Abstract
Two experiments were conducted to evaluate the effect of intramuscular administration of minerals during a TAI program on the reproductive responses of lactating Angus cows. All cows (n=353) were subjected to a 9-day TAI program based on CIDR insertion plus injections of estradiol, cloprostenol, and eCG, and then TAI 48 h later. In experiment 1, two groups were randomly created, one control with a placebo injection (CON, n=109), and the second received 10 mL of Fosfosan® (MIN, n=172) on day 0 of the synchronization. Conception rate (66.9 vs. 55%) and estrus percentage (55.8 vs. 44%) were higher (P≤0.05) in MIN than in CON cows. Given these results, a second experiment was conducted randomly assigning the cows to two treatments (n=36 each): a single injection of 10 mL of Fosfosan® (MIN-O) on day 0 or two injections of 10 mL of Fosfosan® (MIN-T) on synchronization days 0 and 7. Four cows of each treatment were randomly selected to be scanned with transrectal ultrasound before and during the synchronization protocol to assess ovarian structures and cyclicity, and at day 39 post-TAI for pregnancy diagnosis. Also, blood samples were obtained for the determination of serum minerals and progesterone (P4) concentrations. The number of mineral injections did not affect conception rate (P≥0.1229) conception rate, serum mineral and P4 concentrations, number, and size of emerging follicles, or follicle size according to 1 to 4 classifications. The MIN-T promoted (P<0.05) earlier follicular wave emergence than MIN-O. However, MIN-O cows had a dominant follicle of 15.12 mm, which is more significant (P<0.05) than that in MIN-T cows (13.5 mm). In conclusion, providing a single mineral injection of Fosfosan® at the start of a TAI program is an excellent reproductive strategy in lactating Angus cows to improve the dominant follicle growth, estrus response, and conception rate.
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Data availability
The data used to support the findings of this study are available from the corresponding author upon reasonable request.
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Acknowledgements
We thank the Virbac laboratories of México for providing the hormonal and mineral injections assessed in the study.
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The research group’s own funds and Virbac laboratories of México
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They conceived the experiment: Avendaño-Reyes Leonel and Hernández-Rivera Juan Augusto. They performed field trials: Mario González-López, Vielka Castañeda-Bustos, and Ángeles López-Baca. They performed statistical analysis and interpretation of results: Ulises Macías-Cruz, Omar Francisco Prado-Rebolledo, and García-Casillas Arturo César. Technical support and information about the hormone products and mineral injections used: Gonzalo López-Rincón. They wrote the manuscript: Avendaño-Reyes Leonel, Miguel Mellado, and Hernández-Rivera Juan Augusto. All authors read and approved the manuscript.
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Statement of animal rights. This study was conducted following approved local techniques of animal care (NOM-051-ZOO-1995: Humanitarian treatment of animals during mobilization).
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Avendaño-Reyes, L., González-López, M., Macías-Cruz, U. et al. Ovarian activity and reproductive responses of lactating Angus cows due to a mineral supplementation throughout a timed AI protocol. Trop Anim Health Prod 55, 308 (2023). https://doi.org/10.1007/s11250-023-03723-z
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DOI: https://doi.org/10.1007/s11250-023-03723-z