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Detection, Identification, and Characterization of Eimeria spp. from Commercial Chicken Farms in Different Parts of Iran by Morphometrical and Molecular Techniques

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Abstract

Purpose

Coccidiosis of domestic chicken is an important disease caused by any of seven species of Eimeria which, by develo** within the epithelial cells of the intestine, cause lesions therein. We carried out a study on poultry farms located in various regions of Iran to determine the incidence and spread of Eimeria species by employing a single PCR test.

Methods

A total of 64 fully confirmed clinically intestinal tracts were collected from different parts of Iran. From these 64 intestinal tracts, 82 samples were prepared from the different sites involved in the digestive tract. In morphological assessment, 23 samples could not be isolated and its information was not evaluated.

Results

Using morphological methods, the following seven species of Eimeria were identified: E. acervulina (15/59; 25.42%), E. tenella (30/59; 50.84%), E. maxima (12/59; 20.33%), E. praecox (1/59; 1.69%), E. necatrix (2/59; 3.38%), E. mitis (5/59; 8.47%), and E. mivati (2/59; 3.38%). Mixed infections were found in eight (13.55%) samples. In molecular assessment, 31 samples could not be isolated and its information was not evaluated. Totally, the following five species were identified using molecular methods: E. acervulina (35/51; 68.62%), E. tenella (33/51; 64.70%), E. maxima (6/51; 11.76%), E. brunetti (5/51; 9.80%), and E. necatrix (2/51; 3.92%). Mixed infections were found in 23 (45.09%) samples.

Conclusions

The present study is an update on the situation of poultry coccidiosis in Iran and provides the first data on the molecular detection, identification, and characterization of Eimeria spp. in the poultry population of this country and confirmed the presence of different species of this parasite in this area. According to the results, E. acervulina and E. tenella, as the main disease-causing species, should be considered in control programs such as treatment and vaccination strategies.

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Acknowledgements

The authors would like to thank all people who helped in performing the research. We gratefully acknowledge Alireza Sazmand from the Department of Pathobiology, Bu-Ali Sina University, Iran, for his help in preparing samples from Yazd and Gorgan provinces.

Funding

This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.

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Authors and Affiliations

  1. Protozoology Laboratory, Parasitology Department, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran

    Vahid Nasiri & Farnoosh Jameie

  2. Quality Control Department, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran

    Hassan Morovati Khamsi

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  1. Vahid Nasiri
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Contributions

VN and FJ were involved in the conception of the research idea and methodology design and supervision, performed data analysis and interpretation, and were involved in the methodology and data analysis and VN, FJ, and HM prepared and critically revised the manuscript for publication and revision. All authors read and approved the final manuscript.

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Correspondence to Vahid Nasiri.

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Nasiri, V., Jameie, F. & Morovati Khamsi, H. Detection, Identification, and Characterization of Eimeria spp. from Commercial Chicken Farms in Different Parts of Iran by Morphometrical and Molecular Techniques. Acta Parasit. 69, 854–864 (2024). https://doi.org/10.1007/s11686-024-00818-x

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