Abstract
Light is one of the major abiotic factors which directly influence plant growth, development, and secondary metabolites accumulation in plants. The current study was designed to investigate the stimulatory effect of LEDs (light-emitting diodes), on biomass accumulation, pigment concentration, and bioactive metabolites production in Operculina turpethum callus culture. A 3.9-fold increase in the biomass accumulation was observed in callus culture grown under blue LED (FW: 13.1 ± 0.19 g/50 ml) whereas reduction in biomass was recorded for callus grown under dark conditions (FW: 2.1 ± 0.01 g/50 ml) when compared to the control (FW: 3.3 ± 0.04 g/50 ml). Furthermore, blue light was found to enhance the total phenolics (61.303 ± 0.32 mg GAE/g DW) and flavonoids (TFC: 26.48 ± 0.68 mg QE/g DW) synthesis in the callus culture among all the tested lights. Again, callus culture placed under blue light showed the highest in vitro antioxidant activities as compared to other lights. However, higher superoxide dismutase (SOD) and catalase (CAT) enzyme expression were observed for the callus culture grown under dark. Quantification of phytochemicals through HPLC revealed that optimum production of Gallic acid (3.3 ± 0.06 mg g−1 DW), Quercetin (0.92 ± 0.004 mg g−1 DW) Coumarin (5.3 ± 0.01 mg g−1 DW), and Salicylic acid (4.9 ± 0.03 mg g−1 DW) was observed under blue light treated callus cultures of O. turpethum. The results of our study demonstrated that the application of multispectral lights is a feasible alternative strategy for the enhanced biomass and secondary metabolite production in the callus culture of O. turpethum.
Key message
The application of abiotic elicitors such as light has been shown to be an effective alternative for the enhanced biomass production, stimulation of secondary metabolite content and in vitro antioxidant activity of callus culture of Operculina turpethum (L.)
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All data generated or analysed during this study are included in this published article.
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Acknowledgements
All authors thank Prof. (Dr.) Manojranjan Nayak, Founder & President, Siksha ‘O’ Anusandhan (Deemed to be University), Bhubaneswar, Odisha, India for providing the required infrastructure for the study. The authors are grateful to the Head, Centre for Biotechnology and Dean, School of Pharmaceutical Sciences, Siksha O Anusandhan (Deemed to be University) for the support.
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All authors contributed to the study conception and design. Media standardization and establishment of callus culture was performed by BB. The biochemical analysis was performed by BB, BJ. Data collection and statistical analysis were performed by BJ and AKG. Drafting and finalisation of the manuscript was carried out by BB, BJ and AKG. Supervision and critical revision were done by LA. All authors read and approved the final manuscript.
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Biswal, B., Jena, B., Giri, A.K. et al. Monochromatic light elicited biomass accumulation, antioxidant activity, and secondary metabolite production in callus culture of Operculina turpethum (L.). Plant Cell Tiss Organ Cult 149, 123–134 (2022). https://doi.org/10.1007/s11240-022-02274-9
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DOI: https://doi.org/10.1007/s11240-022-02274-9