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Serum lipid, enzyme, and hematological responses of broilers fed Acacia angustissima leaf meal-based diets

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Abstract

The objective of the study was to determine serum lipid, enzyme, and hematological responses of broilers to Acacia angustissima leaf meal. Acacia angustissima leaves were harvested at mid maturity, ground into a leaf meal, and included in broiler diets at 0, 5, and 10%. One hundred and fifty-day-old chicks of 41.7 ± 1.560 g were randomly allocated to 15 groups of the three diets. Feed and water were provided ad libitum throughout the trial. At days 14, 28, and 42, 4 ml of blood were collected from the jugular vein in plain vacutainer tubes for alanine aminotransferase (ALT), alkaline phosphatase (ALP), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), and total cholesterol (TC) analyses. At the end of the experiment, 4 ml of blood were collected from two randomly selected birds of each replicate in sterilized bottles containing ethylene diamine tetra acetic acid (EDTA) for full blood count and packed cell volume assessment. Data was tested for normality and analyzed using the General Linear Model Procedure of SAS version 9.3 (SAS 2010). Comparison of means was done using Tukey’s test. A. angustissima had no effect on serum ALP, AST, LDH, TC, HDL-C, LDL-C, TG, full blood count, and packed cell volume. Increasing levels of A. angustissima leaf meal increased the ALT in the serum (p < 0.05) at 4 and 6 weeks. However, serum ALT indices for birds on the control and 5% diet were not different. It was concluded that 5% A. angustissima leaf meal can be included in broiler diets without a negative effect on serum lipid and enzyme profile, production and proportions of white blood cells and packed cell volume.

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Authors and Affiliations

  1. Faculty of Agriculture, Department of Animal Science, University of Zimbabwe, Box MP167, Mt Pleasant, Harare, Zimbabwe

    Sharai Ncube, Petronella Tapiwa Saidi, Venancio Edward Imbayarwo-Chikosi & Tinyiko Edward Halimani

  2. Faculty of Veterinary, Department of Clinical Veterinary Studies, University of Zimbabwe, Box MP167, Mt Pleasant, Harare, Zimbabwe

    Musavengana Tapera Tivapasi

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  1. Sharai Ncube
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  2. Petronella Tapiwa Saidi
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  3. Musavengana Tapera Tivapasi
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  4. Venancio Edward Imbayarwo-Chikosi
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Correspondence to Sharai Ncube.

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All procedures in this experiment followed guidelines by the Zimbabwe Scientific Animal Act, 1963, subsection 2 of section 4, License Number L624.

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Ncube, S., Saidi, P.T., Tivapasi, M.T. et al. Serum lipid, enzyme, and hematological responses of broilers fed Acacia angustissima leaf meal-based diets. Trop Anim Health Prod 50, 665–670 (2018). https://doi.org/10.1007/s11250-017-1484-4

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