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Two complete mitochondrial genomes in Scolopendra and a comparative analysis of tRNA rearrangements in centipedes

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Abstract

Background

Centipedes are one of the oldest terrestrial arthropods belonging to the sub phylum Myriapoda. With the expansion of our understanding of the application of the two centipedes Scolopendra morsitans and Scolopendra hainanum, belonging to the order Scolopendromorpha, an exhaustive classification was required. Although consensus has been reached on the phylogeny of Chilopoda based on morphological traits, recent analyses based on molecular data exhibited differences in results.

Methods and results

The mitochondrial genome sequences of S. morsitans and S. hainanum were obtained by next-generation sequencing. S. morsitans contains 13 PCGs, two rRNAs, 11 tRNAs, and one CR. whereas S. hainanum contains 12 PCGs, of which ATP8 remains unpredicted, two rRNAs, 14 tRNAs, and one CR. An obvious tRNA rearrangement was found in the genus Scolopendra. S. morsitans exhibited a loss of trnW, trnC, trnI, trnK, trnD, trnA, trnN, trnQ, trnF, trnT, trnS, trnL, and trnV, and a repeat of trnR and trnL. S. hainanum exhibited a loss of trnQ, trnC, trnW, trnI, trnD, trnQ, trnP, and trnV. Phylogenetic analyses of centipedes based on 12 PCGs supported the sister relationship between the orders Geophilomorpha and Lithobiomorpha and a close relationship between Scolopendra dehaani and S. hainanum.

Conclusions

The new mitogenomes determined in this study provide new genomic resources for gene rearrangements and contribute to the understanding of the evolution of gene rearrangement in Chilopoda.

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Funding

This study was supported by the Higher Education Reform Project of Education Department of Jiangsu Province (2021JSJG249) and the Innovation and Entrepreneurship Training Program for College Students of China (202110298111Y).

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Authors and Affiliations

  1. The Co-Innovation Center for Sustainable Forestry in Southern China, College of Biology and the Environment, Nan**g Forestry University, No.159 Longpan Road, Nan**g, 210037, Jiangsu Province, People’s Republic of China

    Jiayu Ding, Wei Xu, Yining Chen, Han Wu, Haoming Jiang, Jiachen Wang, Yongbo Wu & Hongyi Liu

  2. Department of Agriculture, Zhejiang Open University, Hangzhou, 310030, China

    Hong Lan

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  1. Jiayu Ding
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  2. Hong Lan
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  3. Wei Xu
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Contributions

JD: funding acquisition, formal analysis, writing—original draft HL: methodology and formal analysis WX: formal analysis, writing–original draft. YC: validation. HW: validation. HJ: formal analysis. JW: formal analysis. YW: funding acquisition. HL: conceptualization, writing—review and editing, supervision.

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Correspondence to Hongyi Liu.

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The authors declare that they have no conflict of interest.

Ethical approval

This study was approved by the ethics committee of Nan**g Forestry University.

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No animals were killed in this study.

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Ding, J., Lan, H., Xu, W. et al. Two complete mitochondrial genomes in Scolopendra and a comparative analysis of tRNA rearrangements in centipedes. Mol Biol Rep 49, 6173–6180 (2022). https://doi.org/10.1007/s11033-022-07409-x

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