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Induction of direct somatic embryogenesis and shoot organogenesis and histological study in tree peony (Paeonia sect. Moutan)

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Abstract

Tree peony (Paeonia sect. Moutan) is a world famous ornamental and economically important species, but it is recalcitrant to in vitro regeneration. Here, we present a protocol for induction of direct somatic embryogenesis (SE) and direct shoot organogenesis (SO) from zygotic embryos (ZEs) of Paeonia rockii and P. ostii. The results showed that explant genotypes, ZEs stages, sucrose and plant growth regulators (PGRs) have greatly influences on in vitro morphogenesis. The highest frequency of SE (48%) and mean number of somatic embryos (5) was obtained from mature ZE of P. rockii ‘**g Hong’ when cultured on modified Murashige and Skoog (mMS) medium supplemented with 2.22 µM BA and 0.23 M sucrose.The proliferation can be achieved by recurrent production of embryogenic callus and somatic embryos on mMS medium supplemented with 0.89 µM BA. The germinated rate of somatic embryos was 45% on WPM medium containing 2.22 µM BA and 1.44 µM GA3 after dormancy release at 4 °C under dark for 20 days. In addition, direct SO was obtained firstly in tree peony by using a variety of cytokinins. Morpho-histological analysis confirmed the initiation, development and reserve accumulation of somatic embryos and shoots. The study will be benificial to the propagation and breeding of tree peony.

Key message

Tree peony (Paeonia sect. Moutan) is a world famous ornamental and economically important species which is recalcitrant to in vitro regeneration. Direct somatic embryogenesis protocol of P. rockii ‘**g Hong’ was developed and morpho-histological analysis confirmed the initiation, development and reserve accumulation during in vitro morphogenesis. The study will provide valuable reference for propagation and breeding of tree peony.

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Abbreviations

ABA:

Abscisic acid

AC:

Activated carbon

AX:

Auxin

BA:

6-Benzyladenine

CH:

Casein hydrolysate

CK:

Cytokinin

CPPU:

N-(2-chloro-4-pyridyl)-N-phenylurea

IBA:

Indolebutyric acid

KT:

Kinetin

MS:

Murashige and Skoog

mT:

meta-Topolin

PUT:

Putrescine

SE:

Somatic embryogenesis

SO:

Shoot organogenesis

TDZ:

Thidiazuron

WPM:

Woody Plant Media

ZE:

Zygotic embryo

ZT:

Zeatin

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Funding

The study was supported by National key research and development projects (2019YFD1001502), Bei**g Science and Technology Planning Project (Z181100002518001), and the World-Class Discipline Construction and Characteristic Development Guidance Funds for Bei**g Forestry University (2019XKJS0324).

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Authors and Affiliations

  1. Bei**g Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, Bei**g Advanced Innovation Center for Tree Breeding by Molecular Design, National Engineering Research Center for Floriculture, School of Landscape Architecture, Peony International Institute, Bei**g Forestry University, Bei**g, 100083, China

    Yiming Du, Fangyun Cheng & Yuan Zhong

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  1. Yiming Du
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  2. Fangyun Cheng
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  3. Yuan Zhong
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Correspondence to Fangyun Cheng.

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The authors declare that they have no conflict of interest.

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Communicated by M. I. Beruto.

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Du, Y., Cheng, F. & Zhong, Y. Induction of direct somatic embryogenesis and shoot organogenesis and histological study in tree peony (Paeonia sect. Moutan). Plant Cell Tiss Organ Cult 141, 557–570 (2020). https://doi.org/10.1007/s11240-020-01815-4

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