SpringerLink
Log in

The Nasal Bacteria Microbiome Comparison Among Fungal Ball Sinusitis, Chronic Sinusitis with Polyps

  • ORIGINAL RESEARCH ARTICLE
  • Published:
Indian Journal of Microbiology Aims and scope Submit manuscript

Abstract

To evaluate the composition of the microbial community of the middle nasal in paranasal sinus fungus ball (FB), chronic sinusitis with nasal polyps (CRSwNP) and healthy controls, providing new insights into the pathogenesis of FB and CRSwNP. Through 16 s rRNA gene high-throughput sequencing to determine the microbial characterization from patients with FB (n = 29) and CRSwNP (n = 10), and healthy controls (n = 4). The FB group had significantly lower αdiversity and significantly different β diversity compared to the other groups. All three groups mainly consisted of four bacterial phyla (Firmicutes, Proteobacteria, Bacteroidetes, Actinobacteria). In the FB group, the highest relative abundance was found in Proteobacteria (47.04%). However, pairwise comparisons resulted in statistically significant differences only for Firmicutes (CRSwNP, p = 0.003, Control, p = 0.008). The CRSwNP group was statistically different from the control group in TM7(p = 0.010), Chloroflexi(p = 0.018) and Bacteroidete(p = 0.027). At the genus level, the FB group had the highest relative abundance of Haemophilus (11.53%), followed by Neisseria (7.39%), and Neisseria abundance (p < 0.001) was significantly different from the remaining two groups. Ruminococcacea abundance (p < 0.001) and Comamonadaceae abundance (p < 0.001) were increased in the CRSwNP group. The relative abundance of Lactobacillus (p < 0.001), Bacteroides S24_7 (p < 0.001), and Desulfovibrio (p < 0.001) was significantly decreased in the FB and CRSwNP groups compared to the control group. The imbalance of the microbial community is related to the pathogenesis of sinusitis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price includes VAT (Germany)

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. Ferreiro JA, Carlson BA, Cody DT 3rd (1997) Paranasal sinus fungus balls. Head Neck 19:481–486. https://doi.org/10.1002/(sici)1097-0347(199709)19:6%3c481::aid-hed4%3e3.0.co;2-v

    Article  CAS  PubMed  Google Scholar 

  2. Durrani Z, Naeem M, Khan MA (2018) Assessment of bacterial infection in patients operated for complications of chronic fungal rhinosinusitis. Pak J Med Health Scis 12:153–156

    Google Scholar 

  3. Lou H, Meng Y, Piao Y et al (2016) Cellular phenoty** of chronic rhinosinusitis with nasal polyps. Rhinology 54:150–159. https://doi.org/10.4193/Rhino15.271

    Article  PubMed  Google Scholar 

  4. Guarner F, Malagelada JR (2003) Gut flora in health and disease. Lancet 361:512–519. https://doi.org/10.1016/S0140-6736(03)12489-0

    Article  PubMed  Google Scholar 

  5. Maynard CL, Elson CO, Hatton RD, Weaver CT (2012) Reciprocal interactions of the intestinal microbiota and immune system. Nature 489:231–241. https://doi.org/10.1038/nature11551

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Turnbaugh PJ, Ley RE, Hamady M, Fraser-Liggett CM, Knight R, Gordon JI (2007) The human microbiome project. Nature 449:804–810. https://doi.org/10.1038/nature06244

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Kim DW, Kim YM, Min JY et al (2020) Clinicopathologic characteristics of paranasal sinus fungus ball: retrospective, multicenter study in Korea. Eur Arch Otorhinolaryngol 277:761–765. https://doi.org/10.1002/lary.27726

    Article  PubMed  Google Scholar 

  8. Agostinis C, Mangogna A, Bossi F, Ricci G, Kishore U, Bulla R (2019) Uterine Immunity and Microbiota: A Shifting Paradigm. Front Immunol 17:2387. https://doi.org/10.3389/fimmu.2019.02387

    Article  CAS  Google Scholar 

  9. He XS, Shi WY (2009) Oral microbiology: past, present and future. Int J Oral Sci 1:47–58. https://doi.org/10.4248/ijos.09029

    Article  PubMed  PubMed Central  Google Scholar 

  10. Gan W, Zhang H, Yang F et al (2021) The influence of nasal bacterial microbiome diversity on the pathogenesis and prognosis of chronic rhinosinusitis patients with polyps. Eur Arch Otorhinolaryngol 278:1075–1088. https://doi.org/10.1007/s00405-020-06370-4

    Article  PubMed  Google Scholar 

  11. Rom D, Bassiouni A, Eykman E et al (2019) The association between disease severity and microbiome in chronic Rhinosinusitis. Laryngoscope 129:1265–1273

    Article  PubMed  Google Scholar 

  12. Wagner Mackenzie B, Waite DW, Hoggard M et al (2017) Bacterial community collapse: a meta-analysis of the sinonasal microbiota in chronic rhinosinusitis. Environ Microbiol 19:381–392. https://doi.org/10.1111/1462-2920.13632

    Article  CAS  PubMed  Google Scholar 

  13. Stammberger H, Jakse R, Beaufort F (1984) Aspergillosis of the paranasal sinuses x-ray diagnosis, histopathology, and clinical aspects. Ann Otol Rhinol Laryngol 93:251–256. https://doi.org/10.1177/000348948409300313

    Article  CAS  PubMed  Google Scholar 

  14. Qaisar Sajjad SM, Suhail Z, Ahmed R (2020) Prevalence of fungal infection in nasal polyposis - A cross-sectional study, conducted at a tertiary care hospital in Karachi. J Pak Med Assoc 70:48–52. https://doi.org/10.5455/JPMA.296507

    Article  PubMed  Google Scholar 

  15. Abreu NA, Nagalingam NA, Song Y et al (2020) Sinus microbiome diversity depletion and Corynebacterium tuberculostearicum enrichment mediates rhinosinusitis. Sci Transl Med 4:151ra124. https://doi.org/10.1126/scitranslmed.3003783

    Article  CAS  Google Scholar 

  16. Wagner Mackenzie B, West AG, Waite DW et al (2020) A novel description of the human sinus archaeome during health and chronic Rhinosinusitis. Front Cell Infect Microbiol 10:398. https://doi.org/10.3389/fcimb.2020.00398

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  17. Lai JC, Lee HS, Chen MK et al (2011) Patient satisfaction and treatment outcome of fungus ball rhinosinusitis treated by functional endoscopic sinus surgery. Eur Arch Otorhinolaryngol 268:227–230. https://doi.org/10.1007/s00405-010-1299-7

    Article  PubMed  Google Scholar 

  18. Gan W, Yang F, Meng J et al (2021) Comparing the nasal bacterial microbiome diversity of allergic rhinitis, chronic rhinosinusitis and control subjects. Eur Arch Otorhinolaryngol 278:711–718. https://doi.org/10.1007/s00405-020-06311-1

    Article  PubMed  Google Scholar 

  19. Wu Y, Chen Y, Li Q et al (2021) Tetrahydrocurcumin alleviates allergic airway inflammation in asthmatic mice by modulating the gut microbiota. Food Funct 12:6830–6840. https://doi.org/10.1039/d1fo00194a

    Article  CAS  PubMed  Google Scholar 

  20. Zhong Y, Li Y, **ao X (2014) The relationship of the expression of thymic stromal lymphopoietin in nasal polyps tissues and Th2 inflammatory response. Lin Chung Er Bi Yan Hou Tou **g Wai Ke Za Zhi 28:817–820

    CAS  Google Scholar 

  21. Salvetti E, O’Toole PW (2017) When regulation challenges innovation: the case of the genus Lactobacillus. Trends Food Sci Technol 66:S0924224416300759. https://doi.org/10.1016/j.tifs.2017.05.009

    Article  CAS  Google Scholar 

  22. De Boeck I, van den Broek MFL, Allonsius CN et al (2020) Lactobacilli Have a Niche in the Human Nose. Cell Rep 31:107674. https://doi.org/10.1016/j.celrep.2020.107674

    Article  CAS  PubMed  Google Scholar 

  23. Lagkouvardos I, Lesker TR, Hitch TCA et al (2019) Sequence and cultivation study of Muribaculaceae reveals novel species, host preference, and functional potential of this yet undescribed family. Microbiome 7:28. https://doi.org/10.1186/s40168-019-0637-2

    Article  PubMed  PubMed Central  Google Scholar 

  24. Baraniuk JN, Petrie KN, Le U et al (2005) Neuropathology in rhinosinusitis. Am J Respir Crit Care Med 171:5–11. https://doi.org/10.1164/rccm.200403-357OC

    Article  PubMed  Google Scholar 

  25. Pereira FC, Wasmund K, Cobankovic I et al (2020) Rational design of a microbial consortium of mucosal sugar utilizers reduces Clostridiodes difficile colonization. Nat Commun 11:5104. https://doi.org/10.1038/s41467-020-18928-1

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  26. Castillo Vizuete JA, Sastre J, Del Cuvillo BA et al (2019) Asthma, rhinitis, and nasal polyp multimorbidities. Arch Bronconeumol (Engl Ed) 55:146–155. https://doi.org/10.1016/j.arbres.2018.09.001

    Article  PubMed  Google Scholar 

  27. Weyand NJ (2017) Neisseria models of infection and persistence in the upper respiratory tract. Pathog Dis 75:ftx031. https://doi.org/10.1093/femspd/ftx031

    Article  CAS  Google Scholar 

  28. Adriaens LM, Alessandri R, Spörri S et al (2009) Does pregnancy have an impact on the subgingival microbiota? J Periodontol 80:72–81. https://doi.org/10.1902/jop.2009.080012

    Article  PubMed  Google Scholar 

  29. Rodríguez-Rojas L, Suarez-López A, Cantón R et al (2020) Eikenella corrodens causing deep-seated infections Six-year experience in a University Hospital in Madrid. Enferm Infecc Microbiol Clin (Engl Ed) 38(2):76–78. https://doi.org/10.1016/j.eimc.2019.04.004

    Article  PubMed  Google Scholar 

  30. Peleg AY, Hogan DA, Mylonakis E (2010) Medically important bacterial-fungal interactions. Nat Rev Microbiol 8:340–349. https://doi.org/10.1038/nrmicro2313

    Article  CAS  PubMed  Google Scholar 

  31. Kroll JS, Wilks KE, Farrant JL et al (1998) Natural genetic exchange between Haemophilus and Neisseria: intergeneric transfer of chromosomal genes between major human pathogens. Proc Natl Acad Sci U S A 95:12381–12385. https://doi.org/10.1073/pnas.95.21.12381

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Download references

Acknowledgements

The research was funded by the National Natural Science Foundation of China (grant award number 81070768) and Wu Jie** Clinical Research Special Assistance Fund (grant award number 320.6750); National Key Research & Development Program of China (2017YFC0112500), Bei**g Municipal Administration of Hospitals’ Mission Plan (SML20150201).

Ethical Approval

Ethical issues (Including plagiarism, informed consent, misconduct, data fabrication and/or falsification, double publication and/or submission, redundancy, etc.) have been completely observed by the authors.

Author information

Author notes

  1. Pengyu Shi and Hongzheng Wei have contributed equally to this research.

Authors and Affiliations

  1. Department of Otolaryngology Head and Neck Surgery, Key Laboratory of Otolaryngology Head and Neck Surgery, Ministry of Education, Bei**g TongRen Hospital, Capital Medical University, Bei**g, 100730, People’s Republic of China

    Pengyu Shi, Hongzheng Wei, **n Liu, Shouxiang Dong, Shuai He, Yun Zeng, Ting Yang, Chengyao Liu & Yunchuan Li

  2. Key Laboratory of Otolaryngology Head and Neck Surgery, Ministry of Education, Capital Medical University, Bei**g, China

    Pengyu Shi, Hongzheng Wei, **n Liu, Shouxiang Dong, Shuai He, Yun Zeng, Ting Yang, Chengyao Liu & Yunchuan Li

  3. Department of Neurology, Bei**g Tongren Hospital Capital Medical University, Bei**g, 100730, China

    Pengyu Shi

Authors
  1. Pengyu Shi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hongzheng Wei
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. **n Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Shouxiang Dong
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Shuai He
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Yun Zeng
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Ting Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Chengyao Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Yunchuan Li
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Yunchuan Li.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Shi, P., Wei, H., Liu, X. et al. The Nasal Bacteria Microbiome Comparison Among Fungal Ball Sinusitis, Chronic Sinusitis with Polyps. Indian J Microbiol 63, 120–128 (2023). https://doi.org/10.1007/s12088-023-01062-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12088-023-01062-z

Keywords

Search

Navigation