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Differences in possible TCA cycle replenishing pathways in purple non-sulfur bacteria possessing glyoxylate pathway

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Abstract

Pathways replenishing tricarboxylic acid cycle were divided into four major groups based on metabolite serving as source for oxaloacetic acid or other tricarboxylic acid cycle component synthesis. Using this metabolic map, the analysis of genetic potential for functioning of tricarboxylic acid cycle replenishment pathways was carried out for seven strains of purple non-sulfur bacterium Rhodopseudomonas palustris. The results varied from strain to strain. Published microarray data for phototrophic acetate cultures of Rps. palustris CGA009 were analyzed to validate activity of the putative pathways. All the results were compared with the results for another purple non-sulfur bacterium, Rhodobacter capsulatus SB1003 and species-specific differences were clarified.

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Abbreviations

ICL:

Isocitrate lyase

ICDH:

Isocitrate dehydrogenase

PA:

Pyruvic acid

PGA:

Phosphoglyceric acid

PEP:

Phosphoenolpyruvate

TCA cycle:

Tricarboxylic acid cycle

OAA:

Oxaloacetic acid

BPG:

1,3-Bisphosphoglycerate

GAP:

Glyceraldehyde-3-phosphate

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Acknowledgements

The authors are grateful to Dr. Azat V. Abdullatypov for consulting, productive discussion, and careful revision of the manuscript. This work was conducted in the frame of project “Photosynthetic organisms as light energy transformers and valuable products producers” number АААА-А17-117030110141-2.

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Authors and Affiliations

  1. Institute of Basic Biological Problems, Russian Academy of Sciences, 2, Institutskaya Str, Pushchino, Moscow Region, Russia, 142290

    Ekaterina Petushkova & Anatoly Tsygankov

  2. Faculty of Biotechnology, Lomonosov Moscow State University, Leninskiye Gory 1, bld. 51, Moscow, Russia, 119991

    Sergei Iuzhakov

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  1. Ekaterina Petushkova
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  2. Sergei Iuzhakov
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  3. Anatoly Tsygankov
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Correspondence to Anatoly Tsygankov.

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Petushkova, E., Iuzhakov, S. & Tsygankov, A. Differences in possible TCA cycle replenishing pathways in purple non-sulfur bacteria possessing glyoxylate pathway. Photosynth Res 139, 523–537 (2019). https://doi.org/10.1007/s11120-018-0581-1

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