Log in

Metagenomics at grass roots

  • General Article
  • Published:
Resonance Aims and scope Submit manuscript

Abstract

Metagenomics is a robust, interdisciplinary approach for studying microbial community composition, function, and dynamics. It typically involves a core of molecular biology, microbiology, ecology, statistics, and computational biology. Exciting outcomes anticipated from these studies include unraveling of complex interactions that characterize the ecological milieu of microbial communities. Diverse habitats from which metagenomes have been reported include human guts, caterpillar guts, thermal vents in oceans, ore deposits, polar caps, and even soils that adhere to plant roots. Knowledge generated from metagenomic projects has tremendous potential to beneflt human health, agriculture, and ecosystem functions. This article provides a brief history of technical advances in metagenomics, including DNA sequencing methods, and some case studies. A speciflc example is provided of microbial metagenomes found at the roots of native grass species (family Poaceae) that can grow on degraded lands undergoing revegetation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

Suggested Reading

  1. J Handelsman, MR Rondon, S F Brady, J Clardy, and RM Goodman, Molecular biological access to the chemistry of unknown soil microbes: a new frontier for natural products, Chemistry and Biology, Vol. 5, No. 10, pp. R245–249, 1998.

    Article  Google Scholar 

  2. P Hugenholtz and G W Tyson, Metagenomics, Nature, Vol. 455, pp. 481–483, 2008.

    Article  Google Scholar 

  3. P D Schloss and J Handelsman, Biotechnological prospects from metagenomics, Current Opinion in Biotechnology, Vol. 14, pp. 303–310, 2003.

    Article  Google Scholar 

  4. T Thomas, J Gilbert and F Meyer, Metagenomicsa guide from sampling to data analysis, Microbial Informatics and Experimentation, Vol. 2, No. 3, 2012. doi:10.1186/2042-5783-2-3.

  5. R D Bardgett and D A Wardle, Aboveground-Below-ground linkages: Biotic Interactions, Ecosystem processes and Global change, Chapter 3, pp. 62–110, 2010.

    Google Scholar 

  6. A C Howe, J K Jansson, S A Malfatti, S G Tringe, J M Tiedje and C T Brown, Tackling soil diversity with the assembly of large, complex metagenomes, Proceeding of the National Academy of Sciences (USA), Vol. 111, pp. 4904–4909, 2014.

  7. J Nesme et al., Back to the future of soil metagenomics, Frontiers in Microbiology, Vol. 7, No. 73, 2016.

  8. V K Choudhary, Microbial diversity at Iron ore mined-out sites in Odisha and its signiflcance in Ecological Restoration, PhD thesis, University of Delhi, 2016.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Sudeshna Mazumdar-Leighton.

Additional information

Sudeshna Mazumdar-Leighton is a molecular biologist at Delhi University. Her research interests are plant-biotic environment interactions, relevant to marginalized communities.

Vivek K Choudhary has just completed a PhD dissertation on restoration ecology of iron-ore mine sites in Odisha. He is interested in plant community dynamics, revegetation, and soil metagenomics.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Mazumdar-Leighton, S., Choudhary, V.K. Metagenomics at grass roots. Reson 22, 291–301 (2017). https://doi.org/10.1007/s12045-017-0461-6

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12045-017-0461-6

Keywords

Navigation