Abstract
Sinojackia microcarpa is a kind of endangered species distributed in several places in China. In this study, we sequenced the chloroplast genome of S. microcarpa using the Illumina NovaSeq PE150 platform. The generated chloroplast genome sequence was 158,740 bp in length containing a pair of identical inverted repeats (IR) regions of 26,090 bp separated by a large single-copy (LSC) region of 88,006 bp and a small single-copy (SSC) region of 18,554 bp. The chloroplast genome contained 85 protein-coding genes, 37 transfer RNA (tRNA) and eight ribosomal RNA (rRNA) genes.The cp genome comparison between different species of Sinojackia revealed that the complete chloroplast genomes were very conserved between different species. Different kinds of SSRs and oligonucleotide repeats were identified in our study, which could be used to identify species or genus. Further, the phylogenetic trees using complete chloroplast genomes, trnL, matK and rbcL genes sequences constructed a genetic relationship between Styracaceae, rbcL was proved to be a suitable DNA barcoding marker used to analyze the phylogenetic relationship of Styracaceae.
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Abbreviations
- LSC:
-
large single copy
- SSC:
-
small single copy
- IR:
-
inverted repeat regions
- SSR:
-
simple sequence repeat
- MAFFT:
-
Multiple Alignment using Fast Fourier Transform
- SNPs:
-
single nucleotide polymorphisms
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Acknowledgments
We thank Mr. Guohua **a and **yang Ye for their assistance during leaves collection.
Data availability statement
The data that support the findings of this study have been deposited in the NCBI database [GenBank accession:MW421726.1] (http://www.ncbi.nlm.nih.gov/).The associated **SRA** and **Bio-Sample** numbers are PRJNA727626 and SAMN19027281 respectively.
Funding
This work was supported by the Basic Public Welfare Research Projects of Zhejiang province, China [LGN21C160015] and Research start-up Project of Zhejiang Shuren University [2020R004].
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Zhong, T., Zhuo, J., Chen, D. et al. Complete chloroplast genome sequence of Sinojackia microcarpa (Styracaceae): comparative and phylogenetic analysis. Biologia 76, 3891–3900 (2021). https://doi.org/10.1007/s11756-021-00913-8
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DOI: https://doi.org/10.1007/s11756-021-00913-8