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RETRACTED ARTICLE: Testing alternatives: the use of adipose-derived mesenchymal stem cells to slow neurodegeneration in a rat model of Parkinson’s disease

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This article was retracted on 29 December 2023

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Abstract

Parkinson’s disease (PD) is a chronic neurodegenerative disease. Unfortunately, the effectiveness of anti-Parkinson treatments gradually diminishes owing to the progressive degeneration of the dopaminergic terminals. The research described here investigated the effect of adipose-derived mesenchymal stem cells (AD-MSC) versus that of an anti-Parkinson drug in a rat model of Parkinsonism. Forty adult rats were divided into four equal groups, each group receiving a different treatment: vehicle, rotenone, rotenone + AD-MSC, or rotenone + carbidopa/levodopa. Behavioral tests were carried out before and at the end of the treatment and specimens harvested from the midbrain were processed for light and electron microscopy. Genetic expression of glial fibrillary acidic protein (GFAP) and Nestin mRNA was assessed. Expression of the Lamin-B1 and Vimentin genes was measured, along with plasma levels of Angiopoietin-2 and dopamine. Treatment with rotenone induced pronounced motor deficits, as well as neuronal and glial alterations. The AD-MSC group showed improvements in motor function in the live animals and in the microscopic picture presented by their tissues. The fold change of both genes (GFAP and Nestin) decreased significantly in the AD-MSC and carbidopa/levodopa groups compared to the group with Parkinson’s disease. Plasma levels of Angiopoietin-2 and dopamine were significantly increased after treatment (P < 0.001) compared to levels in the rats with Parkinson’s disease. AD-MSC reduced neuronal degeneration more efficiently than did the anti-Parkinson drug in a rat model of Parkinsonism.

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Change history

Abbreviations

AD-MSC:

Adipose-derived mesenchymal stem cells

PD:

Parkinson’s disease

CP:

Cerebral peduncle

SN:

Substantia nigra

SNc:

Substantia nigra pars compacta

SNr:

Substantia nigra pars reticulate

TH:

Tyrosine hydroxylase

GFAP:

Glial fibrillary acidic protein

Vim:

Vimentin

Nes:

Nestin

FACS:

Fluorescence-activated cell sorting

BBB:

Blood–brain barrier

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Acknowledgments

The authors thank Professor Sanaa A. M.Elgayar for her support in electron microscopy processing and examination.

Funding

This research received no funding from any funding agencies.

Author information

Authors and Affiliations

  1. Department of Histology and Cell Biology, Faculty of Medicine, Assiut University, Assiut, Egypt

    Fatma Y. Meligy & Dalia A. Elgamal

  2. Department of Medical Physiology, Faculty of Medicine, Assiut University, Assiut, Egypt

    Eman S. H. Abd Allah

  3. Department of Medical Biochemistry, Faculty of Medicine, Assiut University, Assiut, Egypt

    Naglaa K. Idriss

  4. Department of Forensic Medicine and Clinical Toxicology, Faculty of Medicine, Assiut University, Assiut, Egypt

    Nagwa M. Ghandour

  5. Department of Plastic Surgery, Faculty of Medicine, Assiut University, Assiut, Egypt

    Ehab M. R. Bayoumy

  6. Department of Rehabilitation Sciences, College of Health and Rehabilitation Sciences, Princess Nourah Bint Abdulrahman University, Riyadh, KSA, Saudi Arabia

    Azza Sayed Abdelrehim Khalil

  7. Department of Anatomy and Embryology, Faculty of Medicine, Menoufia University, Menoufia, Egypt

    Mohamed M. El Fiky

  8. Department of Neurosurgery, Hackensack University Medical Center, Hackensack, NJ, USA

    Mostafa Elkhashab

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  1. Fatma Y. Meligy
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Correspondence to Dalia A. Elgamal.

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The study protocol was approved by the Ethical Committee of the Faculty of Medicine, Assiut University. Assiut, Egypt. Approval reference number; 17300077.

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The donors of the adipose tissue from which the MSCs were separated gave written informed consent prior to the study for their use and publication of the data.

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Meligy, F.Y., Elgamal, D.A., Abd Allah, E.S.H. et al. RETRACTED ARTICLE: Testing alternatives: the use of adipose-derived mesenchymal stem cells to slow neurodegeneration in a rat model of Parkinson’s disease. Mol Biol Rep 46, 5841–5858 (2019). https://doi.org/10.1007/s11033-019-05018-9

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