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In Vitro Angiogenic Properties of Plasmid DNA Encoding SDF-1α and VEGF165 Genes

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Abstract

The stromal-derived factor-1 alpha (SDF-1α) and vascular endothelial growth factor (VEGF) play an important role in angiogenesis and exert a significant trophic function. SDF-1α is a chemoattractant for endothelial progenitor cells derived from bone marrow and promotes new blood vessel formation. VEGF regulates all types of vascular growth, stimulates angiogenesis, and is involved in the induction of lymphangiogenesis. The possibility of using these growth factors for regenerative medicine is currently under investigation. The angiogenic potential of a pBud-SDF-1α-VEGF165 bicistronic plasmid construct which simultaneously encodes VEGF165 and SDF-1α genes cDNA was evaluated in this study. The conditioned medium collected from HEK293T cells transfected with the pBud-SDF-1α-VEGF165 plasmid was shown to stimulate the formation of capillary-like structures by human umbilical vein-derived endothelial cells (HUVEC) on Matrigel and to increase the proliferative activity of these cells in vitro. Thus, the pBud-SDF-1α-VEGF165 plasmid exhibits angiogenic properties in cell cultures in vitro. As interest in the development of non-viral techniques for regenerative medicine increases, this plasmid which simultaneously expresses VEGF165 and SDF-1α may provide a platform for advanced methods of stimulating therapeutic angiogenesis.

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Conflict of Interest

The authors declare that they have no conflict of interest.

Funding

The work was funded by the Human Stem Cells Institute and performed according to the Russian Government Program of Competitive Growth of the Kazan Federal University. AR was supported by the state assignment 20.5175.2017/6.7 of the Ministry of Education and Science of the Russian Federation and the President of the Russian Federation grant НШ-3076.2018.4. IS and VS were supported by the Russian Foundation for Basic Research grant 16-04-01567.

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Authors and Affiliations

  1. Kazan Federal University, Kazan, Russia

    Valeriya V. Solovyeva, Daria S. Chulpanova, Leysan G. Tazetdinova, Ilnur I. Salafutdinov & Albert A. Rizvanov

  2. Human Stem Cells Institute, Moscow, Russia

    Artur A. Isaev

  3. Histograft, LLC, Moscow, Russia

    Ilia Y. Bozo

  4. Federal Medical Biophysical Center, FMBA of Russia, Moscow, Russia

    Ilia Y. Bozo

  5. Ryazan State Medical University, Ryazan, Russia

    Roman V. Deev

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  1. Valeriya V. Solovyeva
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Correspondence to Albert A. Rizvanov.

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The protocol was approved by the Biomedicine Ethics Expert Committee of the Kazan Federal University (No. 3, 23.03.2017). Written informed consent was obtained from the donors.

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Solovyeva, V.V., Chulpanova, D.S., Tazetdinova, L.G. et al. In Vitro Angiogenic Properties of Plasmid DNA Encoding SDF-1α and VEGF165 Genes. Appl Biochem Biotechnol 190, 773–788 (2020). https://doi.org/10.1007/s12010-019-03128-5

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