Abstract
The ability of non-native plant species to tolerate water scarcity and high temperatures, especially during the early stages of development, may influence their invasive potential. Ligustrum lucidum is a tree native to warm temperate areas of Asia. To understand the potential expansion of this non-native invasive species into more arid regions, we conducted an experiment in incubation chambers to evaluate seed germination under different water potentials and the response of its seedlings under different water availability and temperature scenarios. Seeds were collected from central Argentina and placed to germinate under four different water potentials (−0.2; −0.4; −0.7 and −1.2 MPa) created with polyethylene glycol 6000. Additionally, L. lucidum seedlings were subjected to contrasting levels of water availability combined with different periods of exposure to high temperatures. Germination measurements (germination percentage, velocity, and synchronicity) decreased at lower water potentials. No germination occurred at −0.7 and −1.2 MPa. The seedlings showed a slight increase in mortality and a significant reduction in most of the growth variables under low water availability, while exposure to high temperatures had significant effects only on chlorophyll estimate. The germination response of L. lucidum to water deficits could pose a significant constraint on its establishment in arid ecosystems. Nonetheless, this constraint may be alleviated by the species’ prolific propagule production, coupled with asynchronous seed germination, which may help it exploit more humid micro-sites or sporadic water events. Furthermore, the drought tolerance of its seedlings strongly warns against the use of this species as an ornamental plant in arid environments.
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References
Aragón R, Groom M (2003) Invasion by Ligustrum lucidum (Oleaceae) in NW Argentina: early stage characteristics in different habitat types. Rev Biol Trop 51:59–70
Arias G, Zeballos SR, Ferreras AE (2023) Competition effect exerted by two nonnative invasive plant species on a native under contrasting conditions of resource availability. Biol Inv 25:2261–2276. https://doi.org/10.1007/s10530-023-03039-x
Baskin CC, Baskin JM (2014) Seeds: ecology, biogeography, and evolution of dormancy and germination, 2nd edn. Elsevier/Academic Press, San Diego
Bauk K, Flores J, Ferrero C, Pérez-Sánchez R, Las Peñas ML, Gurvich DE (2017) Germination characteristics of Gymnocalycium monvillei (Cactaceae) along its entire altitudinal range. Botany 95:419–428. https://doi.org/10.1139/cjb-2016-0154
Bellis LM, Astudillo A, Gavier-Pizarro G, Dardanelli S, Landi M, Hoyos L (2021) Glossy privet (Ligustrum lucidum) invasion decreases chaco serrano forest bird diversity but favors its seed dispersers. Biol Inv 23:723–739. https://doi.org/10.1007/s10530-020-02399-y
Bewley JD, Bradford KJ, Hilhorst HW, Nonogaki H (2013) Germination. Seeds: physiology of development, germination and dormancy, 3rd edn. Springer, New York
Brixner Dreyer JBB, Higuchi P, Silva AC (2019) Ligustrum lucidum WT Aiton (broad-leaf privet) demonstrates climatic niche shifts during global-scale invasion. Sci Rep 9:3813. https://doi.org/10.1038/s41598-019-40531-8
Budiman J, Bahrawi J, Hidayatulloh A, Almazroui M, Elhag M (2021) Volumetric quantification of flash flood using microwave data on a watershed scale in arid environments Saudi Arabia. Sustainability 13:4115. https://doi.org/10.3390/su13084115
Burrows FJ, Kohen J (1983) Germination of Ligustrum lucidum WT Ait. and L. sinense Lour. at different temperatures. Australian Weed 2:130–132
Cony MA, Trione SO (1996) Germination with respect to temperature of two Argentinian prosopis species. J Arid Environ 33:225–236. https://doi.org/10.1006/jare.1996.0058
De Fina AL (1992) Aptitud agroclimática de la República Argentina. Academia Nacional de Argonomía y Veterinaria, Buenos Aires
Diez JM, D’Antonio CM, Dukes JS, Grosholz ED, Olden JD, Sorte CJ, Blumenthal DM, Bradley BA, Early R, Ibáñez I, Jones SJ, Lawler JJ, Miller LP (2012) Will extreme climatic events facilitate biological invasions? Front Ecol Environ 10:249–257. https://doi.org/10.1890/110137
Djumaeva D, Lamers JPA, Martius C, Vlek PLG (2012) Chlorophyll meters for monitoring foliar nitrogen in three tree species from arid Central Asia. J Arid Environ 85:41–45. https://doi.org/10.1016/j.jaridenv.2012.03.008
Donohue K, Rubio de Casas R, Burghardt L, Kovach K, Willis CG (2010) Germination, postgermination adaptation, and species ecological ranges. Annu Rev Ecol Evol Syst 41:293–319. https://doi.org/10.1146/annurev-ecolsys-102209-144715
Eyster HN, Wolkovich EM (2021) Comparisons in the native and introduced ranges reveal little evidence of climatic adaptation in germination traits. Clim Change Ecol 2:100023. https://doi.org/10.1016/j.ecochg.2021.100023
Fan B, Zhou Y, Ma Q, Yu Q, Zhao C, Sun K (2018) The bet-hedging strategies for seedling emergence of Calligonum mongolicum to adapt to the extreme desert environments in northwestern China. Front Plant Sci 9:1167
Fenner M, Thompson K (2005) The ecology of seeds. Cambridge University Press, Cambridge, UK; New York.
Fernandez RD, Ceballos SJ, Aragón R, Malizia A, Montti L, Whitworth-Hulse JI, Castro-Diez P, Grau HR (2020) A global review of Ligustrum lucidum (OLEACEAE) invasion. Bot Rev 86:93–118. https://doi.org/10.1007/s12229-020-09228-w
Ferreras AE, Torres C, Galetto L (2008) Fruit removal of an invasive exotic species (Ligustrum lucidum) in a fragmented landscape. J Arid Env 72:1573–1580. https://doi.org/10.1016/j.jaridenv.2008.03.015
Ferreras AE, Whitworth-Hulse JI, Tecco PA, Marcora PI, Funes G (2019) Environmental constraints tonative woody species recruitment in invaded mountain woodlands of central Argentina. Forest Ecol Manag 440:189–201. https://doi.org/10.1016/j.foreco.2019.03.022
Furey C, Tecco PA, Perez-Harguindeguy N, Giorgis MA, Grossi M (2014) The importance of native and exotic plant identity and dominance on decomposition patterns in mountain woodlands of central Argentina. Acta Oecol 54:13–20. https://doi.org/10.1016/j.actao.2012.12.005
Giorgis MA, Cingolani AM, Gurvich DE, Tecco PA, Chiapella J, Chiarini F, Cabido M (2017) Changes in floristic composition and physiognomy are decoupled along elevation gradients in central Argentina. Appl Veg Sci 20:558–571. https://doi.org/10.1111/avsc.12324
Godoy O, de Lemos-Filho JP, Valladares F (2011) Invasive species can handle higher leaf temperature under water stress than mediterranean natives. Environ Exp Bot 7:207–214. https://doi.org/10.1016/j.envexpbot.2010.12.001
Gurvich DE, Pérez-Sánchez R, Bauk K, Jurado E, Ferrero MC, Funes G, Flores J (2017) Combined effect of water potential and temperature on seed germination and seedling development of cacti from a mesic Argentine ecosystem. Flora 227:18–24. https://doi.org/10.1016/j.flora.2016.12.003
Hirsch H, Hensen I, Wesche K, Renison D, Wypior C, Hartmann M, von Wehrden H (2016) Non-native populations of an invasive tree outperform their native conspecifics. AoB Plants 8:plw071. https://doi.org/10.1093/aobpla/plw071
Hou QQ, Chen BM, Peng SL, Chen LY (2014) Effects of extreme temperature on seedling establishment of nonnative invasive plants. Biol Inv 16:2049–2061. https://doi.org/10.1007/s10530-014-0647-8
Hoyos LE, Gavier-Pizarro GI, Kuemmerle T, Bucher EH, Radeloff VC, Tecco PA (2010) Invasion of glossy privet (Ligustrum lucidum) and native forest loss in the Sierras Chicas of Córdoba, Argentina. Biol Inv 12:3261–3275. https://doi.org/10.1007/s10530-010-9720-0
Hu XW, Fan Y, Baskin CC, Baskin JM, Wang YR (2015) Comparison of the effects of temperature and water potential on seed germination of Fabaceae species from desert and subalpine grassland. Am J Bot 102:649–660. https://doi.org/10.3732/ajb.1400507
Khurana EKTA, Singh JS (2001) Ecology of seed and seedling growth for conservation and restoration of tropical dry forest: a review. Environ Conserv 28:39–52. https://doi.org/10.1017/S0376892901000042
Kitajima K, Fenner M (2000) Ecology of seedling regeneration. Seeds: the ecology of regeneration in plant communities. CABI publishing, Wallingford, pp 331–359
Lipiec J, Doussan C, Nosalewicz A, Kondracka K (2013) Effect of drought and heat stresses on plant growth and yield: a review. Int Agrophys 27:463–477. https://doi.org/10.2478/intag-2013-0017
Lozano-Isla F, Benites-Alfaro OE, Pompelli MF (2019) GerminaR: an R package for germination analysis with the interactive web application “GerminaQuant for R.” Ecol Res 34:339–346. https://doi.org/10.1111/1440-1703.1275
Maestre FT, Benito BM, Berdugo M, Concostrina-Zubiri L, Delgado-Baquerizo M, Eldridge DJ, Guirado E, Gross N, Kéfi S, Le Bagousse-**uet Y, Ochoa-Hueso R, Soliveres S (2021) Biogeography of global drylands. New Phytol 231:540–558. https://doi.org/10.1111/nph.17395
Malizia A, Osinaga-Acosta O, Powell PA, Aragón R (2017) Invasion of Ligustrum lucidum (Oleaceae) in subtropical secondary forests of NW Argentina: declining growth rates of abundant native tree species. J Veg Sci 28:1240–1249. https://doi.org/10.1111/jvs.12572
Marcora PI, Zeballos SR, Ferreras AE, Arias G, Hensen I, Tecco PA (2023) Drought and herbivory as modulators of intraspecific differentiation in seedlings of a mountain tree. Plant Ecol 224:895–903. https://doi.org/10.1007/s11258-023-01345-x
Mazzolari AC, Millan EN, Bringa EM, Vázquez DP (2020) Modeling habitat suitability and spread dynamics of two invasive rose species in protected areas of Mendoza Argentina. Ecol Complex 44:100868. https://doi.org/10.1016/j.ecocom.2020.100868
Michel BE, Kaufmann MR (1973) The osmotic potential of polyethylene glycol 6000. Plant Physiol 51:914–916
Montti L, Velazco SJE, Travis JM, Grau HR (2021) Predicting current and future global distribution of invasive Ligustrum lucidum WT Aiton: assessing emerging risks to biodiversity hotspots. Diver Distrib 27:1568–1583. https://doi.org/10.1111/ddi.13303
Natale E, Zalba SM, Oggero A, Reinoso H (2010) Establishment of Tamarix ramosissima under different conditions of salinity and water availability: Implications for its management as an invasive species. J Arid Environ 74:1399–1407. https://doi.org/10.1016/j.jaridenv.2010.05.023
Parsons RF (2012) Incidence and ecology of very fast germination. Seed Sci Res 22:161–167. https://doi.org/10.1017/S0960258512000037
Plaza Behrh MC, Pérez CA, Goya JF, Azcona M, Arturi MF (2016) Plantación de Celtis ehrenbergiana como técnica de recuperación de bosques invadidos por Ligustrum lucidum en los talares del NE de Buenos Aires. Ecol Austral 26:171–177
Poorter H, Niklas KJ, Reich PB, Oleksyn J, Poot P, Mommer L (2012) Biomass allocation to leaves, stems and roots: meta-analyses of interspecific variation and environmental control. New Phytol 193:30–50
R Development Core Team (2023) R: a language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. https://www.R-project.org/
Ranal MA, García Santana D (2006) How and why to measure the germination process? Braz J Bot 29:1–11. https://doi.org/10.1590/S0100-84042006000100002
Robertson PA, Mill A, Novoa A, Jeschke JM, Essl F, Gallardo B, Geist J, Jaric I, Lambin X, Musseau C, Pergl J, Pysek P, Rabitsch W, von Schmalensee M, Shirley M, Strayer DL, Stefansson RA, Smith K, Booy O (2020) A proposed unified framework to describe the management of biological invasions. Biol Inv 22:2633–2645. https://doi.org/10.1007/s10530-020-02298-2
Shaygan M, Baumgartl T, Arnold S (2017) Germination of Atriplex halimus seeds under salinity and water stress. Ecol Eng 102:636–640. https://doi.org/10.1016/j.ecoleng.2017.02.050
Slatyer RO (1957) The influence of progressive increases in total soil moisture stress on transpiration, growth, and internal water relationships of plants. Aust J Biol Sci 10:320–336
Tecco PA, Gurvich DE, Díaz S, Pérez‐Harguindeguy N, Cabido M (2006) Positive interaction between invasive plants: the influence of Pyracantha angustifolia on the recruitment of native and exotic woody species. Austral Ecol 31:293–300. https://doi.org/10.1111/j.1442-9993.2006.01557.x
Tecco PA, Pais-Bosch AI, Funes G, Marcora PI, Zeballos SR, Cabido M, Urcelay C (2016) Mountain invasions on the way: are there climatic constraints for the expansion of alien woody species along an elevation gradient in Argentina? J Plant Ecol 9:380–392. https://doi.org/10.1093/jpe/rtv064
Theoharides KA, Dukes JS (2007) Plant invasion across space and time: factors affecting nonindigenous species success during four stages of invasion. New Phytol 176:256–273. https://doi.org/10.1111/j.1469-8137.2007.02207.x
Torresín JA, Zamboni LP, Sione WF, Rodríguez E, Aceñolaza PG (2013) Modelado de la distribución espacial de árboles exóticos invasores (AEI) en el Parque Nacional Pre-Delta (Entre Ríos, Argentina). Multequina 22:3–13
Toscano S, Ferrante A, Tribulato A, Romano D (2018) Leaf physiological and anatomical responses of Lantana and Ligustrum species under different water availability. Plant Physiol Bioch 127:380–392. https://doi.org/10.1016/j.plaphy.2018.04.008
Venier P, Cabido M, Funes G (2017) Germination characteristics of five coexisting neotropical species of Acacia in seasonally dry Chaco forests in Argentina. Plant Spec Biol 32:134–146. https://doi.org/10.1111/1442-1984.12134
Venier P, Ferreras AE, Lauenstein DL, Funes G (2023) Nurse plants and seed provenance in the restoration of dry Chaco forests of central Argentina. Forest Ecol Manag 529:120638. https://doi.org/10.1016/j.foreco.2022.120638
Wahid A, Gelani S, Ashraf M, Foolad MR (2007) Heat tolerance in plants: an overview. Environ Exp Bot 61:199–223. https://doi.org/10.1016/j.envexpbot.2007.05.011
Whitworth-Hulse JI, Magliano PN, Zeballos SR, Gurvich DE, Spalazzi F, Kowaljow E (2020) Advantages of rainfall partitioning by the global invader Ligustrum lucidum over the dominant native Lithraea molleoides in a dry forest. Agr Forest Meteorol 290:108013. https://doi.org/10.1016/j.agrformet.2020.108013
Wilson SB, Knox GW, Nolan KL, Aldrich J (2014) Landscape performance and fruiting of 12 privet selections grown in northern and southern Florida. Hort Technol 24:148–155. https://doi.org/10.21273/HORTTECH.24.1.148
Acknowledgements
We thank Gustavo Bertone for technical assistance in setting up the experiment, Jorgelina Brasca for English edition and the suggestions of the editor and two anonymous reviewers, which really improved the manuscript. A.E. Ferreras, P.I. Marcora, P.A. Tecco, M.P. Venier, G. Funes and S.R. Zeballos are researchers at CONICET.
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This work was supported by Proyecto de Investigación plurianual-2023-1794-APN-DIR#CONICET.
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AEF conceived the idea, performed the experiment, performed data analysis and wrote the original draft. PIM and SRZ conceived the idea, performed the experiment, and reviewed and edited the original manuscript. PAT performed the experiment, and reviewed and edited the original manuscript. PV and GF reviewed and edited the original manuscript.
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Ferreras, A.E., Marcora, P.I., Tecco, P.A. et al. How far can it go? Tolerance of seeds and seedlings of an invasive tree to water deficit and high temperatures. Plant Ecol (2024). https://doi.org/10.1007/s11258-024-01443-4
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DOI: https://doi.org/10.1007/s11258-024-01443-4