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Environmentally Relevant Concentrations of TiO2 Nanoparticles Affected Cell Viability and Photosynthetic Yield in the Chlorophyceae Scenedesmus bijugus

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Abstract

The impact of nanoparticles (NPs) in phytoplankton is understudied, particularly with respect to the organism’s physiology and environmentally relevant concentrations. In the present research, we investigated the effects of titanium dioxide nanoparticles (nano-TiO2) in the physiology of Scenedesmus bijugus, a freshwater cosmopolitan phytoplankter, exposed to concentrations ranging from 3.30 × 10−9 mol L−1 (log −8.48) to 3.70 × 10−7 mol L−1 (log −6.43), which includes environmentally relevant values. The nano-TiO2 concentrations in the medium and in the cells were determined in experiments that lasted 96 h. Controlled environmental conditions were used throughout and a variety of endpoints were monitored. These included cell density, cell viability, chlorophyll a concentration, growth rates, maximum quantum yield of photosystem II (ΦM), intracelular proteins and carbohydrates, and proteins:carbohydrates ratios. The results showed that cell viability was the most sensitive parameter for the detection of the nano-TiO2 effects, being followed by ΦM. At the concentration of 3.90 × 10−8 mol L−1 (log −7.40), there was an increase of nano-TiO2 injured cells, and at 3.70 × 10−7 mol L−1 (log −6.43) 24%, ΦM decrease in comparison with the controls was obtained. Different from several literature results, we showed that nano-TiO2 particles at environmentally relevant concentrations affected microalgae physiology, and this was dependent on the endpoint used to evaluate the effect.

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Acknowledgments

We thank Prof. Dr. Edson Roberto Leite and collaborators for NP characterization, and Prof. Dr. Joaquim A. Nobrega and Dr. Clarisse D. B. Amaral for Ti determinations. ATL thanks Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq—Brazil) (302175/2015-6) for financial support and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior – Demanda Social (Capes-DM) for a scholarship.

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  1. Department of Botany, Programa de Pós-Graduação em Ecologia e Recursos Naturais Rodovia Washington Luis, Federal University of São Carlos, km 235, SP 310 – CEP, São Carlos, 13565-905, Brazil

    Daniela Mariano Barreto & Ana Teresa Lombardi

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  1. Daniela Mariano Barreto
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  2. Ana Teresa Lombardi
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Correspondence to Daniela Mariano Barreto.

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Barreto, D.M., Lombardi, A.T. Environmentally Relevant Concentrations of TiO2 Nanoparticles Affected Cell Viability and Photosynthetic Yield in the Chlorophyceae Scenedesmus bijugus . Water Air Soil Pollut 227, 450 (2016). https://doi.org/10.1007/s11270-016-3139-x

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