Abstract
Columbia-0 (Col-0) appears to be less tolerant to cadmium (Cd) than the Wassilewskija (Ws) ecotype that exhibits the full Heavy Metal ATPase3 (HMA3) coding sequence. However, the physiological and molecular mechanisms of HMA3 encoded by point mutation genes in Col-0 remain unknown. In this study, we investigate whether the different metal-related phenotype observed in Col-0 (with HMA3 mutation) when compared to that of Ws (functional HMA3) is a result only of the HMA3 mutation. This investigation was carried out with a further study using plant materials as follows: Ws and Col-0 ecotypes, two HMA3 (Ws) overexpressing lines in Col-0, hma3 knock-out line in Ws. The results indicate that the Col-0 and hma3 mutant in Ws were less tolerant to Cd and Zn because HMA3 has lost the function of sequestration of Cd and Zn into the root vacuoles, thereby readily translocating Cd and Zn to the aerial parts. In addition, the root-to-shoot metal translocation rates of the Ws- and HMA3-overexpressing lines were lower than those of the Col-0 and hma3 mutants. These results indicate that HMA3 is important for the Cd and Zn detoxification in Arabidopsis.
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Acknowledgements
This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF), funded by the Ministry of Education (2014R1A6A3A01059173 and 20141A1A4A01009621). This work was also supported by a grant from the Next-Generation BioGreen 21 Program (SSAC, Grant#: PJ01108102), Rural Development Administration, Republic of Korea.
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In this study, Won Park contributed to experimental conception, design, and writing the article. Dr. Sung-Ju Ahn participated in designing the study and revised the manuscript. All authors contributed to the article to be completed.
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Park, W., Ahn, SJ. HMA3 is a key factor for differences in Cd- and Zn-related phenotype between Arabidopsis Ws and Col-0 ecotypes. Plant Biotechnol Rep 11, 209–218 (2017). https://doi.org/10.1007/s11816-017-0447-6
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DOI: https://doi.org/10.1007/s11816-017-0447-6