Abstract
Pectin methyl esterase (PME) is responsible for the destabilization of clouds in sweet orange juice. The study aims to optimize pulsed light (PL) and ultrasound (US) treatments for the maximum inactivation of PME in sweet orange juice. A central composite design was used for PL treatments (2.5 -2.9 kV for 200–300 s) and US treatments (40- 120 W for 2–10 min). The numerical optimization predicted 98.5% inactivation of PME in the juice after 283 s PL exposure at 2.84 kV (2711 J/cm2). US optimized condition was 108 W for 9 min with 82.5% PME inactivation. The first-order rate constants for PL-induced PME inactivation varied from 0.0033 to 0.0159 s−1, whereas, for the US, the range was 0.00126 to 0.0015 s−1. The impact of PL and US treatments in series on various quality attributes of the juice was assessed. PL exposure at 2.4 kV for 180 s [1278 J/cm2; the highest intensity of PL] followed by US treatment for 360 s at 80 W [1.14 W/mL; the highest intensity of US] resulted in 98.3% PME inactivation, > 5-log reduction in natural microbiota, 5% loss in vitamin C, 1.7% loss in antioxidant capacity, and 99.4% retention in phenolics in the juice. The sequence of PL or US had minimal impact on enzyme inactivation and bioactive degradation in the juice. The PL-optimized processing also led to more than 5.0 log reductions in natural microflora. However, the preliminary sensory acceptance, color difference, and bioactive retention of the sample treated at the highest intensity of PL+US were superior to the PL-optimized juice.
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References
Aadil, R. M., Zeng, X. A., Han, Z., & Sun, D. W. (2013). Effects of ultrasound treatments on quality of grapefruit juice. Food Chemistry, 141(3), 3201–3206. https://doi.org/10.1016/j.foodchem.2013.06.008
Abid, M., Jabbar, S., Wu, T., Hashim, M. M., Hu, B., Lei, S., Zhang, X., & Zeng, X. (2013). Effect of ultrasound on different quality parameters of apple juice. Ultrasonics Sonochemistry, 20(5), 1182–1187. https://doi.org/10.1016/j.ultsonch.2013.02.010
Adekunte, A. O., Tiwari, B. K., Cullen, P. J., Scannell, A. G. M., & O’Donnell, C. P. (2010). Effect of sonication on color, ascorbic acid and yeast inactivation in tomato juice. Food Chemistry, 122(3), 500–507. https://doi.org/10.1016/j.foodchem.2010.01.026
Aschoff, J. K., Knoblauch, K., Hüttner, C., Vásquez-Caicedo, A. L., Carle, R., & Schweiggert, R. M. (2016). Non-thermal pasteurization of orange (Citrus sinensis (L.) Osbeck) juices using continuous pressure change technology (PCT): a proof-of-concept. Food and Bioprocess Technology, 9(10), 1681–1691. https://doi.org/10.1007/s11947-016-1754-6
Azzini, E., Venneria, E., Ciarapica, D., Foddai, M. S., Intorre, F., Zaccaria, M., Maiani, F., Palomba, L., Barnaba, L., Tubili, C., Maiani, G., & Polito, A. (2017). Effect of red orange juice consumption on body composition and nutritional status in overweight/obese female: A pilot study. Oxidative Medicine and Cellular Longevity, 2017, 1–9. https://doi.org/10.1155/2017/1672567
Baldwin, E. A., Bai, J., Plotto, A., Cameron, R., Luzio, G., Narciso, J., & Ford, B. L. (2012). Effect of extraction method on quality of orange juice: hand-squeezed, commercial-fresh squeezed and processed. Journal of the Science of Food and Agriculture, 92(10), 2029–2042. https://doi.org/10.1002/jsfa.5587
Basak, S., & Chakraborty, S. (2022). The potential of non-thermal techniques to achieve enzyme inactivation in fruit products. Trends in Food Science & Technology, 123, 114–129. https://doi.org/10.1016/j.tifs.2022.03.008
Bevilacqua, A., Speranza, B., Campaniello, D., Sinigaglia, M., & Corbo, M. R. (2014). Inactivation of spoiling yeasts of fruit juices by pulsed ultrasound. Food and Bioprocess Technology, 7(8), 2189–2197. https://doi.org/10.1007/s11947-013-1178-5
Bhagat, B., & Chakraborty, S. (2022). Potential of pulsed light treatment to pasteurize pomegranate juice: microbial safety, enzyme inactivation, and phytochemical retention. LWT, 159, 113215. https://doi.org/10.1016/j.lwt.2022.113215
Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72(1–2), 248–254. https://doi.org/10.1016/0003-2697(76)90527-3
Caminiti, I. M., Noci, F., Morgan, D. J., Cronin, D. A., & Lyng, J. G. (2012). The effect of pulsed electric fields, ultraviolet light or high intensity light pulses in combination with manothermosonication on selected physico-chemical and sensory attributes of an orange and carrot juice blend. Food and Bioproducts Processing, 90(3), 442–448. https://doi.org/10.1016/j.fbp.2011.11.006
Chakraborty, S., Ghag, S., Bhalerao, P. P., & Gokhale, J. S. (2020). The potential of pulsed light treatment to produce enzymatically stable Indian gooseberry (Emblica officinalis Gaertn.) juice with maximal retention in total phenolics and vitamin C. Journal of Food Processing and Preservation, 44(12), 1–12. https://doi.org/10.1111/jfpp.14932
Chakraborty, S., Mahale, S., Dhar, R., & Basak, S. (2022). Development of a mixed fruit beverage and pulsed light treatment thereof to obtain a microbially safe and enzymatically stable product. Food Bioscience, 45, 101508. https://doi.org/10.1016/j.fbio.2021.101508
Chakraborty, S., Rao, P. S., & Mishra, H. N. (2015). Empirical model based on Weibull distribution describing the destruction kinetics of natural microbiota in pineapple (Ananas comosus L.) puree during high-pressure processing. International Journal of Food Microbiology, 211, 117–127. https://doi.org/10.1016/j.ijfoodmicro.2015.06.017
Croak, S., & Corredig, M. (2006). The role of pectin in orange juice stabilization: Effect of pectin methylesterase and pectinase activity on the size of cloud particles. Food Hydrocolloids, 20(7), 961–965. https://doi.org/10.1016/j.foodhyd.2005.10.016
Cserhalmi, Z., Sass-Kiss, Á., Tóth-Markus, M., & Lechner, N. (2006). Study of pulsed electric field treated citrus juices. Innovative Food Science and Emerging Technologies, 7(1–2), 49–54. https://doi.org/10.1016/j.ifset.2005.07.001
Dhar, R., & Chakraborty, S. (2020). Influence of voltage and distance on quality attributes of mixed fruit beverage during pulsed light treatment and kinetic modelling. Journal of Food Process Engineering, 43(11), 1–14. https://doi.org/10.1111/jfpe.13517
Dhar, R., Basak, S., & Chakraborty, S. (2022). Pasteurization of fruit juices by pulsed light treatment: A review on the microbial safety, enzymatic stability, and kinetic approach to process design. Comprehensive Reviews in Food Science and Food Safety, 21(1), 499–540. https://doi.org/10.1111/1541-4337.12864
Ellerbee, L., & Wicker, L. (2011). Calcium and pH influence on orange juice cloud stability. Journal of the Science of Food and Agriculture, 91(1), 171–177. https://doi.org/10.1002/jsfa.4169
Ferrario, M., & Guerrero, S. (2016). Effect of a continuous flow-through pulsed light system combined with ultrasound on microbial survivability, color and sensory shelf life of apple juice. Innovative Food Science & Emerging Technologies, 34, 214–224. https://doi.org/10.1016/j.ifset.2016.02.002
Ferrario, M., & Guerrero, S. (2017). Impact of a combined processing technology involving ultrasound and pulsed light on structural and physiological changes of Saccharomyces cerevisiae KE 162 in apple juice. Food Microbiology, 65, 83–94. https://doi.org/10.1016/j.fm.2017.01.012
Ferrario, M., Alzamora, S. M., & Guerrero, S. (2013). Inactivation kinetics of some microorganisms in apple, melon, orange and strawberry juices by high intensity light pulses. Journal of Food Engineering, 118(3), 302–311. https://doi.org/10.1016/j.jfoodeng.2013.04.007
Ferrario, M., Alzamora, S. M., & Guerrero, S. (2015). Study of the inactivation of spoilage microorganisms in apple juice by pulsed light and ultrasound. Food Microbiology, 46, 635–642. https://doi.org/10.1016/j.fm.2014.06.017
Fonteles, T. V., Barroso, M. K. D. A., Alves Filho, E. D. G., Fernandes, F. A. N., & Rodrigues, S. (2021). Ultrasound and ozone processing of cashew apple juice: effects of single and combined processing on the juice quality and microbial stability. Processes, 9(12), 2243. https://doi.org/10.3390/pr9122243
Gómez-López, V. M., Orsolani, L., Martínez-Yépez, A., & Tapia, M. S. (2010). Microbiological and sensory quality of sonicated calcium-added orange juice. LWT - Food Science and Technology, 43(5), 808–813. https://doi.org/10.1016/j.lwt.2010.01.008
Guerrouj, K., Sánchez-Rubio, M., Taboada-Rodríguez, A., Cava-Roda, R. M., & Marín-Iniesta, F. (2016). Sonication at mild temperatures enhances bioactive compounds and microbiological quality of orange juice. Food and Bioproducts Processing, 99, 20–28. https://doi.org/10.1016/j.fbp.2016.03.007
Hagerman, A. E., & Austin, P. J. (1986). Continuous spectrophotometric assay for plant pectin methyl esterase. Journal of Agricultural and Food Chemistry, 34(3), 440–444. https://doi.org/10.1021/jf00069a015
Khandpur, P., & Gogate, P. R. (2015). Understanding the effect of novel approaches based on ultrasound on sensory profile of orange juice. Ultrasonics Sonochemistry, 27, 87–95. https://doi.org/10.1016/j.ultsonch.2015.05.001
Klavons, J. A., Bennett, R. D., & Vannier, S. H. (1991). Nature of the protein constituent of commercial orange juice cloud. Journal of Agricultural and Food Chemistry, 39(9), 1545–1548. https://doi.org/10.1021/jf00009a001
Klavons, J. A., Bennett, R. D., & Vannier, S. H. (1994). Physical/chemical nature of pectin associated with commercial orange juice cloud. Journal of Food Science, 59(2), 399–401. https://doi.org/10.1111/j.1365-2621.1994.tb06976.x
Koshani, R., Ziaee, E., Niakousari, M., & Golmakani, M. T. (2015). Optimization of thermal and thermosonication treatments on pectin methyl esterase inactivation of sour orange juice (Citrus aurantium). Journal of Food Processing and Preservation, 39(6), 567–573. https://doi.org/10.1111/jfpp.12262
Kwaw, E., Ma, Y., Tchabo, W., Apaliya, M. T., Sackey, A. S., Wu, M., & **ao, L. (2018). Impact of ultrasonication and pulsed light treatments on phenolics concentration and antioxidant activities of lactic-acid-fermented mulberry juice. LWT, 92, 61–66. https://doi.org/10.1016/j.lwt.2018.02.016
Ladaniya, M. S., Marathe, R. A., Murkute, A. A., Huchche, A. D., Das, A. K., George, A., & Kolwadkar, J. (2021). Response of Nagpur mandarin (Citrus reticulata Blanco) to high density planting systems. Scientific Reports, 11(1), 1–11. https://doi.org/10.1038/s41598-021-89221-4
Muñoz, A., Caminiti, I. M., Palgan, I., Pataro, G., Noci, F., Morgan, D. J., Cronin, D. A., Whyte, P., Ferrari, G., & Lyng, J. G. (2012). Effects on Escherichia coli inactivation and quality attributes in apple juice treated by combinations of pulsed light and thermosonication. Food Research International, 45(1), 299–305. https://doi.org/10.1016/j.foodres.2011.08.020
Muñoz, A., Palgan, I., Noci, F., Morgan, D. J., Cronin, D. A., Whyte, P., & Lyng, J. G. (2011). Combinations of high intensity light pulses and thermosonication for the inactivation of Escherichia coli in orange juice. Food Microbiology, 28(6), 1200–1204. https://doi.org/10.1016/j.fm.2011.04.005
Nunes, B. V., da Silva, C. N., Bastos, S. C., & de Souza, V. R. (2022). Microbiological inactivation by ultrasound in liquid products. Food and Bioprocess Technology, 15(10), 2185–2209. https://doi.org/10.1007/s11947-022-02818-z
Oliveira, A. F. A., Mar, J. M., Santos, S. F., da Silva Júnior, J. L., Kluczkovski, A. M., Bakry, A. M., Bezerra, J. D. A., Nunomura, R. D. C. S., Sanches, E. A., & Campelo, P. H. (2018). Non-thermal combined treatments in the processing of açai (Euterpe oleracea) juice. Food Chemistry, 265, 57–63. https://doi.org/10.1016/j.foodchem.2018.05.081
Ordóñez-Santos, L. E., Martínez-Girón, J., & Arias-Jaramillo, M. E. (2017). Effect of ultrasound treatment on visual color, vitamin C, total phenols, and carotenoids content in Cape gooseberry juice. Food Chemistry, 233, 96–100. https://doi.org/10.1016/j.foodchem.2017.04.114
Orlowska, M., Koutchma, T., Grapperhaus, M., Gallagher, J., Schaefer, R., & Defelice, C. (2013). Continuous and pulsed ultraviolet light for nonthermal treatment of liquid foods. Part 1: effects on quality of fructose solution, apple juice, and milk. Food and Bioprocess Technology, 6(6), 1580–1592. https://doi.org/10.1007/s11947-012-0779-8
Pellicer, J. A., Navarro, P., & Gómez-López, V. M. (2020). Pectin methylesterase inactivation by pulsed light. Innovative Food Science and Emerging Technologies, 62, 102366. https://doi.org/10.1016/j.ifset.2020.102366
Raviyan, P., Zhang, Z., & Feng, H. (2005). Ultrasonication for tomato pectinmethylesterase inactivation: effect of cavitation intensity and temperature on inactivation. Journal of Food Engineering, 70(2), 189–196. https://doi.org/10.1016/j.jfoodeng.2004.09.028
Ropartz, D., & Ralet, M. C. (2020). Pectin structure. In V. Kontogiorgos (Ed.), Pectin: technological and physiological properties. Cham: Springer. https://doi.org/10.1007/978-3-030-53421-9_2
Salazar-Zúñiga, M. N., Lugo-Cervantes, E., Rodríguez-Campos, J., Sanchez-Vega, R., Rodríguez-Roque, M. J., & Valdivia-Nájar, C. G. (2022). Pulsed light processing in the preservation of juices and fresh-cut fruits: a review. Food and Bioprocess Technology, 16, 510–525. https://doi.org/10.1007/s11947-022-02891-4
Shaik, L., & Chakraborty, S. (2022a). Effect of pH and total fluence on microbial and enzyme inactivation in sweet lime (Citrus limetta) juice during pulsed light treatment. Journal of Food Processing and Preservation, 46(8), e16749. https://doi.org/10.1111/jfpp.16749
Shaik, L., & Chakraborty, S. (2022b). Ultrasound processing of sweet lime juice: Effect of matrix pH on microbial inactivation, enzyme stability, and bioactive retention. Journal of Food Process Engineering, e14231. https://doi.org/10.1111/jfpe.14231
Terefe, N. S., Gamage, M., Vilkhu, K., Simons, L., Mawson, R., & Versteeg, C. (2009). The kinetics of inactivation of pectin methylesterase and polygalacturonase in tomato juice by thermosonication. Food Chemistry, 117(1), 20–27. https://doi.org/10.1016/j.foodchem.2009.03.067
Tiwari, B. K., Muthukumarappan, K., O’Donnell, C. P., & Cullen, P. J. (2009). Inactivation kinetics of pectin methylesterase and cloud retention in sonicated orange juice. Innovative Food Science and Emerging Technologies, 10(2), 166–171. https://doi.org/10.1016/j.ifset.2008.11.006
Tomadoni, B., Cassani, L., Viacava, G., Moreira, M. D. R., & Ponce, A. (2017). Effect of ultrasound and storage time on quality attributes of strawberry juice. Journal of Food Process Engineering, 40(5), 1–8. https://doi.org/10.1111/jfpe.12533
Valero, M., Recrosio, N., Saura, D., Muñoz, N., Martí, N., & Lizama, V. (2007). Effects of ultrasonic treatments in orange juice processing. Journal of Food Engineering, 80(2), 509–516. https://doi.org/10.1016/j.jfoodeng.2006.06.009
Vollmer, K., Chakraborty, S., Bhalerao, P. P., Carle, R., Frank, J., & Steingass, C. B. (2020). Effect of pulsed light treatment on natural microbiota, enzyme activity, and phytochemical composition of pineapple (Ananas comosus [L.] Merr.) juice. Food and Bioprocess Technology, 13(7), 1095–1109. https://doi.org/10.1007/s11947-020-02460-7
Wormit, A., & Usadel, B. (2018). The multifaceted role of pectin methylesterase inhibitors (PMEIs). International Journal of Molecular Sciences, 19(10), 2878. https://doi.org/10.3390/ijms19102878
Yolmeh, M., & Jafari, S. M. (2017). Applications of response surface methodology in the food industry processes. Food and Bioprocess Technology, 10(3), 413–433. https://doi.org/10.1007/s11947-016-1855-2
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The research is funded by the Ministry of Food Processing Industries, Govt of India, under the grant number: Q-11/42/2018-R&D.
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Padmanav Sahoo: Methodology, Formal Analysis, Investigation, Validation, Resources, Writing- Reviewing and Editing. Snehasis Chakraborty: Conceptualization, Supervision, Writing- Original draft preparation, Revision–Reviewing and Editing, Funding Acquisition.
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Sahoo, P., Chakraborty, S. Influence of Pulsed Light, Ultrasound, and Series Treatments on Quality Attributes, Pectin Methyl Esterase, and Native Flora Inactivation in Sweet Orange Juice (Citrus sinensis L. Osbeck). Food Bioprocess Technol 16, 2095–2112 (2023). https://doi.org/10.1007/s11947-023-03042-z
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DOI: https://doi.org/10.1007/s11947-023-03042-z