SpringerLink
Log in

Acute toxic effects of thiamethoxam on Chinese mitten crab Eriocheir sinensis

  • Research Article
  • Published:
Environmental Science and Pollution Research Aims and scope Submit manuscript

Abstract

The information about toxic effects of thiamethoxam on non-target aquatic organisms is still incomplete. The semi-static toxicity test method was used to investigate the acute toxic effects of thiamethoxam on Eriocheir sinensis. The results showed that the median lethal concentration (LC50) of thiamethoxam to E. sinensis at 96 h was 510 μg/L, and the safety concentration (SC) was 51 μg/L. After 96 h exposure to thiamethoxam, the survival rates of crabs at concentrations of 0, 151.11, 226.67, 340, and 510 μg/L were 100%, 76.19%, 64.29%, 61.91%, and 46.43%, respectively. A significant (P < 0.05) decrease of the number of hemocytes was observed in thiamethoxam groups. With the increase of thiamethoxam concentration, the phagocytic activity of hemocytes, the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), total antioxidant capacity (T-AOC), and the activities of protease, amylase, and lipase of crabs increased firstly and then decreased, and the above indexes reached the maximum in 151.11 μg/L thiamethoxam group. Collectively, a high concentration of thiamethoxam (510 μg/L) had a great effect on the gene expression of immune metabolism-related factors in hepatopancreas and gill of crabs. These findings indicated that thiamethoxam exposure had the ability to impair immune and metabolic systems and resulted in the reduction of survival rate of crabs.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price includes VAT (Germany)

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

Data availability

The datasets generated during the current study are available from the corresponding author on reasonable request.

References

  • Ashida M (1971) Purification and characterization of prophenoloxidase from hemolymph of the silkworm Bombyx mori. Arch Biochem Biophys 144:749–762

    Article  CAS  Google Scholar 

  • Basley K, Goulson D (2018) Neonicotinoids thiamethoxam and clothianidin adversely affect the colonisation of invertebrate populations in aquatic microcosms. Environ Sci Pollut R 25:9593–9599

    Article  CAS  Google Scholar 

  • Burella PM, Odett LM, Simoniello MF, Poletta GL (2018) Oxidative damage and antioxidant defense in Caiman latirostris (Broad-snouted caiman) exposed in ovo to pesticide formulations. Ecotoxicol Environ Saf 161:437–443

    Article  CAS  Google Scholar 

  • Cui QM, Cai FF, Zhan WH, Yuan CY (2019) Effect of Chlorella polysaccharide on hemocyte phagocytosis and gene expression of immune related factors in Penaeus vannamei. Feed Res 42:42–45

    Google Scholar 

  • Cui QM, Zhao ZY, Yuan CY (2020) Effects of astragalus polysacharin on hemocyte phagocytosis and gene expression of immune related factors in Eriocheir sinensis. Aquacult Int 28:1787–1796

    Article  CAS  Google Scholar 

  • Finnegan MC, Baxter LR, Maul JD, Hanson ML, Hoekstra PF (2017) Comprehensive characterization of the acute and chronic toxicity of the neonicotinoid insecticide thiamethoxam to a suite of aquatic primary producers, invertebrates, and fish. Environ Toxicol Chem 36:2838–2848

    Article  CAS  Google Scholar 

  • Gao T, Sun Y, Cui QM, Yuan CY (2021) Effects of compound feed attractant on growth performance, digestive enzyme activity and intestinal flora composition of Litopenaeus vannamei. Feed Res 44:43–46

    Google Scholar 

  • Gauthier M, Aras P, Paquin J, Boily M (2018) Chronic exposure to imidacloprid or thiamethoxam neonicotinoid causes oxidative damages and alters carotenoid-retinoid levels in caged honey bees (Apis mellifera). Sci Rep 8:1–11

    Google Scholar 

  • Ge QQ (2013) Effects on the Imd pathway genes expression and non-specific immunity of Fenneropenaeus chinensis and Exopalaemon carinicauda by Vibrio anguillarum. Ocean University of China, Qing Dao

    Google Scholar 

  • Geng XB, Shen MF, Wu GH, Gao YY, Ge XQ, Song HB (2009) Acute toxicity effects of Chinese mitten crabs on deltamethrin. J Aquac 13:48–50

    Google Scholar 

  • Golovanova AL, Aminov AI, Filippov AA (2013) Effects of organic pollutants on fish digestive enzymes: a review. Inland Water Biol 6:155–160

    Article  Google Scholar 

  • Hong YH, Yang XZ, Huang Y, Yan GW, Cheng YX (2018a) Assessment of the oxidative and genotoxic effects of the glyphosate-based herbicide roundup on the freshwater shrimp, Macrobrachium nipponensis. Chemosphere 210:896–906

    Article  CAS  Google Scholar 

  • Hong YH, Huang Y, Yan GW, Huang ZQ (2019a) Effects of deltamethrin on the antioxidant defense and heat shock protein expression in Chinese mitten crab, Eriocheir sinensis. Environ Toxicol Pharmacol 66:1–6

    Article  CAS  Google Scholar 

  • Hong YH, Yang XZ, Yan GW, Huang Y, Zuo F, Shen YX, Ding Y, Cheng YX (2017) Effects of glyphosate on immune responses and haemocyte DNA damage of Chinese mitten crab, Eriocheir sinensis. Fish Shellfish Immunol 71:19–27

    Article  CAS  Google Scholar 

  • Hong YH, Huang Y, Yan GW, Pan C, Zhang JL (2019b) Antioxidative status, immunological responses, and heat shock protein expression in hepatopancreas of Chinese mitten crab, Eriocheir sinensis under the exposure of glyphosate. Fish Shellfish Immunol 86:840–845

    Article  CAS  Google Scholar 

  • Hong YH, Yang XZ, Huang Y, Yan GW, Cheng YX (2018b) Oxidative stress and genotoxic effect of deltamethrin exposure on the Chinese mitten crab, Eriocheir sinensis. Comp Biochem Physiol C 212:25–33

    CAS  Google Scholar 

  • Hong YH, Yin HM, Huang Y, Huang Q, Yang XZ (2020) Immune response to abamectin-induced oxidative stress in Chinese mitten crab, Eriocheir sinensis. Ecotoxicol Environ Saf 188:1–7

    Article  Google Scholar 

  • Huang Y, Hong YH, Huang ZQ, Zhang JL, Huang Q (2019) Avermectin induces the oxidative stress, genotoxicity, and immunological responses in the Chinese Mitten Crab, Eriocheir sinensis. PloSone 14:e0225171

    Article  CAS  Google Scholar 

  • Ji S, Cheng HS, Ding W, Lu JQ, Zhou Y (2020) Research progress on the pollution status of neonicotinoids pesticides in environmental systems. Shan Dong Chem Ind 49:64–66

    Google Scholar 

  • Jiang RL, **ao BC, Zhang F, Feng DX, Yu N, Du ZY, Chen LQ (2015) Acute toxic effects of phenanthrene on Chinese mitten crab Eriocheir sinensis. Chin J Appl Environ Biol 21:108–114

    CAS  Google Scholar 

  • Li YH, Wang MJ, Ma BH, Li ZH, Wang HH (2021) Acute toxicity of three conventional aquatic chemicals on juvenile Anabas testudineus. Fish Sci Technol Inform 48:94–98

    Google Scholar 

  • Liu XL, Zhou ZL, Chen LQ (2003) Effect of cadmium on antioxidant enzyme activities of the juvenile Eriocheir sinensis. Marine Sci 27:9–63

    Google Scholar 

  • Mao ZF, Lin QC, Wang BY, Zhang CZ, Chen ZH, Jian SQ, Zhao DX (2018) Effects of Cu2+ stress on toxicity and expression of immune related genes in Chinese mittencrab, Eriocheir sinensis. Acta Agr Uni Jiangxi 40:358–364

    Google Scholar 

  • Rane M, Mahajan AY (2013) Effect of thiamethoxam on oxygen consumption of the freshwater bivalve, Lamellidens Marginalis (Lamarck). The Bioscan 8:469–472

    Google Scholar 

  • Wang Z, Peng S, Meng LX (2020) Acute toxicity of thiamethoxam and activities of antioxidant enzymes and acetylcholinesterase in juvenile crucian carp Carassius auratus. Fish Sci 39:922–927

    Google Scholar 

  • Xu WJ, Pan LQ, Yue F, Li J (2011) Effects of ammonia-N stress on digestive enzyme activities of swimming crab Portunus trituberculatus. Period Ocean Uni China 41:35–40

    CAS  Google Scholar 

  • Yang XZ, Song YM, Zhang C, Pang YY, Song XZ, Wu MY, Cheng YX (2019) Effects of the glyphosate-based herbicide roundup on the survival, immune response, digestive activities and gut microbiota of the Chinese mitten crab, Eriocheir sinensis. Aquat Toxicol 214:1–8

    Article  Google Scholar 

  • Yang ZB (2005) Studies on the toxic mechanism of water-borne Cu2+ on Eriocheir Sinensis. East China Noraml University, Shang Hai

    Google Scholar 

  • Yang ZY, Zhang YL, Hu K, Yang XL, Liu LS, Zhang FX, Cai HG (2017) Effects of deltamethrin exposure on oxidative stress indexes and histological structure of hepatopancreas in Eriocheir sinensis. Acta Agr Zhe Jiang 29:1261–1270

    Google Scholar 

  • Yao CL, Wang ZY, **ang JH (2006) Crustacean haemocytes and their function in immune responses. Zool Res 27:549–557

    Google Scholar 

  • Zhang Y (2010) Function and application of immune related factors from shrimp Litopenaes vannamei and crab Eriocheir sinensis Institute of Oceanology. Chinese Academy of Sciences, Qing Dao

    Google Scholar 

  • Zhang MF, Fan JY, Zhang HW, Zhang XZ, Ma XG (2009) Research progress of neonicotinoid pesticides. World Pesticides 31:22–25

    CAS  Google Scholar 

  • Zhang XJ, Lu HD, Tian QQ, Jia XX, Ren FF (2018) Effects of deltamethrin on histopathology of Chinese mitten crab Eriocheir sinensis. Asian J Ecotoxicol 13:342–351

    Google Scholar 

  • Zhou X, Qiu Y, Li XL, Xu ZQ, Zhang JP (2019) Effects of thiamethoxam and chlorantraniliprole on the growth and development of non-target pests Tetranychus turkestani. **njiang Agr Sci 56:23–31

    Google Scholar 

  • Zhuang P, Wang RF, Feng GP, Zhang LZ, Huang XR, Zhao F, Wang Y (2013) The response of digestive enzyme activity in the mature Chinese mitten crab, Eriocheir sinensis (Decapoda: Brachyura), to gradual increase of salinity. Sci Mar 77:323–329

    Article  Google Scholar 

Download references

Funding

This work was financially supported by Tian** Science and Technology Project (20YFZCSN00140) and Innovation Team for Tian** Modern Agricultural Industry Technical System (ITTMRS2021009).

Author information

Authors and Affiliations

  1. College of Marine and Environmental Sciences, Tian** University of Science and Technology, Tian**, 300457, China

    Yue Sun, Chunying Yuan & Qingman Cui

Authors
  1. Yue Sun
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Chunying Yuan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Qingman Cui
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

Yue Sun performed the experiment; Chunying Yuan contributed to the conception of the study and wrote the manuscript; Qingman Cui performed the data analyses and manuscript preparation.

Corresponding author

Correspondence to Chunying Yuan.

Ethics declarations

Ethics approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed by the authors.

Conflict of interest

The authors declare no competing interests.

Additional information

Responsible Editor: Bruno Nunes

Publisher's note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Sun, Y., Yuan, C. & Cui, Q. Acute toxic effects of thiamethoxam on Chinese mitten crab Eriocheir sinensis. Environ Sci Pollut Res 29, 63512–63519 (2022). https://doi.org/10.1007/s11356-022-20294-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11356-022-20294-y

Keywords

Search

Navigation