Abstract
Rapid, accurate, sensitive, and real-time detection of streptomycin (STR) residues in dairy products is very important. In this work, for the first time, the gold nanoflower/polyethyleneimine-functionalized metal organic framework (AuNF-PEI-MIL-101(Cr)) nanocomposites were synthesized via hydrothermal method and characterized using various techniques (SEM, TEM, XRD, FT-IR, and XPS). Then, a novel electrochemical aptasensor was constructed for the detection of STR based on AuNF-PEI-MIL-101(Cr) nanocomposites. The gold electrode (GE) modified with AuNF-PEI-MIL-101(Cr) can immobilize a large number of thiolated STR aptamer via the Au–S bond. When the target STR was in the presence, the current signal was reduced due to the specific binding of STR towards the aptamer. The results indicated that the aptasensor had a wide linear range towards STR (0.01–400 nM) with a low detection limit of 0.003 nM under the optimal conditions. Moreover, the aptasensor exhibited good selectivity, reproducibility, and stability. In addition, the STR in the cow, sheep, and goat milk samples was detected by the spiked recovery method, and satisfactory results were obtained. The method provides an efficient pathway for the sensitive detection of STR.
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The authors declare that the data supporting the findings of this study are available within the article.
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Funding
This study was supported by the Science and Technology Program of **’an City (20NYYF0018) and the Supported by the Fundamental Research Funds for the Central Universities (2021CBLY007).
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All authors contributed to the study conception and design. Yuanyuan Hui wrote the main manuscript. Material preparation, data collection, and analysis were performed by Yuanyuan Hui, Haishuai Peng, Ding Yang, and Weizhe Wang. Linqiang Li, Aiqing Zhao, and Bini Wang revised it critically for important intellectual content. Bini Wang provided financial support. All authors read and approved the final manuscript.
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Yuanyuan Hui declares that he has no conflict. Haishuai Peng declares that he has no conflict of interest. Linqiang Li declares that he has no conflict of interest. Aiqing Zhao declares that he has no conflict of interest. Ding Yang declares that he has no conflict of interest. Weizhe Wang declares that he has no conflict of interest. Bini Wang declares that he has no conflict of interest.
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Highlights
1. The presence of AuNF-PEI-MIL-101(Cr) on the sensor surface increases the aptamer loading efficiency.
2. The developed aptasensor exhibits wide linear range and low detection limit for streptomycin.
3. The aptasensor shows high sensitivity and selectivity.
4. Fabricated aptasensor can be proposed for monitoring other antibiotics in foodstuffs.
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Hui, Y., Peng, H., Li, L. et al. A Sensitive and Selective Electrochemical Aptasensor Based On Gold Nanoflower/Polyethyleneimine (Pei)-Functionalized Metal Organic Framework Nanocomposites for Label-Free Determination of Streptomycin in Milk Samples. Food Anal. Methods 16, 677–688 (2023). https://doi.org/10.1007/s12161-023-02446-z
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DOI: https://doi.org/10.1007/s12161-023-02446-z