Abstract
Trace minerals participate in reproductive processes and are crucial for oocyte maturation. The objective of the present study was to investigate the effect of combined supplementation with copper (Cu), manganese (Mn), selenium (Se) and zinc (Zn) during bovine in vitro maturation (IVM) on subsequent embryo development and quality. The IVM medium was supplemented as follows: a) Control (no mineral supplementation); b) MScz (6 ng/mL Mn + 100 ng/mL Se + 200 ng/mL Cu + 400 ng/mL Zn); c) MScZ (6 ng/mL Mn + 100 ng/mL Se + 200 ng/mL Cu + 1200 ng/mL Zn); d) MSCz (6 ng/mL Mn + 100 ng/mL Se + 600 ng/mL Cu + 400 ng/mL Zn). Supplementation with MScz and MSCz produced more blastocysts compared with the control. Total blastocyst cell number was higher when minerals were added at any combination. Day-8 blastocysts derived from oocytes treated with minerals had lower intracellular reactive oxygen species concentration and lipid content than the control. In conclusion, combined supplementation with Cu, Mn, Se and Zn during bovine oocyte IVM increased in vitro production performance, improving embryo developmental ability and quality.
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The datasets in this study are available from the corresponding author on reasonable request.
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Acknowledgements
We are grateful to the staff of Frigorífico Gorina S.A. for providing bovine ovaries, to the staff of “Fundación La Margarita Zemborain – Grondona” for supplies, and to Centro de Inseminación Artificial EOLIA S.A. for providing bovine frozen semen. We also thank A. Di Maggio for manuscript correction
Funding
This research was supported by Agencia Nacional de Promoción Científica y Tecnológica de la República Argentina (MINCyT) [Grants PICT 2018–1451 and PICT 2018–4716].
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J.P. Anchordoquy: Methodology, Investigation, Validation, Writing—original draft, Resources. M. Balbi: Investigation. N.A. Farnetano: Investigation. M.C. Fabra: Investigation. A.C. Carranza-Martin: Investigation, Validation N. Nikoloff: Investigation, Validation. G. Giovambattista: Investigation. C.C. Furnus: Writing—original draft, Funding acquisition. J.M. Anchordoquy: Methodology, Formal analysis, Investigation, Funding acquisition, Writing—review & editing.
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Anchordoquy, J.P., Balbi, M., Farnetano, N.A. et al. Trace mineral mixture supplemented to in vitro maturation medium improves subsequent embryo development and embryo quality in cattle. Vet Res Commun 46, 1111–1119 (2022). https://doi.org/10.1007/s11259-022-09982-9
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DOI: https://doi.org/10.1007/s11259-022-09982-9