Abstract
Total phenols, flavonoids, procyanidins, and total antioxidant capacity, measured with ferric reducing antioxidant power, radical scavenging capacity, and oxygen radical absorption capacity assays were first evaluated in the extracts of the shells, skins and kernels of 10 varieties of Pinus koraiensis. Results indicate that these varieties had strong radical scavenging capacities, ferric reducing antioxidant power and oxygen radical absorption capacities. Phenolic, flavonoid and procyanidin values ranged from 138.6 (#3 kernel) to 518.6 (#10 shell) mg GAE/g, from 23.3 (#2 kernel) to 70.8 (#5 skin) mg RE/g, from 2.5 (#2 kernel) to 142.1 (#7 skin) mg CE/g, respectively. Radical scavenging capacity and ferric reducing antioxidant power values were positively correlated to the polyphenol contents which play a major role in antioxidant properties. The varieties may be divided into two groups by cluster analysis and the variables being measured. These results will be useful for breeding varieties and guiding their production.
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References
Adom KK, Sorrells ME, Liu RH (2005) Phytochemicals and antioxidant activity of milled fractions of different wheat varieties. J Agric Food Chem 53:2297–2306
Bai J, Yuan QS, Li YL (2014) Study on bacteriostatic activity and stability of pinebark oligomeric proanthocyanidins. Chin J Biochem Pharm 34:55–60
Blomhoff R, Carlsen MH, Andersen LF, Jacobs JDR (2006) Health benefits of nuts: potential role of antioxidants. Br J Nutr 96:52–60
Cai J (2012) The extraction, purification, antioxidant activity and identification of flavonoids from pericarp of hickory nut (dissertation). Anhui Agricultural University, Anhui
Cai J, Cheng JW, Wang R, Ding WP, Wu YN (2016) Comparison of various methods for measuring antioxidant activities of polypeptide in vitro. Food Sci 37:52–57
Chang C, Ruan QJ, Bao YH, Guo Y, Shen HL (2017) Fatty acid composition and antioxidant activity of pinenut oils from different mountains. J Northeast Forestry Univ 45:84–87
Deng XR, Wang ZY, Liu R, Liu JH, OuYang L (2014) Antioxidant activity of ethanol extract from Picea koraiensis cone. J Bei**g Forestry Univ 36:94–101
Destaillats F, Cruz-Hernandez C, Giuffrida F, Dionisi F (2010) Identification of the botanical origin of pine nuts found in food products by gas–liquid chromatography analysis of fatty acid profile. J Agric Food Chem 58:2082–2087
Dong ZY, Ren H, Zhou YJ (2011) Optimization onultrasonic-assisted extraction technology of flavonoids from pine nut shell with response surface analysis. Food Mach 27:100–102
Du J, Li HT, Zheng GY (2012) Study on ultrasonic extraction technology of procyanidin from Pine bark. Res Pract Chin Med 26:62–65
Fan ZL, Chen KL, Gao X, Bao YH (2017a) Extraction optimization and antioxidant activity of polyphenols from the needles of five pine species. Mod Food Sci Technol 33:212–220
Fan ZL, Zhang YD, Zhang H, Wang ZY, Bao YH (2017b) Antioxidant and antibacterial activity of essential oil from Pinus koraiensis needles. J Bei**g Forestry Univ 39:98–103
French CJ, Elder M, Leggett F, Ibrahim RK, Ghn T (1991) Flavonoids inhibit infectivity of tobacco mosaic virus. Can J Plant Pathol 13:1–6
Gentile C, Tesoriere L, Butera D, Fazzari M, Monastero M, Allegra M, Livrea MA (2007) Antioxidant activity of sicilian pistachio (Pistacia vera L. var. bronte) nut extract and its bioactive components. J Agric Food Chem 55:643–648
Hoon LY, Choo C, Watawana MI, Jayawardena N, Waisundara VY (2015) Evaluation of the total antioxidant capacity and antioxidant compounds of different solvent extracts of Chilgoza pine nuts (Pinus gerardiana). J Funct Food 18:1014–1021
Hou J (2015) Research on the polyphenols extraction and purification of pine nut coat and its antioxidant characteristics (dissertation). Harbin University of Commerce, Harbin
Huang DJ, Ou B, Hampsch-Woodill M, Flanagan JA, Prior RL (2002) High-throughput assay of oxygen radical absorbance capacity (ORAC) using a multichannel liquid handing system coupled with a microplate fluorescence reader in 96-well format. J Agric Food Chem 50:4437–4444
Huang SM, Zhang J, Fan YQ, Mou LH (2012) Optimization of extraction process for proanthocyanidins from fruits of Caryota mitis lour. Food Sci 33:104–108
Huang SR, Yang XN, Zhang L, Zheng MX, **e JS, Fu CL (2014) Optimized extraction and antioxidant activity of proanthocyanidins from longan pericarp. Food Sci 35:68–75
Hyeusoo K, Byongsoon L, Kyeong WY (2013) Comparison of chemical composition and antimicrobial activity of essential oils from three Pinus species. Ind Crop Prod 44:323–329
İbrahim T, Esra KA, Hakkı T, Ipek S, Mehmet K (2018) Research on the antioxidant, wound healing, and anti-inflammatory activities and the phytochemical composition of maritime pine (Pinus pinaster Ait). J Ethnopharmacol 211:235–246
Li B, Bao YH, Gao F, Wang ZY (2014) Purification of polyphenols from Korean pine cone lamella by SephadexLH-20 and their antioxidative activities in vitro. Sci Technol Food Ind 35:57–61
Ma CH, Wang Q, Liu M (2016) Evaluation of antioxidant activity of pine polyphenols from three Pinaceae species in vitro. Southwest China. J Agric Sci 29:1063–1067
Miraliakbari H, Shahidi F (2008) Antioxidant activity of minor components of tree nut oils. Food Chem 111:421–427
Noumi E, Snouss M, Hajlaoui H, Trabelsi N, Ksouri R, Valentin E, Bakhrouf A (2011) Chemical composition, antionxidant and antifungal potential of melaleuca alternifolia (tea tree) and eucalyptus globulus essential oils against oral Candida s-pecies. J Med Plants Res 5:4147–4156
Osman U, Fatma SS, Mine K, Ilkay EO, Murat K, Kemal HCB (2012) Investigation on chemical composition, anticholinesterase and antioxidant activities of extracts and essential oils of Turkish Pinus species and pycnogenol. Ind Crops Prod 38:115–123
Pereira JA, Oliveira I, Sousa A, Ferreira ICFR, Bento A, Estevinho L (2008) Bioactive properties and chemical composition of six walnut (Juglans regia L.) varieties. Food Chem Toxicol 46:2103–2111
Piao ZJ (2013) Pinus koraiensis. Forest Humankind 6:94–97
Sang S, Lapsley K, Jeong W, Lachance PA, Ho CT, Rosen RT (2002) Antioxidative phenols compounds isolated from almond skins (Prunus amygdalus Batsch). J Agric Food Chem 50:2459–2463
Su XY, Wang ZY, Liu J (2009) In vitro and in vivo antioxidant activity of Pinus koraiensis seed extract containing phenols compounds. Food Chem 117:681–686
Tsantili E, Konstantinidis K, Christopoulos MV, Roussos PA (2011) Total phenolics and flaconoids and total antionxidant capacity in pistachio (Pistachis vera L.) nuts in relation to varieties and storage conditions. Sci Hortic 129:694–701
Wang ZY, **g QJ, Cheng CL (2006) Study on the oxidation resistance of constituents extracted from the seed-shell of Korean Pine by supercritical CO2. Biomass Chem Eng 40:40–43
Wang JX, Huan YF, Zha XY, Sun M, Xu YZ, Peng LX, Liu Y (2013) Determination of total flavonoids in buckwheat and buckwheat goods. Sci Technol Food Ind 34:58–60
Wang WJ, Lu JL, Du HJ, Wei CH, Wang HM, Fu YJ, He XY (2017) Ranking thirteen tree species based on their impact on soil physiochemical properties, soil fertility, and carbon sequestration in Northeastern China. Forest Ecol Manag 404:214–229
Wen P, Wang ZY, Pei ZS (2016) Study on optimized ultrasonic-assisted extraction process of total flavonoids from pine nut shell of Pinus koraiensis by response surface methodology. China Food Addit 6:73–79
Wu XH, Yang CP (2015) The effects of antioxidant capacity pine nut protein in mice. J Chin Cereals Oils Assoc 30:28–31
**e K, Miles EA, Calder PC (2016) A review of the potential health benefits of pine nut oil and its characteristic fatty acid pinolenic acid. J Funct Foods 23:464–473
Xu DY, Yan H (2001) A study of the impacts of climate change on the geographic distribution of Pinus koraiensis in China. Environ Int 27:201–205
Yang J, Liu RH, Halim L (2009) Antioxidant and antiproliferative activities of common edible nut seeds. Food Sci Technol 42:1–8
Yi JJ, Wang ZY, Bai HN, Yu XJ, **g J, Zuo LL (2015) Optimization of purification, identification and evaluation of the in vitro antitumor activity of polyphenols from Pinus Koraiensis Pinecones. Molecules 20:10450–10467
Yi JJ, Qu H, Wu YZ, Wang ZY, Wang L (2017) Study on antitumor, antioxidant and immunoregulatory activities of the purified polyphenols from pinecone of Pinus koraiensis on tumor-bearing S180 mice in vivo. Int J Biol Macromol 94:735–744
Zeng L, Fu LY, Luo LY, Ma MJ, Li S (2015) Principal component and cluster analyses of volatile components in tea flowers from different varieties at different stages of bloom. Food Sci 36:88–93
Zhang GS, Hou J, Zhang MD, Di MF (2014) Research of extraction and antioxidant activity in vitro of polyphenols from pine nut coat. Sci Technol Food Ind 35:252–256
Zheng HL, He F, Teng F, Wan P, Wang ZY, Ma SH (2014) Study on the optimization of extraction technology and the antioxidant effect of proanthocyanidins from Picea koraiensis Nakai’s cones. Sci Technol Food Ind 35:258–263
Zhou DY, Shen G (2003) Characters and distribution regulations of Korean pine-broad leaf mixed forest in the northeast of China. Territory Nat Resour Study 2:91–92
Zorica SM, Boris J, Snežana ČJ, Tatjana MK, Zorica ZSR, Vladimir JC, LjM Tatjana, Petar DM, Bojan KZ, Gordana SS (2018) Comparative study of the essential oils of four Pinus species: chemical composition, antimicrobial and insect larvicidal activity. Ind Crop Prod 111:55–62
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The authors thank Dr. Kai Yang for the supply of pine nuts from the Forestry Science Institute of Heilongjiang Province.
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Project funding: The work was supported by the National Key Research and Development Program of China during the “13th 5-Year Plan” (Grant No. 2016YFC0500307-07) and a Grant from the Application Technology Research and Development Program of Harbin (Grant No. 2017RALXJ001).
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Corresponding editor: Tao Xu.
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Zhang, S., Zhang, L., Wang, L. et al. Total phenols, flavonoids, and procyanidins levels and total antioxidant activity of different Korean pine (Pinus koraiensis) varieties. J. For. Res. 30, 1743–1754 (2019). https://doi.org/10.1007/s11676-018-0744-0
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DOI: https://doi.org/10.1007/s11676-018-0744-0