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Immobilization of Lipase from Candida antarctica B (CALB) by Sol–Gel Technique Using Rice Husk Ash as Silic Source and Ionic Liquid as Additive

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Abstract 

This work presents the immobilization in situ of commercial lipase from Candida antarctica B (CALB) by the sol–gel technique (xerogel) using silica from rice husk ash (RHA) as a source of silicon. It was used the Ionic Liquid (IL) 1-octyl-3-methylimidazolium bromide (C8MI.Br) as additive. The immobilized derivatives were characterized per SEM, XRD, and per method BET. The enzymatic activity of xerogels was evaluated with different tests, these being the reactional thermal analysis, immobilization yield, and operational and storage stability. The XDR showed that the obtained xerogels have halos in the region between 15 and 35° (2θ) what characterizes it as amorphous materials. The SEM analysis of xerogel shows irregular particles with dimensions less than 20 μm. The immobilized presented an esterification activity (EA) with 263.2 and 213.8 U/g, with and without IL, respectively, higher than the free enzyme (169.6 U/g). The immobilized, with and without IL, presented a significant improvement in the activity performance in relation to free enzyme for the three reactional temperatures (40, 60, and 80 °C) evaluated. The operational stability demonstrated that is possible to use xerogel without ionic liquid for 17 recycles and 21 recycles in IL presence. This methodology allows the preparation of new highly active and selective enzyme catalysts using the rice husk ash as a source of silicon, and the ionic liquid [C8MI]Br as additive. Furthermore, the new materials can provide greater viability in the processes, ensuring longer catalyst life.

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Data Availability

The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

The authors thank to URI Erechim, National Council for Scientific and Technological Development (CNPq), Coordination for the Improvement of Higher Education Personnel (CAPES) and Research Support Foundation of the State of Rio Grande do Sul (FAPERGS).

Funding

This work was financially supported by CAPES, FAPERGS, and CNPq.

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Authors and Affiliations

  1. Department of Food and Chemical Engineering, URI – Erechim, Sete de Setembro Av, Erechim, RS, 162199709-910, Brazil

    Josieli Fátima Vesoloski, Adriele Sabrina Todero, Ricardo Jorge Macieski, Fabiana de Oliveira Pereira, Rogério Marcos Dallago & Marcelo Luis Mignoni

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  1. Josieli Fátima Vesoloski
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  2. Adriele Sabrina Todero
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Contributions

J.F. Vesoloski: Investigation & Writing.

A.S. Todero: Investigation & Writing

R.J. Macieski: Investigation & Writing

F.O. Pereira: Investigation & Writing

R.M. Dallago: Formal Analysis, Writing, Review & Editing

M.L. Mignoni: Supervision, Formal Analysis, Writing, Review & Editing

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Correspondence to Marcelo Luis Mignoni.

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Highlights

•Silicon from rice husk ash was successfully used for in situ immobilization of CALB in xerogel.

•CALB immobilized in sol-gel presented 21 recycles with IL C8MI.Br and 17 without IL C8MI.Br.

•Enzymatic activity remained stable for 96 days; however, the tests just with enzyme reached 126 days.

•The supports were able to catalyze the esterification reaction at 40, 60, and 80 °C.

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Vesoloski, J.F., Todero, A.S., Macieski, R.J. et al. Immobilization of Lipase from Candida antarctica B (CALB) by Sol–Gel Technique Using Rice Husk Ash as Silic Source and Ionic Liquid as Additive. Appl Biochem Biotechnol 194, 6270–6286 (2022). https://doi.org/10.1007/s12010-022-04096-z

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