Abstract
Water Treatment Plants (WTP) and Sewage Treatment Plants (STP) generate residues known as sludge (WS and SS, respectively). SS and WS present some positive characteristics for reuse in agriculture. The aim of the present study was to evaluate, using the Allium cepa test, the effectiveness of the bioremediation process in the detoxification of SS and WS sludges. In this study, the phytotoxic, cytotoxic, genotoxic, and mutagenic potentials of pure sludge samples (WS and SS) were evaluated, as well as the association of these two sludges with soil (S), before and after the bioremediation process. In the T0 period (before undergoing bioremediation), the SS, SS + S, and SS + WS samples totally inhibited the germination of A. cepa, proving the high phytotoxic potential of these samples. For the T1 period (after 6 months of bioremediation), phytotoxicity was observed for the SS, SS + S, SS + WS, and SS + WS + S samples, but there was not a complete inhibition of germination and radicles growth, allowing the evaluation of the other parameters (cytogenotoxic and mutagenic potential). No cytotoxicity was observed for any sample, both in T0 and T1. As for the genotoxicity parameter, a significant result was observed for the pure WS sample in T0 and for all samples in T1, when compared to NC. The genotoxic alteration most found in meristematic cells exposed to treatments was of binucleated cells. Mutagenic potential was also observed for samples of WS and WS + S in T0. From this study, we can conclude that, after six months of bioremediation, despite the SS phytotoxicity being reduced, all samples were genotoxic to the A. cepa organism test.
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This work was supported by the São Paulo Research Foundation (FAPESP) [2017/13031-2].
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This work was supported by São Paulo Research Foundation (FAPESP) Process number: 2017/13031–2.
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Tamara Santos: Conceptualization, Investigation, Formal analysis, Writing—Original Draft, Writing—Review & Editing, Visualization and Funding acquisition. Lais Roberta Deroldo Sommaggio: Conceptualization, Formal analysis, Visualization and Writing—Review & Editing. Maria Aparecida Marin-Morales: Conceptualization, Methodology, Resources, Writing—Review & Editing, Supervision, Funding acquisition. All authors read and approved the final manuscript.
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Santos, T., Sommaggio, L.R.D. & Marin-Morales, M.A. Phyto-genotoxicity assessment of different associations between sludges from Water and Sewage Treatment Plants, before and after the bioremediation process. Environ Sci Pollut Res 29, 40029–40040 (2022). https://doi.org/10.1007/s11356-022-18820-z
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DOI: https://doi.org/10.1007/s11356-022-18820-z