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Downregulated expression of PTK6 is correlated with poor survival in esophageal squamous cell carcinoma

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Abstract

To investigate the clinical prognostic value of protein tyrosine kinase 6 (PTK6) in patients with esophageal squamous cell carcinoma (ESCC), quantitative RT-PCR and Western blotting were utilized to measure the mRNA and protein expression levels of PTK6 in 29 and eight pairs of ESCC and peritumoral normal esophageal tissues, respectively. Furthermore, the expression of PTK6 protein in 210 ESCCs was examined with immunohistochemistry (IHC), and its clinical value was analyzed using Kaplan–Meier plots and the Cox proportional hazards regression model. The results found that the expression levels of both PTK6 mRNA and protein in ESCC tissues were significantly lower than those in peritumoral normal esophageal tissues. Regarding the IHC analysis of ESCC, the cytoplasmic expression of PTK6 was significantly correlated with tumor grade (P < 0.001). Compared with patients with low PTK6 expression, ESCC patients with overexpression of PTK6 displayed preferable disease-free survival (DFS) and overall survival (OS) (P < 0.001 and P = 0.001, respectively), especially in stage II disease (P = 0.002 and P = 0.021, respectively). PTK6 was evaluated as an independent prognostic factor for ESCC using multivariate Cox regression analysis. All data demonstrated that the expression level of PTK6 is an independent prognostic factor in ESCCs. Low expression of PTK6 is correlated with poor DFS and OS in ESCCs.

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Acknowledgments

This study was supported by the National Nature Science Foundation of China (Grant Number 81372568).

Conflict of interest

We declare that we have no conflict of interest.

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Authors and Affiliations

  1. State Key Laboratory of Oncology in South China, Cancer Center, Sun Yat-sen University, No. 651, Dongfeng Road East, Guangzhou, 510060, Guangdong Province, China

    You-Fang Chen, Gang Ma, Xun Cao, Zhi-Liang Huang, Mu-Sheng Zeng & Zhe-Sheng Wen

  2. The Sixty Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510655, Guangdong Province, China

    You-Fang Chen

  3. Department of Thoracic Oncology, Cancer Center, Sun Yat-sen University, No. 651, Dongfeng Road East, Guangzhou, 510060, Guangdong Province, China

    Zhi-Liang Huang & Zhe-Sheng Wen

  4. Department of Intensive Care Medicine, Cancer Center, Sun Yat-sen University, Guangzhou, 510060, Guangdong Province, China

    Xun Cao

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  1. You-Fang Chen
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  2. Gang Ma
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  3. Xun Cao
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Correspondence to Zhe-Sheng Wen.

Additional information

You-Fang Chen and Gang Ma contributed equally to the work as first author.

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Chen, YF., Ma, G., Cao, X. et al. Downregulated expression of PTK6 is correlated with poor survival in esophageal squamous cell carcinoma. Med Oncol 31, 317 (2014). https://doi.org/10.1007/s12032-014-0317-9

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