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Quantitative Analysis of TAG in Oils Using Lithium Cationization and Direct-Infusion ESI Tandem Mass Spectrometry

  • Original Paper
  • Published:
Journal of the American Oil Chemists' Society

Abstract

This study was undertaken to determine whether triple-stage mass spectrometry (MS3) could be employed to obtain quantitative and regioisomeric data from complex oil samples without the need for a chromatographic step in the analysis protocol. Lithium-7 trifluoroacetate and electrospray ionization were used to form lithium adducts of the triacylglycerols (TAG) in a fish oil sample. The first-generation precursor ion was the lithium-TAG adduct, the second-generation precursor ion was formed by loss of a neutral acid side chain in the first fragmentation. The ions used for analysis were formed in the second fragmentation by loss of the lactones of the acid side chains remaining after the first fragmentation. This analysis scheme provided quantitative and regioisomeric data without interference from TAG in the sample other than TAG with the same acyl carbon number, one more double bond, and two acyl side chains in common with the analyte. Even in this case a majority of the interferences could be estimated and compensated. Analysis of synthetic samples containing the fish oil matrix indicated that both absolute and relative quantitative data could be obtained with average errors of approximately 5 %. The method proved well suited to routine analyses of complex oil samples.

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Acknowledgments

The authors wish to acknowledge the helpful discussions with Prof. J. S. Grossert during the preparation of this manuscript.

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Author notes

  1. Lisandra Cubero Herrera

    Present address: Canadian Food Inspection Agency, 1992 Agency Dr., Dartmouth, NS, B3B 1Y9, Canada

Authors and Affiliations

  1. Department of Chemistry, Dalhousie University, Halifax, NS, B3H 4R2, Canada

    Louis Ramaley

  2. National Research Council Canada, 1411 Oxford St., Halifax, NS, B3H 3Z1, Canada

    Louis Ramaley, Lisandra Cubero Herrera & Jeremy E. Melanson

  3. Measurement Science and Standards, National Research Council Canada, 1200 Montreal Rd., Ottawa, ON, K1A 0R6, Canada

    Jeremy E. Melanson

Authors
  1. Louis Ramaley
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Corresponding author

Correspondence to Louis Ramaley.

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Ramaley, L., Herrera, L.C. & Melanson, J.E. Quantitative Analysis of TAG in Oils Using Lithium Cationization and Direct-Infusion ESI Tandem Mass Spectrometry. J Am Oil Chem Soc 92, 323–334 (2015). https://doi.org/10.1007/s11746-015-2604-9

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  • DOI: https://doi.org/10.1007/s11746-015-2604-9

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