in the series etuberosa of the genus solanum and its bearing on crossability. Euphytica 30: 15–31.

    Article  Google Scholar 

  • Solomon-Blackburn R.M., and H. Barker. 2001. Breeding virus resistant potatoes (Solanum tuberosum): a review of traditional and molecular approaches. Heredity 86: 17–35.

    Article  CAS  PubMed  Google Scholar 

  • Spooner D.M., and R. Hijmans. 2001. Potato systematics and germplasm collecting, 1989–2000. American Journal of Potato Research 78: 237–268.

    Article  Google Scholar 

  • Swiezynski K.M., M.A. Dziewonska, and K. Ostrowska. 1989. Resistance to the potato leafroll virus (PLRV) in diploid potatoes. Plant Breeding 103: 221–227.

    Article  Google Scholar 

  • Syller J. 1996. Potato leafroll virus (PLRV): its transmission and control. Integrated Pest Management Reviews 1: 217–227.

    Article  Google Scholar 

  • Taliansky M., M.A. Mayo, and H. Barker. 2003. Potato leafroll virus: a classic pathogen shows some new tricks. Molecular Plant Pathology 4: 81–89.

    Article  CAS  PubMed  Google Scholar 

  • Tiwari J.K., D. Poonam, S.K. Sarkar, J. Gopal Pandey, and S.R. Kumar. 2010. Molecular and morphological characterization of somatic hybrids between Solanum tuberosum L. and S. etuberosum lindl. Plant cell. Tissue and Organ Culture 103: 175–187.

    Article  CAS  Google Scholar 

  • USDA, ARS, National Genetic Resources Program 2014. Germplasm Resources Information Network - (GRIN). [Online Database] National Germplasm Resources Laboratory, Beltsville, Maryland. Available: http://www.ars-grin.gov/cgi-bin/npgs/html/obs.pl?1189587.

  • Valkonen J.P.T., G. Brigneti, and E. Pehu. 1992a. Resistance to Myzus persicae (Sulz.) in wild potatoes of the series Etuberosa. Acta Agriculturu Scandinavica. Section B. Soil and Plant Science 42: 118–127.

    Google Scholar 

  • Valkonen J.P.T., G. Brigneti, L.F. Salazar, E. Pehu, and R.W. Gibson. 1992b. Interactions of the solanum spp. of the etuberosa group and nine potato-infecting viruses and a viroid. Annals of Applied Biology 120: 301–313.

    Article  Google Scholar 

  • Velásquez A.C., E. Mihovilovich, and M. Bonierbale. 2007. Genetic characterization and map** of major gene resistance to potato leafroll virus in Solanum tuberosum ssp andigena. Theoretical Applied Genetics 114: 1051–1058.

    Article  PubMed  Google Scholar 

Download references

Acknowledgments

The authors would like to thank Dr. Craig Johnson, Brigham Young University-Idaho, Rexburg, Idaho for his statistical expertise and the advice and insights he provided on data transformation and subsequent statistical analyses. This project was supported by the Agriculture and Food Research Initiative competitive grant no. 2012-67013-19414 of the USDA National Institute of Food and Agriculture. Additional support was provided by the University of Idaho, College of Agriculture and Life Sciences.

Author information

Authors and Affiliations

  1. Department of Plant, Soil, and Entomological Sciences, University of Idaho, Moscow, ID, 83844, USA

    Joseph C. Kuhl & Margaret S. Dibble

  2. USDA/ARS, Small Grains and Potato Germplasm Research Unit, 1693 S. 2700 W., Aberdeen, ID, 83210, USA

    Richard G. Novy, Jonathan L. Whitworth, Brian Schneider & Darren Hall

Authors
  1. Joseph C. Kuhl
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Richard G. Novy
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jonathan L. Whitworth
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Margaret S. Dibble
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Brian Schneider
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Darren Hall
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Joseph C. Kuhl.

Ethics declarations

Conflict of Interest

The authors declare that they have no conflict of interest.

Electronic Supplementary material

Supplemental Figure 1

(DOCX 230 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Kuhl, J.C., Novy, R.G., Whitworth, J.L. et al. Development of Molecular Markers Closely Linked to the Potato Leafroll Virus Resistance Gene, Rlr etb , for use in Marker-Assisted Selection. Am. J. Potato Res. 93, 203–212 (2016). https://doi.org/10.1007/s12230-016-9496-6

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12230-016-9496-6

Keywords

Search

Navigation