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Ligninolytic enzyme involved in removal of high molecular weight polycyclic aromatic hydrocarbons by Fusarium strain ZH-H2

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Abstract

The capacity of Fusarium sp. strain ZH-H2 to secret lignin peroxidase (LiP), laccase (Lac), and manganese peroxidase (MnP) and degrade high molecular weight polycyclic aromatic hydrocarbons (HMW-PAHs) was studied. When the fungus was grown in control mineral salt medium for 4 days, LiP and Lac activities were detected at 8871 U L−1 and 5123 U L−1, respectively. In the presence of HMW-PAHs as the sole carbon source, only LiP activity was detectable, and LiP activity had significantly reduced HMW-PAHs at day 7, with a maximum decrease of 85.9%. A strong correlation between LiP activity and HMW-PAHs removal efficiency could be fit into various models, with the highest correlation coefficients obtained for quadratic functions (P < 0.01). When a specific enzyme inhibitor was added, the ability of Fusarium to remove HMW-PAHs was reduced from 85.9 to 66.7%, depending on the inhibitor’s concentration. Meanwhile, the determined activity of LiP was reduced from 11.4 to 48.6%. We conclude that in the presence of HMW-PAHs as the only carbon source to support growth, Fusarium ZH-H2 mainly produces LiP but not Lac or MnP for HMW-PAHs degradation. To our knowledge, it was the first time to propose a metabolic lignin peroxidase characterization of HMW-PAHs degradation by Fusarium sp. strains.

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Change history

  • 28 August 2020

    The article Ligninolytic enzyme involved in removal of high molecular weight polycyclic aromatic hydrocarbons by Fusarium strain ZH-H2, written by **aoxue Zhang, **aomin Wang, Cheng Li, Lixiu Zhang, Guohui Ning, Wei Shi, Xuena Zhang and Zhixin Yang, was originally published electronically on the publisher’s internet portal (currently SpringerLink) on 28 July 2020 with open access.

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Funding

This study was supported by Key R&D Program in Hebei Province (19223811D), the National key R&D Program of China (2018YFD0800402), the Educational Commission of Hebei Province of China (Z2013058), and the National High-Tech R&D Program of China (863 Program) (2012AA101403).

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Author notes

  1. **aoxue Zhang and **aomin Wang contributed equally to this study and share first authorship.

Authors and Affiliations

  1. College of Resource and Environmental Sciences, Agricultural University of Hebei, 2596 Lekai South Street, Baoding, 0710001, People’s Republic of China

    **aoxue Zhang, **aomin Wang, Cheng Li, Lixiu Zhang, Guohui Ning, Wei Shi & Zhixin Yang

  2. Tian** **qing Economic-Technological Development Area, Tian**, 0710001, People’s Republic of China

    Xuena Zhang

  3. Key Laboratory for Farmland Eco-Environment, Hebei Province, Agricultural University of Hebei, Baoding, People’s Republic of China

    Zhixin Yang

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  1. **aoxue Zhang
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  2. **aomin Wang
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  3. Cheng Li
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  4. Lixiu Zhang
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  5. Guohui Ning
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  6. Wei Shi
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  7. Xuena Zhang
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Correspondence to Zhixin Yang.

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Zhang, X., Wang, X., Li, C. et al. Ligninolytic enzyme involved in removal of high molecular weight polycyclic aromatic hydrocarbons by Fusarium strain ZH-H2. Environ Sci Pollut Res 27, 42969–42978 (2020). https://doi.org/10.1007/s11356-020-10192-6

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