Abstract
The contamination of hazardous metal(loid) is one of the serious environmental and human health risks. This study isolated a total of 40 cadmium (Cd)- and arsenic (As)-resistant bacterial isolates from coastal sediments by pour plate technique using tryptic soy agar supplemented with Cd or As (50 mg l−1) for use as metal(loid) bioremediation agents. Out of 40, 4 isolates, RCd3, RCd6, RAs7, and RAs10, showed a relatively higher growth rate in Cd- or As-supplemented culture media which were selected for further study. The selected isolates showed a high minimum inhibitory concentration (60–400 mg l−1 for Cd and 400–2200 mg l−1 for As), which demonstrated their remarkable Cd and As resistance capabilities. The metal(loid) removal efficiencies (0.032–0.268 μg Cd h−1 mg−1 and 0.0003–0.0172 μg As h−1 mg−1 [wet weight cell]) of selected isolates indicated their greater magnitude in absorbing Cd compared to As from water. Phylogenetic analysis of the 16S rDNA sequences revealed that isolates RCd3, RCd6, RAs7, and RAs10 were closely related to Acinetobacter brisouii, Pseudomonas abietaniphila, Exiguobacterium aestuarii, and Planococcus rifietoensis, respectively. Because of high Cd and As resistance and removal efficiency, the selected isolates can survive in a high metal(loid)-contaminated environment and could be a potential tool for bioremediation of high metal(loid)-contaminated effluents to protect the aquatic environment.
Similar content being viewed by others
References
Aksu, Z. (2005). Application of biosorption for the removal of organic pollutants: a review. Process Biochemistry, 40(3–4), 997–1026.
Altschul, S. F., Gish, W., Miller, W., Myers, E. W., & Lipman, D. J. (1990). Basic local alignment search tool. Journal of Molecular Biology, 215(3), 403–410.
Amundsen, P. A., Staldvik, F. J., Lukin, A. A., Kashulin, N. A., Popova, O. A., & Reshetnikov, Y. S. (1997). Heavy metal contamination in freshwater fish from the border region between Norway and Russia. The Science of the Total Environment, 201(3), 211–224.
Belimov, A. A., Hontzeas, N., & Safronova, V. I. (2005). Cadmium-tolerant plant growth-promoting bacteria associated with the roots of Indian mustard (Brassica juncea L. Czern.). Soil Biology and Biochemistry, 37(2), 241–250.
Bhakta, J. N., & Munekage, Y. (2010). Spatial distribution and contamination status of arsenic, cadmium and lead in some coastal shrimp (Macrobrachium rosenbergii) farming ponds of Viet Nam. Pacific Journal of Science and Technology, 11(1), 606–615.
Bhakta, J. N., Munekage, Y., Ohnishi, K., & Jana, B. B. (2012a). Isolation and identification of cadmium and lead resistant lactic acid bacteria for applying as metal removing probiotic. International journal of Environmental Science and Technology, 9(3), 433–440.
Bhakta, J. N., Ohnishi, K., Munekage, Y., Iwasaki, K., & Wei, M. (2012b). Characterization of lactic acid bacteria-based probiotics as heavy metals sorbents. Journal of Applied Microbiology, 112(6), 1193–1206.
Bhakta, J. N., Majumdar, B. P., & Munekage, Y. (2014). Development of activated carbon from cotton fibre waste as potential mercury adsorbent: kinetic and equilibrium studies. Chemical Engineering Journal, 2014, 7.
Brown, M. J., & Lester, J. N. (1982). Role of bacterial extracellular polymers in metal uptake in pure bacterial culture and activated sludge—1. Effects of metal concentration. Water Research, 16(11), 1539–1548.
Campbell, P. G. C. (2006). Cadmium—a priority pollutant. Environmental Chemistry, 3, 387–388.
Choudhury, H., & Mudipalli, A. (2008). Potential considerations & concerns in the risk characterization for the interaction profiles of metals. The Indian Journal of Medical Research, 128, 462–483.
Edwards, P. (2005a). Demise of peri-urban wastewater-fed aquaculture? Urban Agriculture Magazine, 14, 27–29.
Edwards, P. (2005b). Development status of, and prospects for, wastewater-fed aquaculture in urban environments. In B. Costa-Pierce, A. Desbonnet, P. Edwards, & D. Baker (Eds.), Urban aquaculture (pp. 45–59). Wallingford: CABI.
Elsilk, S. E., El-Shanshoury, A. E. R., & Ateya, P. S. (2014). Accumulation of some heavy metals by metal resistant avirulent Bacillus anthracis PS2010 isolated from Egypt. Africa Journal of Microscopy Research, 8(12), 1266–1276.
Gadd, G. M. (1990). Heavy metal accumulation by bacteria and other microorganisms. Experientia, 46(8), 834–840.
Giller, K., Witter, E., & McGrath, S. P. (1998). Toxicity of heavy metals to microorganisms and microbial processes in agricultural soils: a review. Soil Biology and Biochemistry, 30(10–11), 1389–1414.
Gupta, U. C., & Gupta, S. C. (1998). Trace element toxicity relationships to crop production and livestock and human health: implications for management. Communications in Soil Science and Plant Analysis, 29(11–14), 1491–1522.
Hemond, H. F., & Solo-Gabriele, H. M. (2004). Children’s exposure to arsenic from CCA-treated wooden decks and playground structures. Risk Analy, 24(1), 51–64.
Hrudey, S. E., Chen, W., & Rousseaux, C. G. (1995). Bioavailability in environmental risk assessment. Boca Raton: Lewis Publ.
Idris, R., Trifonova, R., & Puschenreiter, M. (2004). Bacterial communities associated with flowering plants of the Ni hyperaccumulator Thlaspi goesingense. Applied and Environmental Microbiology, 70(5), 2667–2677.
International Occupational Safety and Health Information Centre. (1999). Metals in basics of chemical safety (7th ed.). Geneva: International Labour Organization.
Kim, O. S., Cho, Y. J., Lee, K., Yoon, S. H., Kim, M., Na, H., et al. (2012). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. International Journal of Systematic and Evolutionary Microbiology, 62(Pt 3), 716–721.
Kratochvil, D., & Volesky, D. (1998). Advances in the biosorption of heavy metals. Tybtech, 16(7), 291–300.
Larkin, M. A., Blackshields, G., Brown, N. P., Chenna, R., McGettigan, P. A., McWilliam, H., et al. (2007). Clustal W and Clustal X version 2.0. Bioinformatics, 23(21), 2947–2948.
Lee, S.-H., Chang, K.-S., Su, M.-S., Huang, Y.-S., & Jang, H.-D. (2007). Effects of some Chinese medicinal plant extracts on five different fungi. Food Control, 18, 1547–1554.
Long, A., & Wang, W. (2005). Assimilation and bioconcentration of Ag and Cd by the marine black bream after waterborne and dietary metal exposure. Environmental Toxicology and Chemistry, 24(3), 709–716.
Mathivanan, K., & Rajaram, R. (2014). Tolerance and biosorption of cadmium (II) ions by highly cadmium resistant bacteria isolated from industrially polluted estuarine environment. Indian Journal of Geo-Marine Sciences, 43(4), 580–588.
Min-sheng, H., **g, P., & Le-**, Z. (2001). Removal of heavy metals from aqueous solutions using bacteria. Journal of Shanghai University, 5(3), 253–259.
Murphy, B. L., Toole, A. P., & Bergstrom, P. D. (1989). Health risk assessment for arsenic contaminated soil. Environ. Geochem. Heal., 11(3–4), 163–169.
Navarro, C. A., von Bernath, D., & Jerez, C. A. (2013). Heavy metal resistance strategies of acidophilic bacteria and their acquisition: importance for biomining and bioremediation. Biological Research, 46(4), 363–371.
Pazirandeh, M., Wells, B. M., & Ryan, R. L. (1998). Development of bacterium-based heavy metal biosorbents: enhanced uptake of cadmium and mercury by Escherichia coli expressing a metal binding motif. Applied and Environmental Microbiology, 64(10), 4068–4072.
Phuong, N. T. D., & Tuan, P. A. (2005). Current status of periurban aquatic production in Hanoi. Urban Agriculture Magazine, 14, 10–12.
Qing, H., Min-Na, D., Hong-Yan, Q., **ang-Ming, X., Guo-Qiang, Z., & Min, Y. (2007). Detection, isolation, and identification of cadmium-resistant bacteria based on PCR-DGGE. Journal of Environmental Sciences, 19(9), 1114–1119.
Roane, T. M., & Kellogg, S. T. (1996). Characterization of bacterial communities in heavy metal contaminated soils. Canadian Journal of Microbiology, 42(6), 593–603.
Roberts, J. R. (1999). Metal toxicity in children. In Training manual on pediatric environmental health: putting it into practice. Emeryville: Children’s Environmental Health Network. http://www.cehn.org/cehn/trainingmanual/pdf/manual-full.pdf. Accessed 23 Aug 2013.
Ruangsomboon, S., & Wongrat, L. (2006). Bioaccumulation of cadmium in an experimental aquatic food chain involving phytoplankton (Chlorella vulgaris), zooplankton (Moina macrocopa), and the predatory catfish Clarias macrocephalus and C. gariepinus. Qualitative Toxicity, 78(1), 15–20.
Ruiz-Barba, J. L., Maldonado, A., & Jiménez-Díaz, R. (2005). Small-scale total DNA extraction from bacteria and yeast for PCR applications. Analytical Biochemistry, 347(2), 333–335.
Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M., & Kumar, S. (2011). MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution, 28(10), 2731–2739.
Trakranrungsie, N., Chatchawanchonteera, A., & Khunkitti, W. (2007). Ethnoveterinary study for antidermatophytic activity of Piper betle, Alpinia galanga and Allium ascalonicum extracts in vitro. Research in Veterinary Science, 84(1), 80–84.
USEPA. (1992). Common chemicals found at superfund sites. Minerals Engineering, 14, 317–340. U.S. Gov Print Office, Washington, DC.
Yamina, B., Tahar, B., & Marie Laure, F. (2012). Isolation and screening of heavy metal resistant bacteria from wastewater: a study of heavy metal co-resistance and antibiotics resistance. Water Science and Technology, 66(10), 2041–2048.
Acknowledgments
The authors are grateful to the government of Japan for sponsoring the grant-in-aid for scientific research fund to carry out the present study. Dr. Bhakta is also especially grateful to Japan Society for Promotion of Science (JSPS) for providing the fellowship under the “FY2009 JSPS postdoctoral fellowship for foreign researcher.”
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Bhakta, J.N., Munekage, Y., Ohnishi, K. et al. Isolation and Characterization of Cadmium- and Arsenic-Absorbing Bacteria for Bioremediation. Water Air Soil Pollut 225, 2151 (2014). https://doi.org/10.1007/s11270-014-2151-2
Received:
Accepted:
Published:
DOI: https://doi.org/10.1007/s11270-014-2151-2